LOCUS       sequence-169359-        7217 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon 
            CH, Novina CD
  TITLE     The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved 
            SRA1-like Region.
  JOURNAL   Cell Rep. 2016 May 31;15(9):2025-37. doi: 
            10.1016/j.celrep.2016.04.018. Epub 2016 May 19.
  PUBMED    27210747
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.04.018"; journalName: "Cell Rep"; date: 
            "2016-05-31- 31"; volume: "15"; issue: "9"; pages: "2025-37"
COMMENT     Sequence Label: pCDNA3.1(-) + SLNCR1 _1521-2092
FEATURES             Location/Qualifiers
     source          1..7217
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2811..2828)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2817..3041
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3087..3515
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3174..3193)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3384..3405
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3524..3544)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3529..3858
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3709..3844
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3771..3790
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3925..4719
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3979..3998)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4589..4608
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4893..5014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4930..4949)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4984..5003
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5063..5079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5063..5079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5076..5098)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5087..5103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5111..5117,5118..5135,5136..5141))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5156..5177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5294..5311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5465..6050)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5545..5564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6221..7012,7013..7081))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6844..6863
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7082..7186)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
      961 accacactgg actagtggat ccgagctcgg taccaagctt cccgggatac ttaggatcca
     1021 gggcgcgcag gcggcgcggg tgcgcggtgc ggcgctggta tccagaggac gcggtcaccg
     1081 cctctggcat ttgtcgttct gcgcttctcc gcaaggaccc tctgttaggc aggcgcccac
     1141 cgtaagcctc ccgggccttg tgaacctgca aacccaagtc tgagagacga tccgccttca
     1201 gcgctttcca gcttggcaga gaggctttcc cggcggggat ctttggttgg cgctggcgat
     1261 gcgcggggaa gaaaggcgag gagcggcgtc caggctgggt gatgtcccag cacgagtagg
     1321 cgggatgcgc tcgcttggtc ctccgggcgc ccggtccctg cccgcgtcgc gcgcccaccc
     1381 ctggggacga gaaggcggcc gcctgaggac ccccgcccgc gacctccgcg agtctggagc
     1441 gcagaggaca gggtctggct gctctttggc cttggatgga aagtggggaa ttgggtgggg
     1501 ggctgcggac cccttaacgt ggattacttg gtgtgtatca gctgggctca gaagacccac
     1561 gacctcttct ccatccgtgg attgatttgt tctgcttaac agctgggtcg ccaagctgga
     1621 ggtatttttc cctctccacc ctggtcttct cctgtaacgt gtggccgcct tttccagcac
     1681 ggcctcctgc cttcctggtg cactttttgg agaacgtggt ggaatcagag gtttctggct
     1741 gactcggtgg gtgctttgaa ccaggaaagg acaagaaaga ggatgggaag gactgatcca
     1801 cattcccacc aggaagttta gcagaacccc cgcgtgccac ctggacccct tggaaggacc
     1861 tggctcaggc tggaccacct cttgagaggc aggagctctg gatttgatca agaattcttt
     1921 gctgagcatg gtgcctcatg cctataatcc caacactttg ggaggccagt gtgggaggat
     1981 ctcttgagcc caggagttca agactagcct gggcaacaca gagagacccc atctctaaaa
     2041 taataataat aataaaataa aaaattagca gggcatggtg gcatgtgcct gtagtcccag
     2101 ctacccagga ggctgaggca agaggatggc tggagcctgg gatgttgagg ctgcaatgaa
     2161 ctgtgattac cccactgcac tccagcctgg gcaaaagagc gagagaccct gtctcaaata
     2221 ataataataa taataatctt attttggaga ataaagagac ctctggattt gaggtgccat
     2281 ttgggtagaa agaaaagacg tttacaccga gaaatagtct gtgttgccct gaaggagcag
     2341 agggatgcat cgctggaggt gacctacagt tgaagaagac tcattatgac agaccttgtc
     2401 cttcttcctt gtggaaagtg tttcctctgc tgctactgct catgagactc ttccccctcc
     2461 ctgtcccagg gaaccaaagg gctttctacc acaccctttc ttgccccccg cctcccatgt
     2521 ctgctgtgcc tttgtactca gcatgctgat gacacataca cacctgacaa tagcttgagt
     2581 cttctctgtt ccttttactc tgtagccaac atacacatga tttaaaaccc tttctaaata
     2641 tctatcatgg ttcatccttg tccaaatgca gagtcagagc tatttgtact tcattattat
     2701 ttccaaggcg aatagttggc tttctttttg caaaaataat taaagttttt gtatgttgca
     2761 gttgctctag ataagtatca cgtgaagctt aagtttaaac cgctgatcag cctcgactgt
     2821 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     2881 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     2941 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3001 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3061 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     3121 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3181 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3241 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3301 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3361 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3421 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3481 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     3541 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3601 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3661 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3721 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3781 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3841 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     3901 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     3961 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4021 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4081 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     4141 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4201 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4261 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4321 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4381 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4441 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4501 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4561 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4621 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4681 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     4741 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     4801 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     4861 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     4921 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4981 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     5041 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5101 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5161 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5221 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5281 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5341 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5401 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5461 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5521 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5581 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5641 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5701 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5761 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5821 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5881 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5941 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6001 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6061 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6121 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6181 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6241 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6301 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6361 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6421 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6481 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6541 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6601 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6661 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6721 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6781 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6841 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6901 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6961 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7021 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7081 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7141 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7201 aagtgccacc tgacgtc
//
