LOCUS       sequence-16920-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Francis NJ, Saurin AJ, Shao Z, Kingston RE
  TITLE     Reconstitution of a functional core polycomb repressive complex.
  JOURNAL   Mol Cell 2001 Sep;8(3):545-56.
  PUBMED    11583617
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2001-09"; volume: "8(3)"; pages: "545-56"
COMMENT     Sequence Label: pFastBac FPC
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..53)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(102..131)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(555..578)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgnnaannac atgcgctgag cttggactcg tggtggtggt gatgatgatg gtgggtcagc
       61 ggagattccg agcgccgtcc actgttgcgt tcggacttga tgtggtgatg atggtggtgg
      121 tggtgatggt gcttcttctc cttcttcgac tccttatcgt gggtagccga tgaggtggtg
      181 gcttgatgcg tgtccacatc gttttcggtg aaggtgtact catcctcctc ggattccggc
      241 tccggatcgg gttctttctc cttcttctta atgcctcgct tggagggagt tccggaggat
      301 ttgttcgttt gttcgtagat gtcgatgagg cggcgatcca ggatgtttac ctccggttcc
      361 caggtgttgt agcgctggtt ccagcccttc cacttgacac ggtactccac gacgcccttc
      421 ttaacgcgct tttggatgat tttctcagcc gcgtacacta gatcgactgg atcgtcggtc
      481 gcattgtcgc gccccaactt ccccttactc cccttgcctc gaccagtcat ttttataccg
      541 tcgctagagg atcctttgtc atcgtcgtcc ttgtagtcca tcccgatcca gatccactag
      601 tggcctatng ngcgcnaata gggtgcctcg tcnacgtagg cctttgaatc cgcgcgctnc
      661 ggaccggaat cgcgcccgat ggtgggacgg tatgaataat ccggaaaatt ataggttttt
      721 ataaaaaact gtaacnaaaa gaaannntat tnttggaang gt
//
