LOCUS       sequence-169350-        2532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruitable Akt2(K14A) mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ebner M, Sinkovics B, Szczygiel M, Ribeiro DW, Yudushkin I
  TITLE     Localization of mTORC2 activity inside cells.
  JOURNAL   J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 
            10.1083/jcb.201610060.
  PUBMED    28143890
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060."
COMMENT     Sequence Label: C1-FKBP(F36V):mCherry-Akt2(K14A)
FEATURES             Location/Qualifiers
     source          1..2532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(18..20,21..341)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             366..1073
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(511..529)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     779..798
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tggcaagcta gcccaccatg ggagtgcagg tggagactat ctccccagga gacgggcgca
       61 ccttccccaa gcgcggccag acctgcgtgg tgcactacac cgggatgctt gaagatggaa
      121 agaaagttga ttcctcccgg gacagaaaca agccctttaa gtttatgcta ggcaagcagg
      181 aggtgatccg aggctgggaa gaaggggttg cccagatgag tgtgggtcag agagccaaac
      241 tgactatatc tccagattat gcctatggtg ccactgggca cccaggcatc atcccaccac
      301 atgccactct cgtcttcgat gtggagcttc taaaactgga aggtgctacg cgtctgcccg
      361 gttctatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
      421 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      481 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
      541 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
      601 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      661 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      721 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
      781 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
      841 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
      901 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
      961 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     1021 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagatgaatg
     1081 aggtgtctgt catcaaagaa ggctggctcc acgcacgtgg tgaatacatc aagacctgga
     1141 ggccacggta cttcctgctg aagagcgacg gctccttcat tgggtacaag gagaggcccg
     1201 aggcccctga tcagactcta ccccccttaa acaacttctc cgtagcagaa tgccagctga
     1261 tgaagaccga gaggccgcga cccaacacct ttgtcatacg ctgcctgcag tggaccacag
     1321 tcatcgagag gaccttccac gtggattctc cagacgagag ggaggagtgg atgcgggcca
     1381 tccagatggt cgccaacagc ctcaagcagc gggccccagg cgaggacccc atggactaca
     1441 agtgtggctc ccccagtgac tcctccacga ctgaggagat ggaagtggcg gtcagcaagg
     1501 cacgggctaa agtgaccatg aatgacttcg actatctcaa actccttggc aagggaacct
     1561 ttggcaaagt catcctggtg cgggagaagg ccactggccg ctactacgcc atgaagatcc
     1621 tgcggaagga agtcatcatt gccaaggatg aagtcgctca cacagtcacc gagagccggg
     1681 tcctccagaa caccaggcac ccgttcctca ctgcgctgaa gtatgccttc cagacccacg
     1741 accgcctgtg ctttgtgatg gagtatgcca acgggggtga gctgttcttc cacctgtccc
     1801 gggagcgtgt cttcacagag gagcgggccc ggttttatgg tgcagagatt gtctcggctc
     1861 ttgagtactt gcactcgcgg gacgtggtat accgcgacat caagctggaa aacctcatgc
     1921 tggacaaaga tggccacatc aagatcactg actttggcct ctgcaaagag ggcatcagtg
     1981 acggggccac catgaaaacc ttctgtggga ccccggagta cctggcgcct gaggtgctgg
     2041 aggacaatga ctatggccgg gccgtggact ggtgggggct gggtgtggtc atgtacgaga
     2101 tgatgtgcgg ccgcctgccc ttctacaacc aggaccacga gcgcctcttc gagctcatcc
     2161 tcatggaaga gatccgcttc ccgcgcacgc tcagccccga ggccaagtcc ctgcttgctg
     2221 ggctgcttaa gaaggacccc aagcagaggc ttggtggggg gcccagcgat gccaaggagg
     2281 tcatggagca caggttcttc ctcagcatca actggcagga cgtggtccag aagaagctcc
     2341 tgccaccctt caaacctcag gtcacgtccg aggtcgacac aaggtacttc gatgatgaat
     2401 ttaccgccca gtccatcaca atcacacccc ctgaccgcta tgacagcctg ggcttactgg
     2461 agctggacca gcggacccac ttcccccagt tctcctactc ggccagcatc cgcgagtgag
     2521 gatccaccgg tc
//
