LOCUS       sequence-169513-        3415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrate plasmid of Streptococcus pneumoniae, which can integrate 
            sgRNA targeting luc gene, which encode luciferase, into the locus 
            between amiF and treR. This vector can be used as the template for.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Liu X, Gallay C, Kjos M, Domenech A, Slager J, van Kessel SP, Knoops
            K, Sorg RA, Zhang JR, Veening JW
  TITLE     High-throughput CRISPRi phenotyping identifies new essential genes 
            in Streptococcus pneumoniae.
  JOURNAL   Mol Syst Biol. 2017 May 10;13(5):931. doi: 10.15252/msb.20167449.
  PUBMED    28490437
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2017-05-10"; volume: "13(5):931. doi"; pages: " 
            10.15252/msb.20167449"
COMMENT     Sequence Label: pPEPX-P3-sgRNAluc
FEATURES             Location/Qualifiers
     source          1..3415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      8..596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     497..516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2438..2509
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2525..2552
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
       61 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      121 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
      181 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      241 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      301 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      361 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      421 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      481 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      541 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      601 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      661 ttcctgcgtt atcccgcggc cgcagttaaa gaacttggac tgcgctctaa aaccaacgtg
      721 gtcagtctgg acaagattat tattgataaa aaatcctcac tgataaccgg tttcccagag
      781 tttcggatgg tttggaaggt ggtccgccag cgtgtggtgg atgatctggt atccgttctg
      841 gatacggact atctggatat ggaactaatc ccaaatctca ctcgccaaat cgctgagcag
      901 tctatctatt cttatataga aaatggcctc aaactcctta ttgattatgc tcagaaggaa
      961 atcaccattg accactcaag cgaccgagac aagattctca tggacattgg caaagaccct
     1021 tatgtcgttt cgattaaatc aaaagtctat ctccaagacg gacgccaatt tcagtttacc
     1081 gaaagtcgcc ataagttaga aaaatttaga tttgtagatt ttgcaaaacg caagaaataa
     1141 aagactgaga caccagatca tggctagaaa gcttctagca aaaaactgga cggagtcccg
     1201 tccagaaaag taagcacttg ttattgcaat aaaattagcc taattgagag aagtttctat
     1261 agaatttttc atatacttaa cgagtgcttt cacctttgaa tatagtcctt cccacttatc
     1321 atcacactct ccccgatagc cttttctagc tatatccagt aaagttacat gctctttagg
     1381 taaaagaggt atagcccatt cagcagcgac atctttcgag gtaatttcac cagtagtcac
     1441 tgtttgccac attcgagcta gggttaaaat tacattacgc tcatcacctt ttatcccctc
     1501 aattagttct ggcaaagaat ccttaattgc tcttcgaata tctgtcaaag gtacggagac
     1561 aagtatactt gaagaatcag gaccaaatag agaaatacta ttctttcttg cttgtgctaa
     1621 aacaatagcc aaatcaggat catagcttgg ttcctgaatt tgtccattct caaattcacc
     1681 cctgagccac tcaccgtata taaattctct ttttggagga tattgccaag ggacaacttc
     1741 actcctattt ataaccgtaa cttcaagtgg tctaacagaa tccgtatttc caatccttcc
     1801 tgatatagtc attagtcttt ctgttagttt ttttcgagtt aattgaggta aactatgatt
     1861 cacgacgact agaacatcta catcgctgtt aatgcgtaaa ccaccattta ctgctgaacc
     1921 aaatagatat actccaacta ttgaacttcc aaataaatct tttacgattt ttaatgtttg
     1981 aatcgcttga tttggtattt ttccgttaat caaattgctc atgatttcac ctcgttgatt
     2041 atgttcatat aaagtttata ttgatactca atttacttac cctagattgg acatatactt
     2101 aaattactgt tcaataaagc tgaccgttag cgtttaagta catcctttca caatttgtct
     2161 acagaacagt actggcctac ctagtatagc attttgtgaa gtttttttct agtccaagct
     2221 cacaaaaatc caaaggcatg cggcaaagca ctcaaaagtt taccttatgg gtgctttttt
     2281 cgtgcttttt tgaaaagaca aaaaaaagaa ccttgccaag caagattctt ttgatagggt
     2341 ggtctccgat cgtgtactgc agaaaactag tcgcaaaaaa ccccgcttcg gcggggtttt
     2401 ttcgctctag acggtgatca acacgctagc caggcatcaa ataaaacgaa aggctcagtc
     2461 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2521 cactggctca ccttcgggtg ggcctttctg cgtttataga attcggtcga cagatctcca
     2581 ttctacagtt tattcttgac attgcactgt ccccctggta taataactat atagaggata
     2641 gaatggcgcc gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc
     2701 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttgaagctt gggcccgaac
     2761 aaaaactcat ggatccctcc agtaactcga gaaaaaaaaa ccgcgcccct gacagggcgc
     2821 ggtttttttt taagtggaca cctacgtatg gtaactgtac aggtcgctga aacttctatc
     2881 gtcaagaaga atcaccaaat ccctcgtatc atcaaccaaa agattgctca aaagttaatt
     2941 gaaaagattt ctatgactga tattgcccat cagctttcca tctcaacttc aactgttatt
     3001 cgtaagctca atgattttca ctttaaacat gatttttcgc gtcttcctga gattatgtct
     3061 tgggatgagt atgcttttac aaagggaaag atgagcttca ttgcgcaaga ttttaacaat
     3121 ctcaatatca tcactgttct tgagggaaga acacaagcta tcattcgaaa tcactttctt
     3181 cgctacgata gagttgtccg atgtcgcgtc aaaattatta ctatggatat gtttagccct
     3241 tactatgact tggctaaaca gcttcgcttt caaatttcta ggctcaggct gaaacagtct
     3301 cccagactgt ttcactcccg aatgctaaaa tcgttcttga tcgctttcac attggacgtc
     3361 ctgaccatgg agtcaagtgt aaggcactgg taccaccccg tagaaaagat caaag
//
