LOCUS       sequence-169279-        4179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3R3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3R3_WT
FEATURES             Location/Qualifiers
     source          1..4179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2058..2157)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2174..2193)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2175..2193)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2198..2214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2198..2214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2645..3436
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3529..4117
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4018..4037
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgtacaata cggtgtggag tatggaccgc gatgacgcag actggaggga
      721 ggtgatgatg ccctattcga cagaactgat attttatatt gaaatggatc ctccagctct
      781 tccaccaaag ccacctaagc caatgacttc agcagttcca aatggaatga aggacagttc
      841 tgtttctctt caggatgcag aatggtactg gggggatatt tcaagggagg aggtaaatga
      901 caaattgcgg gatatgccag atgggacctt cttggtccga gatgcctcaa caaaaatgca
      961 gggagattat actttgactt tgcggaaggg aggcaataat aagttaataa agatctatca
     1021 ccgggatggt aaatatggct tttctgatcc tctgacattt aattccgtgg tggagctcat
     1081 taaccactat caccatgaat ctcttgctca gtacaatccc aaacttgatg tgaagctgat
     1141 gtacccagtg tccagatacc aacaggatca gttggtaaaa gaagataata ttgatgcagt
     1201 aggtaaaaaa ctgcaagaat accactctca gtatcaggag aagagtaaag agtatgatag
     1261 gctgtatgaa gaatatacta gaacatccca ggaaatacag atgaagagga ctgcaataga
     1321 agcttttaat gaaacaatta aaatatttga agagcagtgt cacacacaag aacaacatag
     1381 caaagaatat attgagcgat ttcgcagaga ggggaatgaa aaggagattg aacgaattat
     1441 gatgaattat gataaattga aatcacgtct gggtgagatt catgatagca aaatgcgtct
     1501 agagcaggat ttgaagaatc aagctttgga caaccgagaa atagataaaa aaatgaatag
     1561 catcaaacct gacctgatcc agctgcgaaa gatccgagat caacaccttg tatggctcaa
     1621 tcacaaagga gtgagacaga aacgcctgaa tgtctggctg ggaattaaga atgaggatgc
     1681 tgctgagaac tattttatca atgaggaaga tgaaaacctg ccccattatg atgagaaaac
     1741 ctggtttgtt gaggatatca atcgagtaca agcagaggac ttgctttatg ggaaacctga
     1801 tggtgcattc ttaattcgtg agagtagcaa gaaaggatgc tatgcttgct ctgtggtggc
     1861 cgatggggaa gtgaagcact gtgtgatcta cagcactgct cggggctatg gctttgcaga
     1921 gccctacaac ctgtacagct ctctgaagga gctagtgctc cattaccagc agacatcctt
     1981 ggttcagcac aacgactccc tcaacgtcag gcttgcctac cctgttcatg cacagatgcc
     2041 ctcgctttgc agatgattgc caactttctt gtacaaagtt gtcattataa gaaagcattg
     2101 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2161 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2221 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2281 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     2341 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     2401 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     2461 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     2521 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2581 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     2641 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     2701 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     2761 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     2821 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     2881 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     2941 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     3001 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     3061 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     3121 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     3181 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3241 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3301 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3361 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     3421 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     3481 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3541 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3601 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3661 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3721 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3781 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3841 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3901 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3961 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4021 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4081 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4141 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
