LOCUS       sequence-169273-        3090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SPANXN4, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SPANXN4_WT
FEATURES             Location/Qualifiers
     source          1..3090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      33..621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     522..541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     775..792
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(951..978)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1070..1156)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1205..1227
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1219..1236
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1220..1236
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1252..1351
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1652..1751)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1768..1787)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1769..1787)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1792..1808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1792..1808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2239..3030
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
       61 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      121 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      181 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      241 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      301 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      361 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      421 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      481 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      541 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      601 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      661 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      721 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
      781 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
      841 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      901 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
      961 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     1021 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     1081 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     1141 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     1201 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg
     1261 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
     1321 ataatgccaa ctttgtacaa aaaagttggc accatggaag agccaacttc cagcaccaac
     1381 gagaataaaa tgaagagccc ctgtgaatct aacaaaagaa aagttgacaa gaagaagaag
     1441 aatctgcaca gagcctcagc ccctgaacag agtttgaaag agacagaaaa agcaaaatat
     1501 ccaacattag tgttttactg caggaagaat aagaaaagaa attcaaatca actggagaat
     1561 aaccagccta cagagagctc cactgatcca atcaaagaga aaggagacct agacatatct
     1621 gcaggatctc cacaggatgg tgggcagaat ttgccaactt tcttgtacaa agttggcatt
     1681 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1741 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     1801 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1861 aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta
     1921 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca
     1981 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     2041 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     2101 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     2161 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     2221 aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     2281 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     2341 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     2401 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     2461 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     2521 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     2581 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     2641 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     2701 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     2761 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     2821 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     2881 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     2941 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     3001 ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt
     3061 aacgtgagtt acgcgtcgtt ccactgagcg
//
