LOCUS       sequence-169336-        4038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing IDH1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IDH1_WT
FEATURES             Location/Qualifiers
     source          1..4038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1917..2016)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2033..2052)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2034..2052)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2057..2073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2057..2073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2504..3295
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3388..3976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3877..3896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgtccaaaa aaatcagtgg cggttctgtg gtagagatgc aaggagatga
      721 aatgacacga atcatttggg aattgattaa agagaaactc atttttccct acgtggaatt
      781 ggatctacat agctatgatt taggcataga gaatcgtgat gccaccaacg accaagtcac
      841 caaggatgct gcagaagcta taaagaagca taatgttggc gtcaaatgtg ccactatcac
      901 tcctgatgag aagagggttg aggagttcaa gttgaaacaa atgtggaaat caccaaatgg
      961 caccatacga aatattctgg gtggcacggt cttcagagaa gccattatct gcaaaaatat
     1021 cccccggctt gtgagtggat gggtaaaacc tatcatcata ggtcgtcatg cttatgggga
     1081 tcaatacaga gcaactgatt ttgttgttcc tgggcctgga aaagtagaga taacctacac
     1141 accaagtgac ggaacccaaa aggtgacata cctggtacat aactttgaag aaggtggtgg
     1201 tgttgccatg gggatgtata atcaagataa gtcaattgaa gattttgcac acagttcctt
     1261 ccaaatggct ctgtctaagg gttggccttt gtatctgagc accaaaaaca ctattctgaa
     1321 gaaatatgat gggcgtttta aagacatctt tcaggagata tatgacaagc agtacaagtc
     1381 ccagtttgaa gctcaaaaga tctggtatga gcataggctc atcgacgaca tggtggccca
     1441 agctatgaaa tcagagggag gcttcatctg ggcctgtaaa aactatgatg gtgacgtgca
     1501 gtcggactct gtggcccaag ggtatggctc tctcggcatg atgaccagcg tgctggtttg
     1561 tccagatggc aagacagtag aagcagaggc tgcccacggg actgtaaccc gtcactaccg
     1621 catgtaccag aaaggacagg agacgtccac caatcccatt gcttccattt ttgcctggac
     1681 cagagggtta gcccacagag caaagcttga taacaataaa gagcttgcct tctttgcaaa
     1741 tgctttggaa gaagtctcta ttgagacaat tgaggctggc ttcatgacca aggacttggc
     1801 tgcttgcatt aaaggtttac ccaatgtgca acgttctgac tacttgaata catttgagtt
     1861 catggataaa cttggagaaa acttgaagat caaactagct caggccaaac tttaattgcc
     1921 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1981 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2041 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2101 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     2161 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     2221 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     2281 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     2341 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     2401 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2461 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     2521 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2581 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2641 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2701 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2761 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2821 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2881 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     2941 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3001 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3061 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3121 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3181 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3241 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     3301 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4021 gccttttgct cacatgtt
//
