LOCUS       sequence-169422-        1147 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent probe for cGMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Matsuda S, Harada K, Ito M, Takizawa M, Wongso D, Tsuboi T, 
            Kitaguchi T
  TITLE     Generation of a cGMP Indicator with an Expanded Dynamic Range by 
            Optimization of Amino Acid Linkers between a Fluorescent Protein and
            PDE5alpha.
  JOURNAL   ACS Sens. 2017 Jan 27;2(1):46-51. doi: 10.1021/acssensors.6b00582. 
            Epub 2016 Dec 29.
  PUBMED    28722423
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.6b00582"; journalName: "ACS Sens"; date: 
            "2017-01-27- 27"; volume: "2"; issue: "1"; pages: "46-51"
COMMENT     Sequence Label: Green cGull
FEATURES             Location/Qualifiers
     source          1..1147
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(52..73)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(313..332)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             883..1134
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1071..1092
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcgagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
       61 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      181 cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtactc tatgcgcgga gtgcatggac tcatctctgc ggatcgctat
      481 tccctgttcc ttgtctgtga ggacagccct aaagacaaat tcctcatcag ccgcctcttt
      541 gatgttgctg aaggttcaac attggaggaa gcctcaaata actgtatccg tttagagtgg
      601 aacaaaggca ttgtgggaca tgtggcagct tttggcgagc ccttgaacat caaagatgcc
      661 tatgaggacc cccggttcaa tgcagaagtt gaccaaatta caggctacaa gacacaaagt
      721 atcctttgta tgccaattaa gaaccacagg gaagaggttg ttggtgtagc tcaggccatc
      781 aacaagaaat caggaaatgg tgggaccttc actgaaaaag atgaaaagga ctttgcggcc
      841 tacttggtga atgtttttac gcacaacgtc tatatcatgg ccgacaagca gaagaacggc
      901 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      961 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1021 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1081 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaattc
     1141 gaagctt
//
