LOCUS       sequence-169421-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of fusion protein with cell penetrating 
            peptide, antennapedia fragment, at C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Chien WM, Liu Y, Chin MT
  TITLE     Genomic DNA recombination with cell-penetrating peptide-tagged cre 
            protein in mouse skeletal and cardiac muscle.
  JOURNAL   Genesis. 2014 Jul;52(7):695-701. doi: 10.1002/dvg.22782. Epub 2014 
            May 5.
  PUBMED    24753043
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22782";
            journalName: "Genesis"; date: "2014-07"; volume: "52"; issue: "7"; 
            pages: "695-701"
COMMENT     Sequence Label: pET16b-Antp
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..66
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             79..90
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(222..240)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      236..283
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(458..463,464..480,481..486))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(513..531)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        599..703
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(922..941)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggccatc atcatcatca tcatcatcat
       61 catcacagca gcggccatat cgaaggtcgt catatgctcg aggagtccgg cggaggcgga
      121 tctcgccaga taaagatttg gttccagaat cggcgcatga agtggaagaa gggatccggc
      181 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      241 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      301 atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
      361 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
      421 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc
      481 tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      541 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      721 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      781 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      841 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      901 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
      961 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt a
//
