LOCUS       sequence-169243-        3981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DCAF4L2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_DCAF4L2_WT
FEATURES             Location/Qualifiers
     source          1..3981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1860..1959)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1976..1995)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1977..1995)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2000..2016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2000..2016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2447..3238
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3331..3919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3820..3839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggagagca aaagaccgcg actgctcgag gaagcagaca agcagaaaaa
      721 gacagtcaga gtgggactca atgcaccttc catgctacga aagaaccagc taggtttcct
      781 cagattcgcc aactattgcc gtatagctcg cgagctgcgt gtaagctgca tgcagaggaa
      841 aaaggtccag attcatagct gggatccctc ctctttggca agcgaccgat ttaaccgcat
      901 actggcgaat accaacactg accagctctt cacagtgaac caagtcgaag ctggaggctc
      961 caagtacggc atcatcacca tgcgaggcct gacgacccct gagctccggg tatacccgca
     1021 caaaaccctc tacgtcccta atcggaaggt gaattctatg tgctgggcct cactgaatca
     1081 cttggattcc caccttctgc tgtgcttcgt gggacttgca gatactccaa gctgtgccgt
     1141 gctgctccca gcgtcgctgt tcataggtag cttcccagga atgcgtcggc ctggcatgct
     1201 ttgcagtttc cagatccctg atgcctggtc ctgtgcctgg tccctgagca tccacgcgta
     1261 tcactctttc agtacaggct tgtctcagca ggtcctgttg accaacgtgg tgacgggaca
     1321 ccagcagtca tttgggacta gcagtgatgt cttggcccag cagtttgcaa tcatgactcc
     1381 tttgctgttt aatggctgtc gctctgggga gatctttggc attgatctgc gctgtggaaa
     1441 tcaaggcagc gggtggaagg ccatttgcct gtcccatgat tcagcagtga cttctctgca
     1501 aatcctccaa gatggccaat tcctggtgtc atcagacatg actggaacta tcaagctgtg
     1561 ggacttgagg gccactaaat gtgtaacaca gtacgaaggt catgtgaata actccgccta
     1621 cctacccgtg catgtgaacg aagaagaagg agtcgtggcg gccgtgggcc aggactgcta
     1681 cacgagaatc tggagcctcc gtcatggcca cctgctcaca accataccct ccccataccc
     1741 cgcctcggag aacgacattc ccagtgtggc cttctcttct cgcctcgggg gcttccgagg
     1801 agcaccaggg ctgctcatgg ctgtccggga ggacctttat tgtttctcct acggttgatt
     1861 gccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     1921 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     1981 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     2041 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca
     2101 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     2161 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     2221 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     2281 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     2341 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     2401 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     2461 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     2521 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     2581 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     2641 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     2701 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     2761 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     2821 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     2881 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     2941 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     3001 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     3061 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     3121 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     3181 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     3241 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     3301 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3361 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3421 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3481 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3541 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3601 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3661 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3721 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3781 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3841 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3901 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3961 ctggcctttt gctcacatgt t
//
