LOCUS       sequence-169206-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with rapamycin FRB ectodomain, 20 extracellular 
            linkers, M cleavage sequence, and Gal4 transactivator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN
  TITLE     Multiplexing Engineered Receptors for Multiparametric Evaluation of 
            Environmental Ligands.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2042-2055. doi: 
            10.1021/acssynbio.6b00279. Epub 2017 Aug 23.
  PUBMED    28771312
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00279"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2042-2055"
COMMENT     Sequence Label: Rap-MESA-20-M-Gal4
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(165..398)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 acaaaggaac ctttaataga aattggacag caagaaagcg agcttagtga tacttgtggg
       61 ccagggcatt agccacacca gccaccactt tctgataggc agcctgcact ggtggggtga
      121 attgctcgag gtttaaacag ctccaccgcg gtggcggccg cctacccacc gtactcgtca
      181 attccaaggg catcggtaaa catctgctca aactcgaagt cggccatatc cagagcgccg
      241 tagggggcgg agtcgtgggg ggtaaatccc ggacccgggg aatccccgtc ccccaacatg
      301 tccagatcga aatcgtctag cgcgtcggca tgcgccatcg ccacgtcctc gccgtctaag
      361 tggagctcgt cccccaggct gacatcggtc gggggggccg tcgacagtct gcgcgtgtgt
      421 cccgcgggga gaaaggacag gcgcggagcc gccagccccg cctcttcggg ggcgtcgtcg
      481 tccgggagat cgagcaggcc ctcgatggta gacccgtaat tgtttttcgt acgcgcgcgg
      541 ctgtaagcgc ttacagtcaa ctgtctttga cctttgttac tactctcttc cgatgatgat
      601 gtcgcactta ttctatgctg tctcaatgtt agaggcatat cagtctccac tgaagccaat
      661 ctatctgtga cggcatcttt attcacatta tcttgtacaa ataatcctgt taacaatgct
      721 tttatatcct gtaaagaatc cattttcaaa atcatgtcaa ggtcttctcg aggaaaaatc
      781 agtagaaata gctgttccag tctttctagc cttgattcca cttctgtcag atgtgcccta
      841 gtcagcg
//
