LOCUS       sequence-169247-        4122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TUBB, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TUBB_WT
FEATURES             Location/Qualifiers
     source          1..4122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      42..630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     531..550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     784..801
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(960..987)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1079..1165)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1214..1236
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1228..1245
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1229..1245
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1261..1360
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2693..2792)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2809..2828)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2810..2828)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2833..2849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2833..2849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3280..4071
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
       61 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      121 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      181 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      241 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      301 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      361 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      421 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      481 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      541 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      601 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      661 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      721 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
      781 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
      841 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      901 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
      961 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     1021 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     1081 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     1141 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     1201 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     1261 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     1321 tgctttttta taatgccaac tttgtacaaa aaagttggca tgagggaaat cgtgcacatc
     1381 caggctggtc agtgtggcaa ccagatcggt gccaagttct gggaggtgat cagtgatgaa
     1441 catggcatcg accccaccgg cacctaccac ggggacagcg acctgcagct ggaccgcatc
     1501 tctgtgtact acaatgaagc cacaggtggc aaatatgttc ctcgtgccat cctggtggat
     1561 ctagaacctg ggaccatgga ctctgttcgc tcaggtcctt ttggccagat ctttagacca
     1621 gacaactttg tatttggtca gtctggggca ggtaacaact gggccaaagg ccactacaca
     1681 gagggcgccg agctggttga ttctgtcctg gatgtggtac ggaaggaggc agagagctgt
     1741 gactgcctgc agggcttcca gctgacccac tcactgggcg ggggcacagg ctctggaatg
     1801 ggcactctcc ttatcagcaa gatccgagaa gaataccctg atcgcatcat gaataccttc
     1861 agtgtggtgc cttcacccaa agtgtctgac accgtggtcg agccctacaa tgccaccctc
     1921 tccgtccatc agttggtaga gaatactgat gagacctatt gcattgacaa cgaggccctc
     1981 tatgatatct gcttccgcac tctgaagctg accacaccaa cctacgggga tctgaaccac
     2041 cttgtctcag ccaccatgag tggtgtcacc acctgcctcc gtttccctgg ccagctcaat
     2101 gctgacctcc gcaagttggc agtcaacatg gtccccttcc cacgtctcca tttctttatg
     2161 cctggctttg cccctctcac cagccgtgga agccagcagt atcgagctct cacagtgccg
     2221 gaactcaccc agcaggtctt cgatgccaag aacatgatgg ctgcctgtga cccccgccac
     2281 ggccgatacc tcaccgtggc tgctgtcttc cgtggtcgga tgtccatgaa ggaggtcgat
     2341 gagcagatgc ttaacgtgca gaacaagaac agcagctact ttgtggaatg gatccccaac
     2401 aatgtcaaga cagccgtctg tgacatccca cctcgtggcc tcaagatggc agtcaccttc
     2461 attggcaata gcacagccat ccaggagctc ttcaagcgca tctcggagca gttcactgcc
     2521 atgttccgcc ggaaggcctt cctccactgg tacacaggcg agggcatgga cgagatggag
     2581 ttcaccgagg ctgagagcaa catgaacgac ctcgtctctg agtatcagca gtaccaggat
     2641 gccaccgcag aagaggagga ggatttcggt gaggaggccg aagaggaggc ctacccaact
     2701 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     2761 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     2821 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     2881 tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga
     2941 aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg
     3001 aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     3061 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     3121 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     3181 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     3241 acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc
     3301 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     3361 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     3421 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     3481 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     3541 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     3601 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     3661 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     3721 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     3781 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     3841 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     3901 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     3961 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     4021 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc
     4081 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tc
//
