LOCUS       sequence-169389-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cancer-associated ASXL1 truncation mutant in mammalian expression 
            vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Balasubramani A, Larjo A, Bassein JA, Chang X, Hastie RB, Togher SM,
            Lahdesmaki H, Rao A
  TITLE     Cancer-associated ASXL1 mutations may act as gain-of-function 
            mutations of the ASXL1-BAP1 complex.
  JOURNAL   Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307.
  PUBMED    26095772
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-06-22"; volume: "6:7307. doi"; pages: " 
            10.1038/ncomms8307"
COMMENT     Sequence Label: pCMV6-XL4 ASXL1 (1-479) 3x FLAG
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     181..197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(263..328)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 acttgcccct tgctccatac cacccccctc caccccataa tattatagaa ggacacctag
       61 tcagacaaaa tgatgcaact taattttatt aggacaaggc tggtgggcac tggagtggca
      121 acttccaggg ccaggagagg cactggggag gggtcacagg gatgccaccc gggatctgtt
      181 caggaaacag ctatgaccgc ggccgcaatc tagagtcgac aagcttgata tcggtaccag
      241 atctgaattc ttatttataa tctttatcat catcatcttt ataatcgatg tcgtggtcct
      301 tgtagtcgcc gtcgtggtcc ttgtagtcgg gaccctccag gtcgggtgct gcagaggatg
      361 tgcctggcag atggggactg ctcagccctg ctgggtcagt cttagcctcc ccatccttgt
      421 agaggggaac atctgaggcc acagattttg catccttagc aacccctgct tgttctgact
      481 cctgtttttt gtacagattc cttctggctc tggttcggag atctggccga gagcgtttct
      541 taaaatgccc atctcgctgt cgggtggctg gaccacgctg tatacgcact gattctcctg
      601 ggacacacaa gccacttttg atttcagcct cctcctggcc cacgttctgc tgcaatgact
      661 cttctttggt caaacccagc ttctgtccat agtagtcttc aaagaacttt tctttccatt
      721 gttccacctt cttttccttc tccatttcct gtcgtatcct gacttgcatc tcatgagtaa
      781 attcaccatc agccaggcgc tcccgccagc tctgagccgc atgggtaaaa aactcgttat
      841 ttagtgcact gctgctgaga cgcaacaggc catccgtccc cacctgtctg tctacttcag
      901 gcaggaggaa gaggagctgc tgctggaagt gtgatggtaa ggcatggaag gtccgagagt
      961 tgatcagggc acggaggttg gtgttgac
//
