LOCUS       sequence-169232-        6009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing XPO1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_XPO1_WT
FEATURES             Location/Qualifiers
     source          1..6009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3888..3987)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4004..4023)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4005..4023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4028..4044)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4028..4044)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4475..5266
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5359..5947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5848..5867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgccagcaa ttatgacaat gttagcagac catgcagctc gtcagctgct
      721 tgatttcagc caaaaactgg atatcaactt attagataat gtggtgaatt gcttatacca
      781 tggagaagga gcccagcaaa gaatggctca agaagtactg acacatttaa aggagcatcc
      841 tgatgcttgg acaagagtcg acacaatttt ggaattttct cagaatatga atacgaaata
      901 ctatggacta caaattttgg aaaatgtgat aaaaacaagg tggaagattc ttccaaggaa
      961 ccagtgcgaa ggaataaaaa aatacgttgt tggcctcatt atcaagacgt catctgaccc
     1021 aacttgtgta gagaaagaaa aggtgtatat cggaaaatta aatatgatcc ttgttcagat
     1081 actgaaacaa gaatggccca aacattggcc aacttttatc agtgatattg ttggagcaag
     1141 taggaccagc gaaagtctct gtcaaaataa tatggtgatt cttaaactct tgagtgaaga
     1201 agtatttgat ttctctagtg gacagataac ccaagtcaaa tctaagcatt taaaagacag
     1261 catgtgcaat gaattctcac agatatttca actgtgtcag tttgtaatgg aaaattctca
     1321 aaatgctcca cttgtacatg caaccttgga aacattgctc agatttctga actggattcc
     1381 cctgggatat atttttgaga ccaaattaat cagcacattg atttataagt tcctgaatgt
     1441 tccaatgttt cgaaatgtct ctctgaagtg cctcactgag attgctggtg tgagtgtaag
     1501 ccaatatgaa gaacaatttg taacactatt tactctgaca atgatgcaac taaagcagat
     1561 gcttccttta aataccaata ttcgacttgc gtactcaaat ggaaaagatg atgaacagaa
     1621 cttcattcaa aatctcagtt tgtttctctg cacctttctt aaggaacatg atcaacttat
     1681 agaaaaaaga ttaaatctca gggaaactct tatggaggcc cttcattata tgttgttggt
     1741 atctgaagta gaagaaactg aaatctttaa aatttgtctt gaatactgga atcatttggc
     1801 tgctgaactc tatagagaga gtccattctc tacatctgcc tctccgttgc tttctggaag
     1861 tcaacatttt gatgttcctc ccaggagaca gctatatttg cccatgttat tcaaggtccg
     1921 tttattaatg gttagtcgaa tggctaaacc agaggaagta ttggttgtag agaatgatca
     1981 aggagaagtt gtgagagaat tcatgaagga tacagattcc ataaatttgt ataagaatat
     2041 gagggaaaca ttggtttatc ttactcatct ggattatgta gatacagaaa gaataatgac
     2101 agagaagctt cacaatcaag tgaatggtac agagtggtca tggaaaaatt tgaatacatt
     2161 gtgttgggca ataggctcca ttagtggagc aatgcatgaa gaggacgaaa aacgatttct
     2221 tgttactgtt ataaaggatc tattaggatt atgtgaacag aaaagaggca aagataataa
     2281 agctattatt gcatcaaata tcatgtacat agtaggtcaa tacccacgtt ttttgagagc
     2341 tcactggaaa tttctgaaga ctgtagttaa caagctgttc gaattcatgc atgagaccca
     2401 tgatggagtc caggatatgg cttgtgatac tttcattaaa atagcccaaa aatgccgcag
     2461 gcatttcgtt caggttcagg ttggagaagt gatgccattt attgatgaaa ttttgaacaa
     2521 cattaacact attatttgtg atcttcagcc tcaacaggtt catacgtttt atgaagctgt
     2581 ggggtacatg attggtgcac aaacagatca aacagtacaa gaacacttga tagaaaagta
     2641 catgttactc cctaatcaag tgtgggatag tataatccag caggcaacca aaaatgtgga
     2701 tatactgaaa gatcctgaaa cagtcaagca gcttggtagc attttgaaaa caaatgtgag
     2761 agcctgcaaa gctgttggac acccctttgt aattcagctt ggaagaattt atttagatat
     2821 gcttaatgta tacaagtgcc tcagtgaaaa tatttctgca gctatccaag ctaatggtga
     2881 aatggttaca aagcaaccat tgattagaag tatgcgaact gtaaaaaggg aaactttaaa
     2941 gttaatatct ggttgggtga gccgatccaa tgatccacag atggtcgctg aaaattttgt
     3001 tccccctctg ttggatgcag ttctcattga ttatcagaga aatgtcccag ctgctagaga
     3061 accagaagtg cttagtacta tggccataat tgtcaacaag ttagggggac atataacagc
     3121 tgaaatacct caaatatttg atgctgtttt tgaatgcaca ttgaatatga taaataagga
     3181 ctttgaagaa tatcctgaac atagaacgaa ctttttctta ctacttcagg ctgtcaattc
     3241 tcattgtttc ccagcattcc ttgctattcc acctacacag tttaaacttg ttttggattc
     3301 catcatttgg gctttcaaac atactatgag gaatgtcgca gatacgggct tacagatact
     3361 ttttacactc ttacaaaatg ttgcacaaga agaagctgca gctcagagtt tttatcaaac
     3421 ttatttttgt gatattctcc agcatatctt ttctgttgtg acagacactt cacatactgc
     3481 tggtttaaca atgcatgcat caattcttgc atatatgttt aatttggttg aagaaggaaa
     3541 aataagtaca tcattaaatc ctggaaatcc agttaacaac caaatctttc ttcaggaata
     3601 tgtggctaat ctccttaagt cggccttccc tcacctacaa gatgctcaag taaagctctt
     3661 tgtgacaggg cttttcagct taaatcaaga tattcctgct ttcaaggaac atttaagaga
     3721 tttcctagtt caaataaagg aatttgcagg tgaagacact tctgatttgt ttttggaaga
     3781 gagagaaata gccctacggc aggctgatga agagaaacat aaacgtcaaa tgtctgtccc
     3841 tggcatcttt aatccacatg agattccaga agaaatgtgt gattgattgc caactttctt
     3901 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     3961 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     4021 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     4081 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     4141 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     4201 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     4261 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     4321 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     4381 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     4441 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     4501 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     4561 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     4621 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     4681 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     4741 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     4801 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     4861 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     4921 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     4981 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     5041 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     5101 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     5161 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     5221 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     5281 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     5341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5461 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5581 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5701 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5761 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5821 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5881 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5941 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6001 tcacatgtt
//
