LOCUS       sequence-169213-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPRi/a V2 library parental plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, 
            Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS
  TITLE     Compact and highly active next-generation libraries for 
            CRISPR-mediated gene repression and activation.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760.
  PUBMED    27661255
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e19760. doi"; pages: " 
            10.7554/eLife.19760"
COMMENT     Sequence Label: pCRISPRia-v2
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(304..337)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     341..361
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        614..825
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
       61 cagcagagat ccagtttggt tagtaccggg cccgccctgc aggtctagag atccgacgcg
      121 ccatctctag gcccgcgccg gccccctcgc acggacttgt gggagaagct cggctactcc
      181 cctgccccgg ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat
      241 aaaagacaga taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt
      301 tctataactt cgtatagcat acattatacg aagttataaa cagcacaaaa ggaaactcac
      361 cctaactgta aagtaattgt gtgttttgag actataagta tcccttggag aaccaccttg
      421 ttggaccagg atgggcacca cccgtttaag agctaagctg gaaacagcat agcaagttta
      481 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctcg
      541 agtactagga tccattaggc ggccgccctg cagggtggat aaccgtatta ccgccatgca
      601 tgtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      661 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      721 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      781 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
      841 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
      901 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
      961 tcgaggcctt gcgcttaagg agccccttc
//
