LOCUS       sequence-169244-        4158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TMPO , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TMPO_WT
FEATURES             Location/Qualifiers
     source          1..4158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2037..2136)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2153..2172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2154..2172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2177..2193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2177..2193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2624..3415
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3508..4096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3997..4016
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgccggagt tcctggaaga cccctcggtc ctgacaaaag acaagttgaa
      721 gagtgagttg gtcgccaaca atgtgacgct gccggccggg gagcagcgca aagacgtgta
      781 cgtccagctc tacctgcagc acctcacggc tcgcaaccgg ccgccgctcc ccgccggcac
      841 caacagcaag gggcccccgg acttctccag tgacgaagag cgcgagccca ccccggtcct
      901 cggctctggg gccgccgccg cgggccggag ccgagcagcc gtcggcagga aagccacaaa
      961 aaaaactgat aaacccagac aagaagataa agatgatcta gatgtaacag agctcactaa
     1021 tgaagatctt ttggatcagc ttgtgaaata cggagtgaat cctggtccta ttgtgggaac
     1081 aaccaggaag ctatatgaga aaaagctttt gaaactgagg gaacaaggaa cagaatcaag
     1141 atcttctact cctctgccaa caatttcttc ttcagcagaa aatacaaggc agaatggaag
     1201 taatgattct gacagataca gtgacaatga agaagactct aaaatagagc tcaagcttga
     1261 gaagagagaa ccactaaagg gcagagcaaa gactccagta acactcaagc aaagaagagt
     1321 tgagcacaat cagagctatt ctcaagctgg aataactgag actgaatgga caagtggatc
     1381 ttcaaaaggc ggacctctgc aggcattaac tagggaatct acaagagggt caagaagaac
     1441 tccaaggaaa agggtggaaa cttcagaaca ttttcgtata gatggtccag taatttcaga
     1501 gagtactccc atagctgaaa ctataatggc ttcaagcaac gaatccttag ttgtcaatag
     1561 ggtgactgga aatttcaagc atgcatctcc tattctgcca atcactgaat tctcagacat
     1621 acccagaaga gcaccaaaga aaccattgac aagagctgaa gtgggagaaa aaacagagga
     1681 aagaagagta gaaagggata ttcttaagga aatgttcccc tatgaagcat ctacaccaac
     1741 aggaattagt gctagttgcc gcagaccaat caaaggggct gcaggccggc cattagaact
     1801 cagtgatttc aggatggagg agtctttttc atctaaatat gttcctaagt atgttccctt
     1861 ggcagatgtc aagtcagaaa agacaaaaaa gggacgctcc attcccgtat ggataaaaat
     1921 tttgctgttt gttgttgtgg cagttttttt gtttttggtc tatcaagcta tggaaaccaa
     1981 ccaagtaaat cccttctcta attttcttca tgttgaccct agaaaatcca actgattgcc
     2041 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2101 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2161 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2221 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     2281 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     2341 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     2401 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     2461 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     2521 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2581 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     2641 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2701 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2761 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2821 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2881 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2941 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     3001 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     3061 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3121 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3181 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3241 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3301 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3361 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     3421 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3481 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3541 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3601 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3661 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3721 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3781 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3841 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3901 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3961 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4021 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4081 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4141 gccttttgct cacatgtt
//
