LOCUS       sequence-169248-        5238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GPR156 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GPR156_WT
FEATURES             Location/Qualifiers
     source          1..5238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3117..3216)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3233..3252)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3234..3252)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3257..3273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3257..3273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3704..4495
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4588..5176
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5077..5096
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggagcctg aaataaactg ctctgaattg tgtgacagtt ttcctggcca
      721 ggagctggat cggagacccc ttcatgatct ctgcaagaca acaattacat cttcccacca
      781 cagcagtaag accatctctt cattatctcc tgtcctcttg ggtattgttt ggacttttct
      841 cagctgtgga cttctgctga tacttttctt tcttgccttt acaattcact gcaggaagaa
      901 caggattgtg aagatgtcca gtcccaatct gaacattgtg accttactgg gcagttgtct
      961 cacttacagt agcgcttacc tctttgggat tcaggatgtt ttagtgggga gctcaatgga
     1021 aactctcatt cagacaagac tgtccatgct gtgcattggg acctcccttg tgtttggccc
     1081 cattctggga aagagctggc gactctacaa ggtgtttacc caaagggtcc cggacaagag
     1141 agtgattatc aaagacctgc agttgctggg gttggtggca gccctgttga tggctgatgt
     1201 gatcctgctc atgacgtggg tgctgactga tcccatccag tgcctccaga ttctcagtgt
     1261 cagtatgacg gtgacaggga aagacgtgtc ctgcacttcg accagcaccc acttctgtgc
     1321 ttcccggtat tccgatgttt ggattgctct catttgggga tgcaagggtc tgctcctgct
     1381 gtatggtgcc tacctggctg gcctgactgg ccatgtcagc tcccctcctg tgaatcagtc
     1441 cttaaccatc atggtggggg tcaacctcct tgtactggct gctgggctgc tttttgtagt
     1501 caccagatac ttgcattcct ggcccaacct ggtctttgga ctcacatctg gagggatctt
     1561 tgtttgtaca actacaatca actgcttcat cttcattccc cagctgaagc aatggaaggc
     1621 atttgaagag gaaaaccaaa caatcagacg catggccaaa tatttcagca ctcccaacaa
     1681 aagcttccat acccagtatg gtgaggagga gaactgccac ccgaggggag agaaaagctc
     1741 catggagagg ctcctcacag aaaaaaatgc tgtgattgaa agcctgcagg aacaagtaaa
     1801 caacgccaaa gagaagattg tgaggctgat gtcagctgag tgcacctatg acctcccaga
     1861 gggggctgcc ccacctgcct cttccccgaa caaggacgtc caggcggtag cctcggtcca
     1921 caccctggca gctgctcagg ggccttcggg tcacctctct gactttcaga atgatcctgg
     1981 catggctgcc cgggattccc agtgcacttc agggccctcc tcatatgcac aaagccttga
     2041 ggggcctggg aaggactcca gcttctcccc agggaaggag gagaagatat ctgactcaaa
     2101 agacttttct gatcatttag actcaggttg tagccagaag ccatggactg agcaaagcct
     2161 gggtccagaa agaggagacc aagtccccat gaaccccagc cagagtctcc taccagatag
     2221 aggcggctca gatccccaga gacagaggca tctggagaac tcagaggagc ccccagagcg
     2281 gcggtcacgg gttagttcag taatcaggga gaaacttcag gaggtcttac aagatctggg
     2341 cctgggccct gaggcttccc tctccaccgc cccctcttgt catcagcaaa cctggaagaa
     2401 cagtgctgcc ttcagccccc aaaagatgcc cctctccaag gagctgggct ttagccctta
     2461 catggtgagg agaaggcggg cagctcagcg ggcccgctca cactttcctg gctctgcacc
     2521 ctcatctgtg gggcatcggg caaacaggac tgttcctggg gcacacagca ggctacatgt
     2581 gcagaatggg gacagcccca gcctggcccc acaaactact gattccagag tacgaagacc
     2641 ttcttccagg aagccttcac taccttcaga tcctcaagac agaccaggta ccctggaggg
     2701 cagcaaacaa agccagacag agcccgaggg ggctagaggg agcaaagcag cctttcttcg
     2761 ccagccttct ggttctggcc gggccccaag tcctgctgcc ccatgcctct ccaaagcctc
     2821 acctgacttg cctgaacagt ggcagctgtg gcccccagtg ccctcaggct gtgcctccct
     2881 gtcttctcaa cacagctatt ttgatactga gtccagcagc tcagatgagt tcttctgccg
     2941 ctgccaccgg ccctactgtg aaatctgctt ccagagctct tctgactcta gtgacagtgg
     3001 cacatcagac actgaccctg agcctactgg ggggctggct tcctgggaaa agctgtgggc
     3061 ccgctccaag cctattgtga acttcaaaga tgacttgaaa cccacgctgg tgtgattgcc
     3121 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     3181 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     3241 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     3301 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     3361 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     3421 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     3481 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     3541 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     3601 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     3661 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     3721 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     3781 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     3841 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     3901 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     3961 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     4021 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     4081 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     4141 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     4201 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     4261 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     4321 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     4381 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     4441 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     4501 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     4561 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4621 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4681 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4741 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4801 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4861 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4921 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4981 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5041 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5101 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5161 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5221 gccttttgct cacatgtt
//
