LOCUS       sequence-169388-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cancer-associated ASXL1 truncation mutant in mammalian expression 
            vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Balasubramani A, Larjo A, Bassein JA, Chang X, Hastie RB, Togher SM,
            Lahdesmaki H, Rao A
  TITLE     Cancer-associated ASXL1 mutations may act as gain-of-function 
            mutations of the ASXL1-BAP1 complex.
  JOURNAL   Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307.
  PUBMED    26095772
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-06-22"; volume: "6:7307. doi"; pages: " 
            10.1038/ncomms8307"
COMMENT     Sequence Label: pCMV6-XL4 ASXL1 (1-479) 3x FLAG
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagaat tttgtaatac gactcactat agggcggccg
       61 cgaattcatg aaggacaaac agaagaagaa gaaggagcgc acgtgggccg aggccgcgcg
      121 cctggtatta gaaaactact cggatgctcc aatgacacca aaacagattc tgcaggtcat
      181 agaggcagaa ggactaaagg aaatgagaag tgggacttcc cctctcgcat gcctcaatgc
      241 tatgctacat tccaattcaa gaggaggaga ggggttgttt tataaactgc ctggccgaat
      301 cagccttttc acgctcaaga aggatgccct gcagtggtct cgccatccag ctacagtgga
      361 gggagaggag ccagaggaca cggctgatgt ggagagctgt gggtctaatg aagccagcac
      421 tgtgagtggt gaaaacgatg tatctcttga tgaaacatct tcgaacgcat cctgttctac
      481 agaatctcag agtcgacctc tttccaatcc cagggacagc tacagagctt cctcacaggc
      541 gaacaaacaa aagaaaaaga ctggggtgat gctgcctcga gttgtcctga ctcctctgaa
      601 ggtaaacggg gcccacgtgg aatctgcatc agggttctcg ggctgccacg ccgatggcga
      661 gagcggcagc ccgtccagca gcagcagcgg ctctctggcc ctgggcagcg ctgctattcg
      721 tggccaggcc gaggtcaccc aggaccctgc cccgctcctg agaggcttcc ggaagccagc
      781 cacaggtcaa atgaagcgca acagagggga agaaatagat tttgagacac ctgggtccat
      841 tcttgtcaac accaacctcc gtgccctgat caactctcgg accttccatg ccttaccatc
      901 acacttccag cagcagctcc tcttcctcct gcctgaagta gacagacagg tggggacgga
      961 tggcctgttg cgtctcagca gcagtgcact aaata
//
