LOCUS       sequence-169250-        4302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CYP3A5 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CYP3A5_WT
FEATURES             Location/Qualifiers
     source          1..4302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2181..2280)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2297..2316)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2298..2316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2321..2337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2321..2337)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2768..3559
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3652..4240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4141..4160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggacctca tcccaaattt ggcggtggaa acctggcttc tcctggctgt
      721 cagcctggtg ctcctctatc tatatgggac ccgtacacat ggacttttta agagactggg
      781 aattccaggg cccacacctc tgcctttgtt gggaaatgtt ttgtcctatc gtcagggtct
      841 ctggaaattt gacacagagt gctataaaaa gtatggaaaa atgtggggaa cgtatgaagg
      901 tcaactccct gtgctggcca tcacagatcc cgacgtgatc agaacagtgc tagtgaaaga
      961 atgttattct gtcttcacaa atcgaaggtc tttaggccca gtgggattta tgaaaagtgc
     1021 catctcttta gctgaggatg aagaatggaa gagaatacgg tcattgctgt ctccaacctt
     1081 caccagcgga aaactcaagg agatgttccc catcattgcc cagtatggag atgtattggt
     1141 gagaaacttg aggcgggaag cagagaaagg caagcctgtc accttgaaag acatctttgg
     1201 ggcctacagc atggatgtga ttactggcac atcatttgga gtgaacatcg actctctcaa
     1261 caatccacaa gacccctttg tggagagcac taagaagttc ctaaaatttg gtttcttaga
     1321 tccattattt ctctcaataa tactctttcc attccttacc ccagtttttg aagcattaaa
     1381 tgtctctctg tttccaaaag ataccataaa ttttttaagt aaatctgtaa acagaatgaa
     1441 gaaaagtcgc ctcaacgaca aacaaaagca ccgactagat ttccttcagc tgatgattga
     1501 ctcccagaat tcgaaagaaa ctgagtccca caaagctctg tctgatctgg agctcgcagc
     1561 ccagtcaata atcttcattt ttgctggcta tgaaaccacc agcagtgttc tttccttcac
     1621 tttatatgaa ctggccactc accctgatgt ccagcagaaa ctgcaaaagg agattgatgc
     1681 agttttgccc aataaggcac cacctaccta tgatgccgtg gtacagatgg agtaccttga
     1741 catggtggtg aatgaaacac tcagattatt cccagttgct attagacttg agaggacttg
     1801 caagaaagat gttgaaatca atggggtatt cattcccaaa gggtcaatgg tggtgattcc
     1861 aacttatgct cttcaccatg acccaaagta ctggacagag cctgaggagt tccgccctga
     1921 aaggttcagt aagaagaagg acagcataga tccttacata tacacaccct ttggaactgg
     1981 acccagaaac tgcattggca tgaggtttgc tctcatgaac atgaaacttg ctctaatcag
     2041 agtccttcag aacttctcct tcaaaccttg taaagaaaca cagatcccct tgaaattaga
     2101 cacgcaagga cttcttcaac cagaaaaacc cattgttcta aaggtggatt caagagatgg
     2161 aaccctaagt ggagaatgat tgccaacttt cttgtacaaa gttggcatta taagaaagca
     2221 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2281 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2341 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2401 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2461 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2521 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2581 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2641 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2701 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2761 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2821 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2881 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     2941 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3001 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     3061 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3121 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3181 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3241 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3301 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3361 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3421 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3481 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3541 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3601 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3661 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3721 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3781 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3841 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3901 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3961 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4021 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4081 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4141 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4201 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4261 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
