LOCUS       sequence-169432-        7290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Chen B, Cepko CL
  TITLE     HDAC4 regulates neuronal survival in normal and diseased retinas.
  JOURNAL   Science. 2009 Jan 9. 323(5911):256-9.
  PUBMED    19131628
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Jan 9. 323(5911):256-9."
COMMENT     Sequence Label: pCAG-HIF1 alpha
FEATURES             Location/Qualifiers
     source          1..7290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..641
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     intron          642..1658
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1582..1604
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1666..1685
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1739..1748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4311..4330)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4376..4431
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4430..4449)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4792..4808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4792..4808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4805..4827)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4816..4832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4840..4846,4847..4864,4865..4870))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4885..4906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4965..5160
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5011..5146
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5166..5300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5203..5222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5257..5276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5368..5385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5539..6127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5619..5638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6298..7089,7090..7158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6921..6940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7159..7263)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcggg gcgaggggcg gggcggggcg aggcggagag
      541 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      601 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
      661 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
      721 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
      781 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
      841 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
      901 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
      961 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     1021 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     1081 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     1141 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     1201 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     1261 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     1321 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     1381 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     1441 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     1501 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     1561 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     1621 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     1681 ttgtgctgtc tcatcatttt ggcaaagaat tcctcgagga tatcgcggcc gctgagccgc
     1741 caccatggag ggcgccggcg gcgcgaacga caagaaaaag ataagttctg aacgtcgaaa
     1801 agaaaagtct cgagatgcag ccagatctcg gcgaagtaaa gaatctgaag ttttttatga
     1861 gcttgctcat cagttgccac ttccacataa tgtgagttcg catcttgata aggcctctgt
     1921 gatgaggctt accatcagct atttgcgtgt gaggaaactt ctggatgctg gtgatttgga
     1981 tattgaagat gacatgaaag cacagatgaa ttgcttttat ttgaaagcct tggatggttt
     2041 tgttatggtt ctaacagatg atggtgacat gatttacatt tctgataatg tgaacaaata
     2101 catgggatta actcagtttg aactaactgg acacagtgtg tttgatttta ctcatccatg
     2161 tgaccatgag gaaatgagag aaatgcttac acacagaaat ggccttgtga aaaagggtaa
     2221 agaacaaaac acacagcgaa gcttttttct cagaatgaag tgtaccctaa ctagccgagg
     2281 aagaactatg aacataaagt ctgcaacatg gaaggtattg cactgcacag gccacattca
     2341 cgtatatgat accaacagta accaacctca gtgtgggtat aagaaaccac ctatgaccta
     2401 cttggtgctg atttgtgaac ccattcctca cccatcaaat attgaaattc ctttagatag
     2461 caagactttc ctcagtcgac acagcctgga tatgaaattt tcttattgtg atgaaagaat
     2521 taccgaattg atgggatatg agccagaaga acttttaggc cgctcaattt atgaatatta
     2581 tcatgatttg gactctgatc atctgaccaa aactcatcat gatatgttta ctaaaggaca
     2641 agtcaccaca ggacagtaca ggatgcttgc caaaagaggt ggatatgtct gggttgaaac
     2701 tcaagcaact gtcatatata acaccaagaa ttctcaacca cagtgcattg tatgtgtgaa
     2761 ttacgttgtg agtggtatta ttcagcacga cttgattttc tcccttcaac aaacagaatg
     2821 tgtccttaaa ccggttgaat cttcagatat gaaaatgact cagctattca ccaaagttga
     2881 atcagaagat acaagtagcc tctttgacaa acttaagaag gaacctgatg ctttaacttt
     2941 gctggcccca gccgctggag acacaatcat atctttagat tttggcagca acgacacaga
     3001 aactgatgac cagcaacttg aggaagtacc attatataat gatgtaatgc tcccctcacc
     3061 caacgaaaaa ttacagaata taaatttggc aatgtctcca ttacccaccg ctgaaacgcc
     3121 aaagccactt agaagtagtg ctgaccctgc actcaatcaa gaagttgcat taaaattaga
     3181 accaaatcca gagtcactgg aactttcttt taccatgccc cagattcagg atcagacacc
     3241 tagtccttcc gatggaagca ctagacaaag ttcacctgag cctaatagtc ccagtgaata
     3301 ttgtttttat gtggatagtg atatggtcaa tgaattcaag ttggaattgg tagaaaaact
     3361 ttttgctgaa gacacagaag caaagaaccc attttctact caggacacag atttagactt
     3421 ggagatgtta gctccctata tcccaatgga tgatgacttc cagttacgtt ccttcgatca
     3481 gttgtcacca ttagaaagca gttccgcaag ccctgaaagc gcaagtcctc aaagcacagt
     3541 tacagtattc cagcagactc aaatacaaga acctactgct aatgccacca ctaccactgc
     3601 caccactgat gaattaaaaa cagtgacaaa agaccgtatg gaagacatta aaatattgat
     3661 tgcatctcca tctcctaccc acatacataa agaaactact agtgccacat catcaccata
     3721 tagagatact caaagtcgga cagcctcacc aaacagagca ggaaaaggag tcatagaaca
     3781 gacagaaaaa tctcatccaa gaagccctaa cgtgttatct gtcgctttga gtcaaagaac
     3841 tacagttcct gaggaagaac taaatccaaa gatactagct ttgcagaatg ctcagagaaa
     3901 gcgaaaaatg gaacatgatg gttcactttt tcaagcagta ggaattggaa cattattaca
     3961 gcagccagac gatcatgcag ctactacatc actttcttgg aaacgtgtaa aaggatgcaa
     4021 atctagtgaa cagaatggaa tggagcaaaa gacaattatt ttaataccct ctgatttagc
     4081 atgtagactg ctggggcaat caatggatga aagtggatta ccacagctga ccagttatga
     4141 ttgtgaagtt aatgctccta tacaaggcag cagaaaccta ctgcagggtg aagaattact
     4201 cagagctttg gatcaagtta actgagcggc cgcactcctc aggtgcaggc tgcctatcag
     4261 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     4321 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     4381 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     4441 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     4501 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     4561 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     4621 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     4681 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     4741 cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct
     4801 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     4861 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4921 actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca
     4981 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5041 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5101 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5161 aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5221 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5281 tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5341 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5401 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5461 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5521 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5581 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5641 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5701 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5761 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5821 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5881 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5941 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6001 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6061 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6181 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6241 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6301 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6361 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6421 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6481 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6541 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6601 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6661 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6721 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6781 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6841 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6901 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6961 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7021 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7081 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7141 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7201 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7261 gcgcacattt ccccgaaaag tgccacctgg
//
