LOCUS       sequence-169246-        4698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing POT1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_POT1_WT
FEATURES             Location/Qualifiers
     source          1..4698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2577..2676)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2693..2712)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2694..2712)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2717..2733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2717..2733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3164..3955
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4048..4636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4537..4556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgtctttgg ttccagcaac aaattatata tatacacccc tgaatcaact
      721 taagggtggt acaattgtca atgtctatgg tgttgtgaag ttctttaagc ccccatatct
      781 aagcaaagga actgattatt gctcagttgt aactattgtg gaccagacaa atgtaaaact
      841 aacttgcctg ctctttagtg gaaactatga agcccttcca ataatttata aaaatggaga
      901 tattgttcgc tttcacaggc tgaagattca agtatataaa aaggagactc agggtatcac
      961 cagctctggc tttgcatctt tgacgtttga gggaactttg ggagccccta tcatacctcg
     1021 cacttcaagc aagtatttta acttcactac tgaggaccac aaaatggtag aagccttacg
     1081 tgtttgggca tctactcata tgtcaccgtc ttggacatta ctaaaattgt gtgatgttca
     1141 gccaatgcag tattttgacc tgacttgtca gctcttgggc aaagcagaag tggacggagc
     1201 atcatttctt ctaaaggtat gggatggcac caggacacca tttccatctt ggagagtctt
     1261 aatacaagac cttgttcttg aaggtgattt aagtcacatc catcggctac aaaatctgac
     1321 aatagacatt ttagtctacg ataaccatgt tcatgtggca agatctctga aggttggaag
     1381 ctttcttaga atctatagcc ttcataccaa acttcaatca atgaattcag agaatcagac
     1441 aatgttaagt ttagagtttc atcttcatgg aggtaccagt tacggtcggg gaatcagggt
     1501 cttgccagaa agtaactctg atgtggatca actgaaaaag gatttagaat ctgcaaattt
     1561 gacagccaat cagcattcag atgttatctg tcaatcagaa cctgacgaca gctttccaag
     1621 ctctggatca gtatcattat acgaggtaga aagatgtcaa cagctatctg ctacaatact
     1681 tacagatcat cagtatttgg agaggacacc actatgtgcc attttgaaac aaaaagctcc
     1741 tcaacaatac cgcatccgag caaaattgag gtcatataag cccagaagac tatttcagtc
     1801 tgttaaactt cattgcccta aatgtcattt gctgcaagaa gttccacatg agggcgattt
     1861 ggatataatt tttcaggatg gtgcaactaa aaccccagat gtcaagctac aaaatacatc
     1921 attatatgat tcaaaaatct ggaccactaa aaatcaaaaa ggacgaaaag tagcagttca
     1981 ttttgtgaaa aataatggta ttctcccgct ttcaaatgaa tgtctacttt tgatagaagg
     2041 aggtacactc agtgaaattt gcaaactctc gaacaagttt aatagtgtaa ttcctgtgag
     2101 atctggccac gaagacctgg aacttttgga cctttcagca ccatttctta tacaaggaac
     2161 aatacatcac tatggatgta aacagtgttc tagtttgaga tccatacaaa atctaaattc
     2221 cctggttgat aaaacatcgt ggattccttc ttctgtggca gaagcactgg gtattgtacc
     2281 cctccaatat gtgtttgtta tgacctttac acttgatgat ggaacaggag tactagaagc
     2341 ctatctcatg gattctgaca aattcttcca gattccagca tcagaagttc tgatggatga
     2401 tgaccttcag aaaagtgtgg atatgatcat ggatatgttt tgtcctccag gaataaaaat
     2461 tgatgcatat ccgtggttgg aatgcttcat caagtcatac aatgtcacaa atggaacaga
     2521 taatcaaatt tgctatcaga tttttgacac cacagttgca gaagatgtaa tctgattgcc
     2581 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2641 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2701 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2761 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     2821 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     2881 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     2941 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     3001 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     3061 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     3121 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     3181 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     3241 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     3301 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     3361 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     3421 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     3481 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     3541 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     3601 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3661 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3721 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3781 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3841 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3901 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     3961 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     4021 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4081 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4141 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4201 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4261 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4321 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4381 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4441 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4501 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4561 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4621 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4681 gccttttgct cacatgtt
//
