LOCUS       sequence-169440-       10777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector expressing TIAM1-IRES-RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander
            ES, Sabatini DM
  TITLE     Gene Essentiality Profiling Reveals Gene Networks and Synthetic 
            Lethal Interactions with Oncogenic Ras.
  JOURNAL   Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 
            10.1016/j.cell.2017.01.013.
  PUBMED    28162770
REFERENCE   2  (bases 1 to 10777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013."
COMMENT     Sequence Label: pMXs3-TIAM1
FEATURES             Location/Qualifiers
     source          1..10777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1923..1932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6738..6803
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(6831..7405,7406..7408,7409..7417)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6998..7015)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7225..7244
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7418..8113
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     LTR             8282..8751
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8881..8898)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9052..9640)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9132..9151)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9804..10595,10596..10664))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10427..10446
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10665..10769)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccgccaccat gggaaacgca gaaagtcaac atgtagagca cgagttttat ggagaaaagc
     1981 atgccagcct ggggcgcaag cacacttccc gctccctgcg cctctcgcac aagacgcgga
     2041 ggaccaggca cgcttcctcg gggaaggtga tccacaggaa ctccgaagtg agcacccgat
     2101 ccagcagcac ccccagcatc ccccagtccc tggctgaaaa tggcctggag cccttctccc
     2161 aagatggtac cctagaagac ttcgggagcc ccatctgggt ggaccgagtg gacatgggct
     2221 tgagacctgt gtcttacact gactcttctg tcactcccag cgtagacagc agcatcgtcc
     2281 tcacagcagc ctctgtgcag agcatgccag acactgagga gagcaggctt tacggggatg
     2341 acgctacata tttggctgag ggaggcagga ggcagcattc ctatacatcc aatgggccca
     2401 ctttcatgga gacggcgagc tttaagaaga aacgctccaa atctgcagac atctggcggg
     2461 aggacagcct ggaattctca ctctctgatc tgagccaaga acatttaaca agcaacgaag
     2521 aaatcttggg ttccgccgaa gagaaggact gcgaggaggc tcgggggatg gaaacgcggg
     2581 cgagtccgcg gcagctcagc acctgtcaga gagccaattc cttgggtgac ttgtatgctc
     2641 agaaaaactc tggagtgaca gcaaacgggg ggccggggag caaatttgca ggctactgtc
     2701 ggaatttggt gtctgatatt cccaatcttg caaaccataa gatgccacca gctgctgctg
     2761 aagagactcc tccgtacagt aattataaca cacttccctg taggaaatct cactgtctct
     2821 ctgaaggtgc caccaaccca caaattagcc atagcaacag catgcaaggc agaagagcta
     2881 aaacaactca ggatgttaat gcaggcgagg gcagtgagtt tgcagacagt gggattgaag
     2941 gggccactac cgacacggac ctcctgtcca ggcgatctaa tgccaccaac tccagctact
     3001 cacccaccac aggccgggcc tttgtgggca gcgacagcgg cagcagctcc accggggatg
     3061 cggctcgtca gggggtgtac gagaacttcc ggcgggagct ggagatgagc accaccaaca
     3121 gcgagagcct ggaggaggcc ggctcggcgc acagcgatga gcagagcagc ggcaccctga
     3181 gctctccggg ccagtcggac atcctgctga ccgccgcaca gggcacggtg cgcaaggccg
     3241 gcgccctggc cgtcaagaac ttcctggtgc acaagaagaa caagaaggtg gagtcagcca
     3301 cccggaggaa gtggaagcac tactgggtgt ccctgaaagg atgcacgcta tttttctacg
     3361 agagcgacgg caggtctggg atagaccaca acagcatccc caaacacgcc gtctgggtgg
     3421 agaacagcat tgtgcaggcg gtgcctgagc accccaagaa ggactttgtc ttctgcctca
     3481 gcaattccct gggtgatgcc ttcctttttc agaccactag ccagacggag cttgaaaact
     3541 ggatcaccgc catccactct gcctgcgcca ctgcggtcgc gaggcaccac cacaaggaag
     3601 acacgctccg actcctgaaa tcagagatca aaaaactgga acagaagatt gacatggatg
     3661 aaaagatgaa gaaaatgggt gaaatgcagc tgtcttcagt cactgactca aagaaaaaga
     3721 aaacaatatt agatcagatc tttgtctggg agcaaaatct cgagcagttc caaatggacc
     3781 tgtttcgttt ccgctgttat ttagccagcc ttcagggtgg ggagctgcca aaccccaaaa
