LOCUS       sequence-169207-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 
            1) in mammalian cells. Incorporates into viral membrane to label 
            pseudoviruses.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Miyauchi K, Marin M, Melikyan GB
  TITLE     Visualization of retrovirus uptake and delivery into acidic 
            endosomes.
  JOURNAL   Biochem J. 2011 Mar 15;434(3):559-69. doi: 10.1042/BJ20101588.
  PUBMED    21175427
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2011-03-15- 15"; volume: "434"; issue: "3"; 
            pages: "559-69"
COMMENT     Sequence Label: EcpH-TM
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..84
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             85..150
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             160..870
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(187..215)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     810..830
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagaat taattcacca tgtctgcact tctgatccta
       61 gctcttgttg gagctgcagt tgctgactac aaagaccatg acggtgatta taaagatcat
      121 gacatcgatt acaaggatga cgatgacaag cttctcgaga gtaaaggaga agaacttttc
      181 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      241 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      301 actactggaa aactacctgt tccttggcca acacttgtca ctactttaac ttatggtgtt
      361 caatgctttt caagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
      421 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      481 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
      541 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taacgatcac
      601 caggtgtaca tcatggcaga caaacaaaag aatggaatca aagctaactt caaaattaga
      661 cacaacattg aagatggagg cgttcaacta gcagaccatt atcaacaaaa tactccaatt
      721 ggcgatgggc ccgtcctttt accagacaac cattacctgt ttacaacttc tactctttcg
      781 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
      841 attacacatg gcatggatga actatacaag aattcacggt atgagattgt catcatcact
      901 gtggtagcag ccgcagtcat aatgggcact gcaggcctca gcacgtacct ctataaccgc
      961 cagcggtgag gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tc
//
