LOCUS       sequence-169312-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cancer-associated ASXL1 truncation mutant in mammalian expression 
            vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Balasubramani A, Larjo A, Bassein JA, Chang X, Hastie RB, Togher SM,
            Lahdesmaki H, Rao A
  TITLE     Cancer-associated ASXL1 mutations may act as gain-of-function 
            mutations of the ASXL1-BAP1 complex.
  JOURNAL   Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307.
  PUBMED    26095772
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-06-22"; volume: "6:7307. doi"; pages: " 
            10.1038/ncomms8307"
COMMENT     Sequence Label: pCMV6-XL4 ASXL1 (p.G646 Wfs *12) 3x FLAG
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     181..197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(257..322)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 acttgcccct tgctccatac cacccccctc caccccataa tattatagaa ggacacctag
       61 tcagacaaaa tgatgcaact taattttatt aggacaaggc tggtgggcac tggagtggca
      121 acttccaggg ccaggagagg cactggggag gggtcacagg gatgccaccc gggatctgtt
      181 caggaaacag ctatgaccgc ggccgcaatc tagagtcgac aagcttgata tcggtaccag
      241 atctgaattc ttatcattta tcatcatcat ctttataatc gatgtcgtgg tccttgtagt
      301 cgccgtcgtg gtccttgtag tctcggtggc cccgccgcca cctccacccg ggccaccccc
      361 cccctccgat ggcagtggtg gccgcctctc tatggcagtg gtgacctctc gcccctcgga
      421 cctgcagagc acgggcttta atgtctgcga gggtcctggc gccagtccaa ccccggcagg
      481 aggactccgt ggtggggatg atccgggggc atatctggta agtgggctga cctttaacca
      541 cccagggtgg tttgatacgt gaaagttgaa tccggatggg cgggactttg ggctcctctt
      601 tagtgggctg tggcttttcg gtgtgaacac tttcaattgt gttacgaaag gactgacgat
      661 cttcaagccg gggcttcttt tcgggaaagg atgcagaggc cgcctgctca aaggatttcc
      721 tcttctgatc cttgggttcc tgatccacag tttcctgagg caggctagga atcctgtctg
      781 gagatgcaga ggcacgtgcc aagttgtctg gctcagcctg gatccgagaa gccacagact
      841 caactgggaa ctcgggaccc tcca
//