     3841 ggcttctcgc ttttgcaagt cgaccaacga aagtggccat gggccgcctt ggaatctttt
     3901 cggtatcatc gtttcatgcc ctggtggcag cacgcactgg tgaaactgga gtgagaagac
     3961 gtactcaggc catgtccaga tccgcgagca agcgaaggag caggttttct tctctgtggg
     4021 gtctggatac tacctccaaa aagaagcagg gacggccaag catcaatcag gtgtttggag
     4081 agggaaccga agctgtaaag aaatctttag agggaatatt tgatgacatt gttccagatg
     4141 gcaagaggga gaaagaagtg gtcttaccta acgttcacca gcacaaccct gactgcgaca
     4201 tttgggtcca cgagtatttc actccatcct ggttctgtct gcccaataat cagcctgccc
     4261 tgacggtcgt ccggccaggc gacactgcac gggacaccct ggagctgatt tgcaagacac
     4321 atcaactgga tcattctgct cattacctgc gcctgaaatt tctaatagaa aacaaaatgc
     4381 agctctatgt tccacagccc gaggaagaca tctatgagct gctgtacaaa gaaattgaaa
     4441 tctgtccaaa agtcactcag agcatccaca ttgagaagtc agatacagct gctgatactt
     4501 acgggttttc actttcttct gtggaagaag atggtattcg aaggctgtac gtgaatagtg
     4561 tgaaggaaac cggtttagct tccaagaaag gcctgaaagc aggagatgag attcttgaga
     4621 tcaataatcg tgctgctgac gccctgaact cttctatgct caaagatttc ctctcacagc
     4681 cctcgctggg cctcctggtg aggacctacc ccgagctgga ggaaggagtg gagctgctgg
     4741 aaagcccgcc ccaccgagtg gacggccctg ccgaccttgg cgagagcccc ctcgcctttc
     4801 tcaccagcaa cccagggcac agcctttgca gcgagcaggg cagcagtgct gagaccgctc
     4861 cagaggagac cgaggggcca gacttggaat cctcagatga gactgatcac agcagcaaga
     4921 gtacagaaca ggtggccgca ttttgccgca gtttgcatga gatgaacccc tctgaccaga
     4981 gcccatctcc tcaggactcc acggggcctc agctggcgac catgagacaa ctctcggatg
     5041 cagataagct gcgcaaggtg atctgcgagc tcctggagac ggagcgcacc tacgtgaagg
     5101 atttaaactg tcttatggag agatacctaa agcctcttca aaaagaaact tttctcaccc
     5161 aggatgagct tgacgtgctt tttggaaatt taacggaaat ggtagagttt caagtagaat
     5221 tccttaaaac tctagaagat ggagtgagac tggtacctga tttggaaaag cttgagaagg
     5281 ttgatcaatt taagaaagtg ctgttctctc tggggggatc attcctgtat tatgctgacc
     5341 gcttcaagct ctacagtgcc ttctgcgcca gccacacaaa agttcccaag gtcctggtga
     5401 aagccaagac agacacggct ttcaaggcat tcttggatgc ccagaacccg aagcagcagc
     5461 actcatccac gctggagtcg tacctcatca agcccatcca gaggatcctc aagtacccac
     5521 ttctgctcag ggagctgttc gccctgaccg atgcggagag cgaggagcac taccacctgg
     5581 acgtggccat caagaccatg aacaaggttg ccagtcacat caatgagatg cagaaaatcc
     5641 atgaagagtt tggggctgtg tttgaccagc tgattgctga acagactggt gagaaaaaag
     5701 aggttgcaga tctgagcatg ggagacctgc ttttgcacac taccgtgatc tggctgaacc
     5761 cgccggcctc gctgggcaag tggaaaaagg aaccagagtt ggcagcattc gtcttcaaaa
     5821 ctgctgtggt ccttgtgtat aaagatggtt ccaaacagaa gaagaaactt gtaggatctc
     5881 acaggctttc catttatgag gactgggacc ccttcagatt tcgacacatg atccccacgg
     5941 aagcgctgca ggttcgagct ttggcgagtg cagatgcaga ggcaaatgcc gtgtgtgaaa
     6001 ttgtccatgt aaaatccgag tctgaaggga ggccggagag ggtctttcac ttgtgctgca
     6061 gctccccaga gagccgaaag gatttcctaa aggctgtgca ttcaatcctg cgtgataagc
     6121 acagaagaca gctcctcaaa accgagagcc ttccctcatc ccagcaatat gtcccttttg
     6181 gaggcaaaag attgtgtgca ctgaaggggg ccaggccggc catgagcagg gcagtgtctg
     6241 ccccaagcaa gtctcttggg aggaggaggc ggcggctggc tcgaaacagg tttaccattg
     6301 attctgatgc cgtctccgca agcagcccgg agaaagagtc ccagcagccc cccggtggcg
     6361 gggacactga ccgatgggta gaggagcagt ttgatcttgc tcagtatgag gagcaagatg
     6421 acatcaagga gacagacatc ctcagtgacg atgatgagtt ctgtgagtcc gtgaagggtg
     6481 cctcagtgga cagagacctg caggagcggc ttcaggccac ctccatcagt cagcgggaaa
     6541 gaggccggaa aaccctggat agtcacgcgt cccgcatggc acagctcaag aagcaagctg
     6601 ccctgtcggg gatcaatgga ggcctggaga gcgcaagcga ggaagtcatt tgggttaggc
     6661 gtgaagactt tgccccctcc aggaaactga acactgagat ctgagcggcc gcaggaggcg
     6721 gtggaggcgg tgcaggcgac tacaaagacc atgacggtga ttataaagat catgacattg
     6781 attacaagga tgacgatgac aagtaataat aaattccgcc cccccccccc cccctctccc
