LOCUS       sequence-169214-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of shRNAs, compatible with double-shRNA 
            expression, SFFV promoter, mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Kampmann M, Horlbeck MA, Chen Y, Tsai JC, Bassik MC, Gilbert LA, 
            Villalta JE, Kwon SC, Chang H, Kim VN, Weissman JS
  TITLE     Next-generation libraries for robust RNA interference-based 
            genome-wide screens.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3384-91. doi: 
            10.1073/pnas.1508821112. Epub 2015 Jun 15.
  PUBMED    26080438
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1508821112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-06-30- 30"; volume: "112"; issue: "26"; pages: 
            "E3384-91"
COMMENT     Sequence Label: pMK1221
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    569..686
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gactggcgcg ccgagtgagg ggttgtgagc tcttttatag agctcgggaa gcagaagcgc
       61 gcgaacagaa gcgagaagca ggctgattgg ttaattcaaa taaggcgcag ggtcatttca
      121 ggtccttggg ggagcctgga aacatctgat gggtcttaag aaactgctga gggttgggcc
      181 atatctgggg accatctgtt cttggcctcg ggccggggcc gaaactgcgg tgaccatctg
      241 ttcttggccc cgggccgggg ccgaaactgc tcaccgcaga tatcctgttt ggcccaacgt
      301 tagctatttt catgtacccg cccttgatct gaacttctct attcttggtt tggtattttt
      361 ccatgccttg caaaatggcg ttactgcagc tagcttgcca aacctacagg tggggtcttt
      421 cattcccccc tttttctgga gactaaataa aatcttttat tttatcgggg ctgcaggaat
      481 gcatggcggt aatacggtta tccacgcggc cctagagcgg gcccggtact aaccaaactg
      541 gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta
      601 attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc
      661 ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt ctgcagaatt
      721 ggcgcacgca gtgccgatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca
      781 ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga
      841 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
      901 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccaca
//
