LOCUS       sequence-169387-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cancer-associated ASXL1 truncation mutant in mammalian expression 
            vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Balasubramani A, Larjo A, Bassein JA, Chang X, Hastie RB, Togher SM,
            Lahdesmaki H, Rao A
  TITLE     Cancer-associated ASXL1 mutations may act as gain-of-function 
            mutations of the ASXL1-BAP1 complex.
  JOURNAL   Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307.
  PUBMED    26095772
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-06-22"; volume: "6:7307. doi"; pages: " 
            10.1038/ncomms8307"
COMMENT     Sequence Label: pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     181..197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(257..322)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 acttgcccct tgctccatac cacccccctc caccccataa tattatagaa ggacacctag
       61 tcagacaaaa tgatgcaact taattttatt aggacaaggc tggtgggcac tggagtggca
      121 acttccaggg ccaggagagg cactggggag gggtcacagg gatgccaccc gggatctgtt
      181 caggaaacag ctatgaccgc ggccgcaatc tagagtcgac aagcttgata tcggtaccag
      241 atctgaattc ttatcattta tcatcatcat ctttataatc gatgtcgtgg tccttgtagt
      301 cgccgtcgtg gtccttgtag tcgtaagtgg gctgaccttt aaccacccag ggtggtttga
      361 tacgtgaaag ttgaatccgg atgggcggga ctttgggctc ctctttagtg ggctgtggct
      421 tttcggtgtg aacactttca attgtgttac gaaaggactg acgatcttca agccggggct
      481 tcttttcggg aaaggatgca gaggccgcct gctcaaagga tttcctcttc tgatccttgg
      541 gttcctgatc cacagtttcc tgaggcaggc taggaatcct gtctggagat gcagaggcac
      601 gtgccaagtt gtctggctca gcctggatcc gagaagccac agactcaact gggaactcgg
      661 gaccctccag gtcgggtgct gcagaggatg tgcctggcag atggggactg ctcagccctg
      721 ctgggtcagt cttagcctcc ccatccttgt agaggggaac atctgaggcc acagattttg
      781 catccttagc aacccctgct tgttctgact cctgtttttt gtacagattc cttctggctc
      841 tggttcggag atctggccga gagcgtttct taaaatgccc atctcgctgt cgggtggctg
      901 gaccacgctg tatacgcact gattctcctg ggacacacaa gccacttttg atttcagcct
      961 cctcctgg
//
