LOCUS       sequence-169447-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of E. coli SRAP ortholog YedK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE
  TITLE     Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease.
  JOURNAL   Cell Rep. 2017 Oct 10;21(2):482-494. doi: 
            10.1016/j.celrep.2017.09.055.
  PUBMED    29020633
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.09"; journalName: "Cell Rep"; date: 
            "2017-10-10- 10"; volume: "21"; issue: "2"; pages: "482-494"
COMMENT     Sequence Label: pET30a-YedK
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             668..694
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             704..721
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agatctggga cgctttgccc aatcccaaac gcgtgaagat taccttgcgc ttctcgcgga
       61 agatattgaa cgcgatattc cctatgatcc cgaacccatt ggcagataca acgtcgcgcc
      121 gggaaccaaa gtcctactgc tcagtgaacg tgatgaacac cttcatctgg atccggtttt
      181 ctggggatat gctcccggat ggtgggataa accgccgctg attaacgccc gcgtagaaac
      241 tgcggcaacc agtcgtatgt ttaaaccgct ctggcaacat ggtcgggcaa tctgttttgc
      301 cgatggctgg tttgagtgga aaaaagaagg cgacaaaaaa cagccttttt ttatctatcg
      361 tgctgatgga caacctattt ttatggccgc gataggnagc acaccatttg agcgtggtga
      421 cgaagccgaa ggatttttga tcgtcactgc tgcggcagat caaggtctgg tagatattca
      481 tgaccgccgc ccactggtac tgtcgccaga agctgcgcgg gaatggatgc ggcaagagat
      541 tatcggtaaa gaagcctcag aaatcgcggc cagtggctgt gttccagcaa accagttctc
      601 ctgnnacccg gtatcncgcg cggtgggtaa tgntaaaaac cagggcgcgg agttaattcn
      661 acctgtttac ccntacgatg ttccagatta cgctggactc gagcaccacc accaccacca
      721 ctga
//