     6841 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     6901 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     6961 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     7021 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     7081 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     7141 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     7201 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     7261 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     7321 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     7381 ttcctttgaa aaacacgatg ataatatggc cacaaccatg agcgagctga tcaaggagaa
     7441 catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
     7501 cgagggcgaa ggcaagccct acgagggcac ccagaccatg aagatcaagg tggtcgaggg
     7561 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaagc
     7621 cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt
     7681 cacatgggag agaatcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     7741 cagcttccag aacggctgca tcatctacaa cgtcaagatc aacggggtga acttcccatc
     7801 caacggccct gtgatgcaga agaaaacacg cggctgggag gccaacaccg agatgctgta
     7861 ccccgctgac ggcggcctga gaggccacag ccagatggcc ctgaagctcg tgggcggggg
     7921 ctacctgcac tgctccttca agaccacata cagatccaag aaacccgcta agaacctcaa
     7981 gatgcccggc ttccacttcg tggaccacag actggaaaga atcaaggagg ccgacaaaga
     8041 gacctacgtc gagcagcacg agatggctgt ggccaagtac tgcgacctcc ctagcaaact
     8101 ggggcacaga taagtacgat aaaataaaag attttattta gtctccagaa aaagggggga
     8161 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     8221 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
     8281 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     8341 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     8401 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     8461 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     8521 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     8581 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     8641 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     8701 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca
     8761 tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca
     8821 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8881 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8941 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9001 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9061 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9121 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9181 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9241 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9301 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9361 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9421 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9481 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9541 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9601 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9661 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9721 atcaaaaagg atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata
     9781 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9841 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9901 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9961 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10021 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10081 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10141 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10201 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10261 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10321 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10381 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10441 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10501 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10561 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10621 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10681 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10741 tagaaaaata aacaaatagg ggttccgcgc acatttc
//
