LOCUS       sequence-169538-        7675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SOBIR1 split YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Postma J, Liebrand TW, Bi G, Evrard A, Bye RR, Mbengue M, Kuhn H, 
            Joosten MH, Robatzek S
  TITLE     Avr4 promotes Cf-4 receptor-like protein association with the 
            BAK1/SERK3 receptor-like kinase to initiate receptor endocytosis and
            plant immunity.
  JOURNAL   New Phytol. 2016 Apr;210(2):627-42. doi: 10.1111/nph.13802. Epub 
            2016 Jan 14.
  PUBMED    26765243
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.13802";
            journalName: "New Phytol"; date: "2016-04"; volume: "210"; issue: 
            "2"; pages: "627-42"
COMMENT     Sequence Label: p35S::SISOBIR1-YFPn
FEATURES             Location/Qualifiers
     source          1..7675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     347..363
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     348..364
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    380..404
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2316..2340)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2348..2350,2351..2353,2354..2812)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(2393..2414)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2654..2673)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    3104..3128
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3240..3949
                     /label=oriV
                     /note="incP origin of replication"
     promoter        4022..4113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4114..4182,4183..4974)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4332..4351)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5145..5733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5634..5653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    6045..6069
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6163..6415)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6435..6986)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7028..7207)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      181 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga ggtcgacggt
      361 atcgataagc ttcccatcaa caagtttgta caaaaaagca ggctccgcgg ccgccccctt
      421 caccatgact tcgaatatcc acttttttct tttatacgtt gtgagccttt tcctttttgt
      481 tcaagcaaga ttgaatcttt atccaccaga tcatgctgca ctcttgcttg tacaaaagga
      541 cttaggcatc atttctgtta acaacccgtg tacgctcgca ggaatatcgt gcgagcgtag
      601 accgggtaac acaacacaag ttgtgagagt tacccgtatt gtattcagat ccaacggatt
      661 aaagggaact ttgtcttctg ctattggcaa actcactgag ctcaaagagc tttctctttc
      721 cgacaatcaa ctatctgaac aaatccccgt tcagattatt gattgtcgga agttagagat
      781 tcttcaactt caaaggaaca gattttctgg gaagattcca tccgaattgt cagctttaaa
      841 ccgtttaagg atagttgact tttcatcgaa tgagttttcg gggaatcttg atttcttgaa
      901 gtactttcct aatctggaaa aactgtctct ggctgataat atgttcactg gaaaaatacc
      961 attttcattg aaatctttcc gaaatcttcg ttttctcaac atttcaggga atagtttcct
     1021 tgaaggtcca gtacctgtca tgagccaaat tgagcattta tcagcagatt tgaatcgaaa
     1081 aaatggcgtt cctaaacgtt acattctagc tgagaattca acaaggatat ctgcaatggg
     1141 acctgcttcg gttccagctc cagctccagc acctgtcaat cgtgttgtac cggcgatgca
     1201 taaacgtaag aacaagaaaa ggaagttacg atcttggttt cttggtttcc tagctggaac
     1261 ttttgctggg ggtatatctg ctgtgctctg ttccttgcta ttcaagatgg taatgttctt
     1321 cgtaagaaga gggaataacg attcaagttt aacgatatac agtccactga tcaagaaagc
     1381 cgaggacttg gctttcttag agaaggaaga tggagtggca tcgcttgaaa tgattggaaa
     1441 aggtggctgt ggagaagttt atagagcaga gctaccgggg agtaatggga agattatagc
     1501 tataaagaag attatccaat ccccaatgga tgctgcagag atcacggagg aagatactaa
     1561 ggcgttgaac aagaaaatgc gtcaagttaa atcagaaatt caaattgtag gtcaaatcag
     1621 acaccggaat ctgcttccat tactcgcgca tatgccaagg ccagactgtc attacttggt
     1681 ctacgaatac atgaaaaatg ggagtttaca ggatatcctg cagcaagtca cagaaggcac
     1741 aagggaatta gattggttgg gacgacacag aattgcagcg ggggtagctg ctggtctcga
     1801 gtatctccat ataaaccata ctcaacgcat aattcacaga gatttaaagc cagcaaatat
     1861 cctacttgat gatgacatgg aagctcgagt tgctgatttt ggccttgcaa aggcagttcc
     1921 agatgctcat acacatatca ccacttcaaa tgttgcagga actgttggat ttattgcacc
     1981 agaatattat cagacactga agtttacaga taagtgtgat atatacagct ttggtgtggt
     2041 gctagctgtg ttggttatcg gtaagggtcc atcggatgat tttttccaac atacttctga
     2101 gatgagttta gttaagtggc tgaggaatgt aatgacttct gacgatccta aaatagcaat
     2161 tgatcctaag cttataggaa atggatacga cgaacaaatg cttttggttc tcaagatagc
     2221 ttgcttttgt actctcgaca atccaaagga gaggcctaac agtaaggatg ttaggtgcat
     2281 gttaactcag atcaagcata agggtgggcg cgccgaccca gctttcttgt acaaagtggt
     2341 tgatgggatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2401 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2461 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     2521 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
     2581 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2641 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2701 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     2761 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg cctaatctag
     2821 agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa
     2881 taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg
     2941 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     3001 atttctaatt cctaaaacca aaatccagtg accgggcggc cgccaccgcg gtggaggggg
     3061 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
     3121 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     3181 aaggtttatc cgttcgtcca tttgtatgtg tacatcaccg acgagcaagg caagaccgag
     3241 cgcctttccg acgctcaccg ggctggttgc cctcgccgct gggctggcgg ccgtctatgg
     3301 ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccggc cgccggcgtt
     3361 gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt
     3421 gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg
     3481 cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc
     3541 cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg
     3601 cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat
     3661 gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca
     3721 agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa
     3781 tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga
     3841 aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc
     3901 caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggcagtc
     3961 cttgccattg ccgggatcgg ggcagtaacg ggatgggcga tcagcccgaa agctacccct
     4021 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4081 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4141 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4201 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4261 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4321 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4381 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4441 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4501 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4561 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4621 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4681 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4741 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4801 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4861 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4921 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4981 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5041 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5281 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5341 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5401 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5461 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5761 ttcctggcct tttgctggcc ttttgctcac atggactcta gctagaggat cacaggcagc
     5821 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
     5881 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
     5941 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
     6001 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
     6061 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgatc
     6121 gaatactaac gtctctacca gatatcagct tgcatgccgg tcgatctagt aacatagatg
     6181 acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt
     6241 aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt
     6301 acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa
     6361 gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctgctt
     6421 gactctaggg gtcatcagat ttcggtgacg ggcaggaccg gacggggcgg caccggcagg
     6481 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
     6541 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
     6601 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
     6661 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
     6721 gactcggccg tccagtcgta ggcgttgcgt gccttccagg gacccgcgta ggcgatgccg
     6781 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
     6841 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
     6901 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
     6961 tcggccgggc gtcgttctgg gctcatggta gatcccctcg atcgagttga gagtgaatat
     7021 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
     7081 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
     7141 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt
     7201 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa
     7261 cgtgactccc ttaattctcc gctcatgatc agattgtcgt ttcccgcctt cggtttgggc
     7321 gcgcccggtt cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga
     7381 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
     7441 gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
     7501 tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa
     7561 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atacatggtg gagcacgaca
     7621 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccagagg gctat
//
