LOCUS       sequence-169274-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSINAPS; single molecule translation reporter containing a FLAG tag,
            Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Wu B, Eliscovich C, Yoon YJ, Singer RH
  TITLE     Translation dynamics of single mRNAs in live cells and neurons.
  JOURNAL   Science. 2016 Jun 17;352(6292):1430-5. doi: 10.1126/science.aaf1084.
            Epub 2016 May 5.
  PUBMED    27313041
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaf1084"; journalName: "Science"; date: 
            "2016-06-17- 17"; volume: "352"; issue: "6292"; pages: "1430-5"
COMMENT     Sequence Label: 
            pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             75..98
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             138..194
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             210..266
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             282..338
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             354..410
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             426..482
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             498..554
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             570..626
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             642..698
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             714..770
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             786..842
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             858..914
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
       61 cggccgccac catggattac aaggatgacg acgataaggg cggaccgggt ggatctggag
      121 gtggaggttc tggaggagaa gaacttttga gcaagaatta tcatcttgag aacgaagtgg
      181 ctcgtcttaa gaaaggttct ggcagtggag aagaactgct ttcaaagaat taccacctgg
      241 aaaatgaggt agctagactg aaaaagggga gcggaagtgg ggaggagttg ctgagcaaaa
      301 attatcattt ggagaacgaa gtagcacgac taaagaaagg gtccggatcg ggtgaggagt
      361 tactctcgaa aaattatcat ctcgaaaacg aagtggctcg gctaaaaaag ggcagtggtt
      421 ctggagaaga gctattatct aaaaactacc acctcgaaaa tgaggtggca cgcttaaaaa
      481 agggaagtgg cagtggtgaa gagctactat ccaagaatta tcatcttgag aacgaggtag
      541 cgcgtttgaa gaagggttcc ggctcaggag aggaactgct ctcgaagaac tatcatcttg
      601 aaaatgaggt cgctcgatta aaaaagggat cgggcagtgg tgaggaacta ctttcaaaga
      661 attaccacct cgaaaacgaa gtagctcgat taaagaaagg ttcagggtcg ggtgaagaat
      721 tactgagtaa aaattatcat ctggaaaatg aggtagcgag actaaaaaag gggagtggtt
      781 ctggcgaaga gttgctatcg aaaaattatc atcttgagaa cgaagttgct aggctcaaaa
      841 agggctcagg ctcaggcgag gagttgctct cgaaaaacta ccacttggaa aatgaggtcg
      901 cgaggttgaa aaaggggagc gggtcgggcg aggagttatt gagcaaaaac tatcatttag
      961 agaacgaagt cgcgcgctta aa
//
