LOCUS       sequence-169484-       10194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expressing YFP in mammalian cells upon Cre co-expression. Can be
            made into Lentivirus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y,
            So PT, Nedivi E
  TITLE     Inhibitory Synapses Are Repeatedly Assembled and Removed at 
            Persistent Sites In Vivo.
  JOURNAL   Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010.
  PUBMED    26853302
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010."
COMMENT     Sequence Label: FU - dio YFP - W
FEATURES             Location/Qualifiers
     source          1..10194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    270..391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(281..300)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     335..354
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(521..895)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(914..961)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1009..1338)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1023..1158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1077..1096)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1323..1343
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1352..1780)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1462..1483)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1674..1693
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1826..2050
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2039..2056
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2082..2262
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     2767..2783
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2768..2784
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2787..3375
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2893..2904
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3302..3322
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(3400..3433)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3484..3517)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      3524..3533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3530..3532,3533..3535,3536..4249)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3575..3596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3836..3855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4183..4204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4258..4291
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4342..4375
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        complement(4428..5639)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(4572..4591)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    5704..5821
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6119..6163)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6348..6581
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7074..7199
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7246..7426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7444..7642)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7469..7489)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7644..8023
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8195..8214
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8289..8393
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8394..8462,8463..9254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8612..8631)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9425..10013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9914..9933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10167..10184
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
      241 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac
      301 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      361 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat
      421 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
      481 catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca
      541 cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct
      601 cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg
      661 accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg
      721 gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg
      781 cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg
      841 ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt
      901 agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa
      961 cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc
     1021 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc
     1081 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     1141 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     1201 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     1261 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     1321 acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc
     1381 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     1441 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     1501 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     1561 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     1621 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     1681 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     1741 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc
     1801 cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta
     1861 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     1921 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     1981 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     2041 gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac
     2101 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     2161 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     2221 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagt ccggatgcag
     2281 ctctcgggcc atgtgatgaa atgctaggcg gctgtcaaac ctccactcta atacttctct
     2341 ctccgggtca tccatcccat gcaggctcac agggtgtaac aagcgggtgt tctctccttc
     2401 attggcttct tctaccttct cttgctcaac tggtactagc ttgtagcacc atccaaaggt
     2461 cagtggatat ctgatccctg gccctggtgt gtagttctgc caatcaggga agtagccttg
     2521 tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc
     2581 cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg
     2641 tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt gtgcttctag
     2701 ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc
     2761 taggtctcga ggtcgacggt atcgatgcgg ggaggcggcc caaagggaga tccgactcgt
     2821 ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg
     2881 aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca
     2941 ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga
     3001 attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag
     3061 ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt
     3121 ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg
     3181 cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat acaaggagga
     3241 gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc
     3301 acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta
     3361 atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta tagcatacat
     3421 tatacgaagt tattctttgc accattctaa agaataacag tgataatttc tgggttaagg
     3481 caaataactt cgtataggat actttatacg aagttatgct agcgccacca tggtgagcaa
     3541 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3601 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3661 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3721 cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     3781 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3841 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3901 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3961 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4021 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     4081 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     4141 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     4201 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag gcgcgccata
     4261 acttcgtata atgtatgcta tacgaagtta ttaagaggtt tcatattgct aatagcagct
     4321 acaatccagc taccattctg cataacttcg tataaagtat cctatacgaa gttattccgg
     4381 agtcgactct agaggatcct ctagagtcga cctgcagccc aagcttcgtc taacaaaaaa
     4441 gccaaaaacg gccagaattt agcggacaat ttactagtct aacactgaaa attacatatt
     4501 gacccaaatg attacatttc aaaaggtgcc taaaaaactt cacaaaacac actcgccaac
     4561 cccgagcgca tagttcaaaa ccggagcttc agctacttaa gaagataggt acataaaacc
     4621 gaccaaagaa actgacgcct cacttatccc tcccctcacc agaggtccgg cgcctgtcga
     4681 ttcaggagag cctaccctag gcccgaaccc tgcgtcctgc gacggagaaa agcctaccgc
     4741 acacctaccg gcaggtggcc ccaccctgca ttataagcca acagaacggg tgacgtcacg
     4801 acacgacgag ggcgcgcgct cccaaaggta cgggtgcact gcccaacggc accgccataa
     4861 ctgccgcccc cgcaacagac gacaaaccga gttctccagt cagtgacaaa cttcacgtca
     4921 gggtccccag atggtgcccc agcccatctc acccgaataa gagctttccc gcattagcga
     4981 aggcctcaag accttgggtt cttgccgccc accatgcccc ccaccttgtt tcaacgacct
     5041 cacagcccgc ctcacaagcg tcttccattc aagactcggg aacagccgcc attttgctgc
     5101 gctcccccca acccccagtt cagggcaacc ttgctcgcgg acccagacta cagcccttgg
     5161 cggtctctcc acacgcttcc gtcccaccga gcggcccggc ggccacgaaa gccccggcca
     5221 gcccagcagc ccgctactca ccaagtgacg atcacagcga tccacaaaca agaaccgcga
     5281 cccaaatccc ggctgcgacg gaactagctg tgccacaccc ggcgcgtcct tatataatca
     5341 tcggcgttca ccgccccacg gagatccctc cgcagaatcg ccgagaaggg actacttttc
     5401 ctcgcctgtt ccgctctctg gaaagaaaac cagtgcccta gagtcaccca agtcccgtcc
     5461 taaaatgtcc ttctgctgat actggggttc taaggccgag tcttatgagc agcgggccgc
     5521 tgtcctgagc gtccgggcgg aaggatcagg acgctcgctg cgcccttcgt ctgacgtggc
     5581 agcgctcgcc gtgaggaggg gggcgcccgc gggaggcgcc aaaacccggc gcggaggcca
     5641 gatctggagc cgacacgggt taattaacca aactggatct ctgctgtccc tgtaataaac
     5701 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     5761 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     5821 attgtggatg aatactgcca tttgtctgca gaattggcgc acgcagtgcc gatccgttca
     5881 ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc
     5941 tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc
     6001 taactctatt cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta
     6061 ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca
     6121 aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg
     6181 attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca
     6241 tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag
     6301 gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat
     6361 cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg
     6421 tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca
     6481 ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag
     6541 tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt
     6601 ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta
     6661 ctactttata tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc
     6721 tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc
     6781 ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt
     6841 gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc
     6901 tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat
     6961 cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct
     7021 ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc
     7081 tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt
     7141 cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc
     7201 tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc
     7261 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     7321 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     7381 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaac
     7441 gcgctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     7501 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     7561 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     7621 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     7681 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     7741 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     7801 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     7861 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     7921 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     7981 cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc
     8041 cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg
     8101 gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac
     8161 acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8221 gtgcaccata ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg
     8281 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8341 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8401 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8461 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8521 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8581 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8641 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8701 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8761 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8821 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8881 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8941 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9001 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9061 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9121 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9181 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9241 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9301 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9541 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10081 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10141 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
