LOCUS       sequence-169377-       10916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human UTX with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Agger K, Cloos PA, Christensen J, Pasini D, Rose S, Rappsilber J, 
            Issaeva I, Canaani E, Salcini AE, Helin K
  TITLE     UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene 
            regulation and development.
  JOURNAL   Nature. 2007 Oct 11. 449(7163):731-4.
  PUBMED    17713478
REFERENCE   2  (bases 1 to 10916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Oct 11. 449(7163):731-4."
COMMENT     Sequence Label: pCMV-HA-UTX
FEATURES             Location/Qualifiers
     source          1..10916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1974..1992
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2030..2052)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2152..2171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2365..2387
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2379..2396
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2380..2396
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2807..2952
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3009..3803
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3063..3082)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3673..3692
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3997..4016)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4041..4088
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4381..4760
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4761..4964
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4915..4935
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4961..4985
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5076..5648
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             5728..5754
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5767..5791
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10095..10119)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10218..10237)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10283..10338
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10337..10356)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10696..10712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10696..10712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10709..10731)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10720..10736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10744..10750,10751..10768,10769..10774))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10789..10810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     2041 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     2101 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2161 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     2221 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     2281 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     2341 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
     2401 agcttcacgc tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta
     2461 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     2521 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     2581 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     2641 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     2701 ctgatggcgc aggggatcaa gatcctgctt catccccgtg gcccgttgct cgcgtttgct
     2761 ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga cacaaacccc
     2821 gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc
     2881 caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag
     2941 cgacccgctt aacagcgtca acagcgtgcc gcagatctga tcaagagaca ggatgaggat
     3001 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     3061 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     3121 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     3181 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     3241 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     3301 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     3361 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     3421 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     3481 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     3541 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     3601 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     3661 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     3721 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     3781 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     3841 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     3901 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     3961 gttcttcgcc caccccggga gatgggggag gctaactgaa acacggaagg agacaatacc
     4021 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     4081 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     4141 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     4201 gggtgaaggc ccagggctcg cagccaacgt cggggcggca agccctgcca tagccacggg
     4261 ccccgtgggt tagggacggc ggatcgcggc cctctagaga gcttggccca ttgcatacgt
     4321 tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt
     4381 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4441 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     4501 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     4561 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     4621 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     4681 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     4741 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     4801 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     4861 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     4921 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     4981 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     5041 ccagcctccg gtcgaccgat cctgagaact tcagggtgag tttggggacc cttgattgtt
     5101 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
     5161 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
     5221 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
     5281 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
     5341 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
     5401 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
     5461 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc
