LOCUS       sequence-169203-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular
            linkers, and Tev protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN
  TITLE     Multiplexing Engineered Receptors for Multiparametric Evaluation of 
            Environmental Ligands.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2042-2055. doi: 
            10.1021/acssynbio.6b00279. Epub 2017 Aug 23.
  PUBMED    28771312
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00279"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2042-2055"
COMMENT     Sequence Label: Rap-MESA-20-Tev
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(158..160,161..481)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     misc_feature    543..595
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgccatca tgtgccgagc catctctctt aggcgcttgc tgctgctgct
      121 gctgcagctg tcacaactcc tagctgtcac tcaagggatg ggagtgcagg tggaaaccat
      181 ctccccagga gacgggcgca ccttccccaa gcgcggccag acctgcgtgg tgcactacac
      241 cgggatgctt gaagatggaa agaaatttga ttcctcccgg gacagaaaca agccctttaa
      301 gtttatgcta ggcaagcagg aggtgatccg aggctgggaa gaaggggttg cccagatgag
      361 tgtgggtcag agagccaaac tgactatatc tccagattat gcctatggtg ccactgggca
      421 cccaggcatc atcccaccac atgccactct cgtcttcgat gtggagcttc taaaactgga
      481 aagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg
      541 tcctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
      601 atctagaggc ggatctggag gctctggggg ttcaggcgga tcaggaggca gtggcgggtc
      661 aaccggtctg gtcgtggttg ctggagtcct gttttgttat ggcttgctag tgacagtggc
      721 tctttgtgtt gaaaacgaga gcttgtttaa ggggccgcgt gattacaacc cgatatcgag
      781 caccatttgt catttgacga atgaatctga tgggcacaca acatcgttgt atggtattgg
      841 atttggtccc ttc
//
