LOCUS       sequence-169332-        3800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PSG2 ,part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PSG2_WT
FEATURES             Location/Qualifiers
     source          1..3800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1679..1778)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1795..1814)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1796..1814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1819..1835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1819..1835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2266..3057
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3150..3738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3639..3658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggggcccc tctcagcccc tccctgcaca gagcacatca aatggaaggg
      721 gctcctgctc acagcatcac ttttaaactt ctggaacctg cccaccactg cccaagtcac
      781 gattgaagcc cagccaccaa aagtttccga ggggaaggat gttcttctac ttgtccacaa
      841 tttgccccag aatcttactg gctacatctg gtacaaaggg caaatcaggg acctctacca
      901 ttacattaca tcatatgtag tagacggtca aataattata tatgggcctg catatagtgg
      961 acgagaaaca gcatattcca atgcatccct gctgatccag aatgtcaccc gggaggacgc
     1021 aggatcctac accttacaca tcataaagcg aggtgatggg actagaggag taactggata
     1081 tttcaccttc accttatacc tggagactcc caagccctcc atctccagca gcaacttaaa
     1141 ccccagggag gccatggaaa ctgtgatctt aacctgtgat cctgagactc cggacacaag
     1201 ctaccagtgg tggatgaatg gtcagagcct ccctatgact cacaggtttc agctgtccga
     1261 aaccaacagg accctctttc tatttggtgt cacaaagtat actgcaggac cctatgaatg
     1321 tgaaatacgg aactcaggga gtgccagccg cagtgaccca gtcaccctga atctcctcca
     1381 tggtccagac ctccccagaa ttcacccttc atacaccaat taccgttcag gagataacct
     1441 ctacttgtct tgcttcgcga actctaaccc accggcacag tattcttgga caattaatgg
     1501 gaagtttcag caatcaggac aaaatctgtt tatcccccaa attactacaa agcatagcgg
     1561 gctctatgtt tgctctgttc gtaactcagc cactggcgag gaaagctcca cgtcgttgac
     1621 agtcaaagtc tctgcttcta caagaatagg acttcttcct ctccttatcc aacatgattg
     1681 ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1741 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1801 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1861 ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag
     1921 gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa
     1981 cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     2041 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     2101 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     2161 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     2221 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg
     2281 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     2341 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     2401 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     2461 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     2521 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     2581 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     2641 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     2701 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     2761 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     2821 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     2881 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     2941 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     3001 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc
     3061 tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     3121 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3181 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt ttttgccgga tcaagagcta
     3241 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3301 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3361 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3421 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3481 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3541 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3601 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3661 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3721 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3781 tggccttttg ctcacatgtt
//