     5521 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     5581 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt
     5641 tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat
     5701 tcctcgacgg atcaattcac catggcatac ccatacgatg ttccagatta cgctagcgga
     5761 tcatcaacaa gtttgtacaa aaaagcaggc tctttaaagg aaccaattca gtcgactgga
     5821 tccggtacct ccatgaaatc ctgcggagtg tcgctcgcta ccgccgccgc tgccgccgcc
     5881 gctttcggtg atgaggaaaa gaaaatggcg gcgggaaaag cgagcggcga gagcgaggag
     5941 gcgtccccca gcctgacagc cgaggagagg gaggcgctcg gcggactgga cagccgcctc
     6001 tttgggttcg tgagatttca tgaagatggc gccaggacga aggccctact gggcaaggct
     6061 gttcgctgct atgaatctct aatcttaaaa gctgaaggaa aagtggagtc tgatttcttt
     6121 tgtcaattag gtcacttcaa cctcttattg gaagattatc caaaagcatt atctgcatac
     6181 cagaggtact acagtttaca gtctgactac tggaagaatg ctgccttttt atatggtctt
     6241 ggtttggtct acttccatta taatgcattt cagtgggcaa ttaaagcatt tcaggaggtg
     6301 ctttatgttg atcccagctt ttgtcgagcc aaggaaattc atttacgact tgggcttatg
     6361 ttcaaagtga acacagacta tgagtctagt ttaaagcatt ttcagttagc tttggttgac
     6421 tgtaatccct gcactttgtc caatgctgaa attcaatttc acattgccca cttatatgaa
     6481 acccagagga aatatcattc tgcaaaagaa gcttatgaac aacttttgca gacagagaat
     6541 ctttctgcac aagtaaaagc aactgtctta caacagttag gttggatgca tcacactgta
     6601 gatctcctgg gagataaagc caccaaggaa agctatgcta ttcagtatct ccaaaagtcc
     6661 ttggaggcag atcctaattc tggccagtcc tggtatttcc tcggaaggtg ctattcaagt
     6721 attgggaaag ttcaggatgc ctttatatct tacaggcagt ctattgataa atcagaagca
     6781 agtgcagata catggtgttc aataggtgtg ctatatcagc agcaaaatca gcccatggat
     6841 gctttacagg cctatatttg tgctgtacaa ttggaccatg gccatgctgc agcctggatg
     6901 gacctaggca ctctctacga atcctgcaac cagcctcagg atgccattaa atgctactta
     6961 aatgcaacta gaagcaaaag ttgtagtaat acctctgcac ttgcagcacg aattaagtat
     7021 ttacaggcat gtaaacctca tcatccaaat actgaacctg tattaggcct cagtcaaaca
     7081 ccaatttcac agcaatcctt gccactacac atgattcctt ctagccaagt agatgacctg
     7141 tccagtcctg ccaagaggaa aagaacatct agtccgacaa agaatacttc tgacaattgg
     7201 agtggtggac atgctgtgtc acatcctcca gtacagcaac aagctcattc atggtgtttg
     7261 acaccacaga aattacagca tttggaacag ctccgcgcaa atagaaataa tttaaatcca
     7321 gcacagaaac tgatgctgga acagctggaa agtcagtttg tcttaatgca acaacaccaa
     7381 atgagaccaa caggagttgc acaggtacga tctactggaa ttcctaatgg gccaacagct
     7441 gactcatcac tgcctacaaa ctcagtctct ggccagcagc cacagcttgc tctgaccaga
     7501 gtgcctagcg tctctcagcc tggagtccgt cctgcctgcc ctgggcagcc tttggccaat
     7561 ggaccctttt ctgcaggcca tgttccctgt agcacatcaa gaacgctggg aagtacagac
     7621 actattttga taggcaataa tcatataaca ggaagtggaa gtaatggaaa cgtgccttac
     7681 ctgcagcgaa acgcactcac tctacctcat aaccgcacaa acctgaccag cagcgcagag
     7741 gagccgtgga aaaaccaact atctaactcc actcaggggc ttcacaaagg tcagagttca
     7801 cattcggcag gtcctaatgg tgaacgacct ctctcttcca ctgggccttc ccagcatctc
     7861 caggcagctg gctctggtat tcagaatcag aacggacatc ccaccctgcc tagcaattca
     7921 gtaacacagg gggctgctct caatcacctc tcctctcaca ctgctacctc aggtggacaa
     7981 caaggcatta ccttaaccaa agagagcaag ccttcaggaa acatattgac ggtgcctgaa
     8041 acaagcaggc acactggaga gacacctaac agcactgcca gtgtcgaggg acttcctaat
     8101 catgtccatc agatgacggc agatgctgtt tgcagtccta gccatggaga ttctaagtca
     8161 ccaggtttac taagttcaga caatcctcag ctctctgcct tgttgatggg aaaagccaat
     8221 aacaatgtgg gtactggaac ctgtgacaaa gtcaataaca tccacccagc tgttcataca
     8281 aagactgata actctgttgc ctcttcacca tcttcagcca tttcaacagc aacaccttct
     8341 ccaaaatcca ctgagcagac aaccacaaac agtgttacca gccttaacag ccctcacagt
     8401 gggctacaca caattaatgg agaagggatg gaagaatctc agagccccat gaaaacagat
     8461 ctgcttctgg ttaaccacaa acctagtcca cagatcatac catcaatgtc tgtgtccata
     8521 taccccagct cagcagaagt tctgaaggca tgcaggaatc taggtaaaaa tggcttatct
     8581 aacagtagca ttttgttgga taaatgtcca ccaccaagac caccatcttc accataccct
     8641 cccttgccaa aggacaagtt gaatccacct acacctagta tttacttgga aaataaacgt
     8701 gatgctttct ttcctccatt acatcaattt tgtacaaatc cgaacaaccc tgttacagta
     8761 atacgtggcc ttgctggagc tcttaagtta gacctgggac ttttctctac taaaactttg
     8821 gtggaagcta acaatgaaca tatggtagaa gtgaggacac agttgttgca gccagcagat
     8881 gaaaactggg atcccactgg aacaaagaaa atctggcatt gtgaaagtaa tagatctcat
     8941 actacaattg ctaaatatgc acagtaccag gcctcctcat tccaggaatc attgagagaa
     9001 gaaaatgaaa aaagaagtca tcataaagac cactcagata gtgaatctac atcgtcagat
     9061 aattctggga ggaggaggaa aggacccttt aaaaccataa agtttgggac caatattgac
     9121 ctatctgatg acaaaaagtg gaagttgcag ctacatgagc tgactaaact tcctgctttt
     9181 gtgcgtgtcg tatcagcagg aaatcttcta agccatgttg gtcataccat attgggcatg
     9241 aacacagttc aactatacat gaaagttcca gggagcagaa caccaggtca tcaggaaaat
     9301 aacaacttct gttcagttaa cataaatatt ggcccaggtg actgtgaatg gtttgttgtt
     9361 cctgaaggtt actggggtgt tctgaatgac ttctgtgaaa aaaataattt gaatttccta
     9421 atgggttctt ggtggcccaa tcttgaagat ctttatgaag caaatgttcc agtgtatagg
     9481 tttattcagc gacctggaga tttggtctgg ataaatgcag gcactgttca ttgggttcag
     9541 gctattggct ggtgcaacaa cattgcttgg aatgttggtc cacttacagc ctgccagtat
     9601 aaattggcag tggaacggta cgaatggaac aaattgcaaa gtgtgaagtc aatagtaccc
     9661 atggttcatc tttcctggaa tatggcacga aatatcaagg tctcagatcc aaagcttttt
     9721 gaaatgatta agtattgtct tctaagaact ctgaagcaat gtcagacatt gagggaagct
     9781 ctcattgctg caggaaaaga gattatatgg catgggcgga caaaagaaga accagctcat
     9841 tactgtagca tttgtgaagt ggaggttttt gatctgcttt ttgtcactaa tgagagtaat
     9901 tcacgaaaga cctacatagt acattgccaa gattgtgcac gaaaaacaag cggaaacttg
     9961 gaaaactttg tggtgctaga acagtacaaa atggaggacc tgatgcaagt ctatgaccaa
    10021 tttacattag ctcctccatt accatccgcc tcatcttgaa agggcgaatt cgcggccgca
    10081 ctcgagatat ctagacccag ctttcttgta caaagtggtt gatgatccgt cgaggaattc
    10141 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
    10201 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
    10261 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
    10321 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
    10381 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
    10441 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
    10501 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
    10561 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
    10621 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cggatcggcc
    10681 gcaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgct
//
