LOCUS       sequence-169291-        3369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CD70, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CD70_WT
FEATURES             Location/Qualifiers
     source          1..3369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     525..544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     778..795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(954..981)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1073..1159)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1208..1230
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1222..1239
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1223..1239
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1255..1354
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1934..2033)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2050..2069)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2051..2069)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2074..2090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2074..2090)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2521..3312
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      181 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      241 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      301 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      361 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      421 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      481 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      541 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      601 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      661 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      721 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      781 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      841 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      901 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
      961 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     1021 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     1081 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     1141 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     1201 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
     1261 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
     1321 tttataatgc caactttgta caaaaaagtt ggcatgccgg aggagggttc gggctgctcg
     1381 gtgcggcgca ggccctatgg gtgcgtcctg cgggctgctt tggtcccatt ggtcgcgggc
     1441 ttggtgatct gcctcgtggt gtgcatccag cgcttcgcac aggctcagca gcagctgccg
     1501 ctcgagtcac ttgggtggga cgtagctgag ctgcagctga atcacacagg acctcagcag
     1561 gaccccaggc tatactggca ggggggccca gcactgggcc gctccttcct gcatggacca
     1621 gagctggaca aggggcagct acgtatccat cgtgatggca tctacatggt acacatccag
     1681 gtgacgctgg ccatctgttc ctccacgacg gcctccaggc accaccccac caccctggcc
     1741 gtgggaatct gctctcccgc ctcccgtagc atcagcctgc tgcgtctcag cttccaccaa
     1801 ggttgtacca ttgcctccca gcgcctgacg cccctggccc gaggggacac actctgcacc
     1861 aacctcactg ggacactttt gccttcccga aacactgatg agaccttctt tggagtgcag
     1921 tgggtgcgtc cctgcccaac tttcttgtac aaagttggca ttataagaaa gcattgctta
     1981 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     2041 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     2101 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
     2161 ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga
     2221 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
     2281 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     2341 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     2401 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     2461 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt
     2521 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     2581 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     2641 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     2701 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     2761 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     2821 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     2881 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     2941 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     3001 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     3061 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     3121 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     3181 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     3241 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     3301 gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3361 ttccactga
//
