LOCUS       sequence-169205-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with rapamycin FRB ectodomain, 20 extracellular 
            linkers, M cleavage sequence, and Gal4 transactivator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN
  TITLE     Multiplexing Engineered Receptors for Multiparametric Evaluation of 
            Environmental Ligands.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2042-2055. doi: 
            10.1021/acssynbio.6b00279. Epub 2017 Aug 23.
  PUBMED    28771312
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00279"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2042-2055"
COMMENT     Sequence Label: Rap-MESA-20-M-Gal4
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             577..597
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gccatcatgt gccgagccat ctctcttagg
      121 cgcttgctgc tgctgctgct gcagctgtca caactcctag ctgtcactca agggatgctc
      181 gagatgtggc atgaaggcct ggaagaggca tctcgtttgt actttgggga aaggaacgtg
      241 aaaggcatgt ttgaggtgct ggagcccttg catgctatga tggaacgggg cccccagact
      301 ctgaaggaaa catcctttaa tcaggcctat ggtcgagatt taatggaggc ccaagagtgg
      361 tgcaggaagt acatgaaatc agggaatgtc aaggacctcc tccaagcctg ggacctctat
      421 tatcatgtgt tccgacgaat ctcaaagact agaggcggat ctggaggctc tgggggttca
      481 ggcggatcag gaggcagtgg cgggtcaacc ggtctggtcg tggttgctgg agtcctgttt
      541 tgttatggct tgctagtgac agtggctctt tgtgttgaaa acctgtattt tcagatgaag
      601 ctactgtctt ctatcgaaca agcatgcgat atttgccgac ttaaaaagct caagtgctcc
      661 aaagaaaaac cgaagtgcgc caagtgtctg aagaacaact gggagtgtcg ctactctccc
      721 aaaaccaaaa ggtctccgct gactagggca catctgacag aagtggaatc aaggctagaa
      781 agactggaac agctatttct actgattttt cctcgagaag accttgacat gattttgaaa
      841 atggattctt tacaggatat aa
//
