LOCUS       sequence-169295-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene targeting vector for the mouse Rosa26 locus, including a CAG 
            promoter, loxP flanked stop cassette, GFP, for gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P,
            Rajewsky K, Kuhn R
  TITLE     Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in 
            C57BL/6 zygotes.
  JOURNAL   BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
  PUBMED    26772810
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-01-16"; volume: "16(1):4. doi"; pages: " 
            10.1186/s12896-016-0234-4"
COMMENT     Sequence Label: pR26 CAG/GFP Dest
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     104..127
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 aagctgggta ccgcggccgc gatcgccctg cagggcggcg gcgcctaccg gtggatgtgg
       61 aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag
      121 cgcatgctcc agactgcctt gggaaaagcg cctcccctac ccggtagaat tcgatatcaa
      181 gcttatcgat accgtcgacg gtatcgataa gctagatgtc ctgaaatatt tctatcagaa
      241 caaggtagta taaagctggt aggtatacaa aacgctagac tagtttctat ccctgaccct
      301 taatctgcta gtatatccgt aggaagttgc ttaagtgcca ctagtaccaa cagcctctgt
      361 tagagtgaac agtagatttt aatgaagggc caataaagac cacttaagac tactgactga
      421 attaaaaatc cacccagtgg agttagtatt ggttatagtt atataacaaa atatccaact
      481 tagccaggcg tggtggtgca cgcctttaat cccggcactc aggaggcaga ggcaggcgga
      541 tttctgagtt cgaggccagc ctggtctaca aagtgagttc caggacagcc agggctatac
      601 agagaaaccc tgtctcgaaa aaccaaaaaa aaaaaaaaaa aggaaaaaaa atatggcttg
      661 aaaacatgct attgttgtca aaaggagatc caaacaagta tttctttgaa caaaaattat
      721 gtagaaaaca gggtgataaa gaacaagggc tttttaaaaa aggccagcat tatttgagat
      781 atacaa
//
