LOCUS       sequence-169212-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FLAG-HA-tagged human YTHDC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Patil DP, Chen CK, Pickering BF, Chow A, Jackson C, Guttman M, 
            Jaffrey SR
  TITLE     m6A RNA methylation promotes XIST-mediated transcriptional 
            repression.
  JOURNAL   Nature. 2016 Sep 7;537(7620):369-373. doi: 10.1038/nature19342.
  PUBMED    27602518
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-09-7- 7"; volume: "537"; issue: "7620"; pages:
            "369-373"
COMMENT     Sequence Label: pcDNA3-FLAG-HA-hYTHDC1
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             74..97
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             110..136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             110..136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttacc atggactaca aggacgacga tgacaagctc gatggaggat acccctacga
      121 cgtgcccgac tacgccagcg gatccactag taacggccgc cagtgtgctg gaattctgat
      181 ggcggctgac agtcgggagg agaaagatgg agaacttaat gttctggatg atattttaac
      241 tgaagtacca gaacaagatg atgaactgta taatccagag agtgaacaag ataaaaatga
      301 gaaaaaggga tcaaaaagaa aaagtgatcg aatggaatct actgatacca aacgacaaaa
      361 gccttctgtc cattcaagac aactggtttc taagccactg agctcatctg ttagcaataa
      421 caaaagaata gttagtacaa aaggaaagtc agccacagag tataaaaatg aggaatatca
      481 aagatctgaa agaaacaagc gtctagatgc tgatcggaaa attcgtctat caagtagtgc
      541 ctccagagaa ccttataaga atcaacctga aaaaacctgt gtccggaaaa gggatcctga
      601 aaggagggcc aaatctccta cgccagatgg ttctgagaga attgggcttg aagtggatag
      661 acgtgcaagc agatccagcc agtcttctaa ggaagaagtg aactctgaag aatatggctc
      721 tgaccatgag actggcagca gtggttcttc tgatgagcaa gggaacaaca ctgagaatga
      781 ggaggaagga gtggaagaag atgtggagga agatgaagaa gtagaagaag atgcagaaga
      841 agatgaagag gtggatgaag atggagagga ggaggaggaa gaggaga
//
