LOCUS       sequence-169539-       10528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  exogenous AR expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Olsen JR, Azeem W, Hellem MR, Marvyin K, Hua Y, Qu Y, Li L, Lin B, 
            Ke X, Oyan AM, Kalland K
  TITLE     Context dependent regulatory patterns of the androgen receptor and 
            androgen receptor target genes.
  JOURNAL   BMC Cancer. 2016 Jul 4;16:377. doi: 10.1186/s12885-016-2453-4.
  PUBMED    27378372
REFERENCE   2  (bases 1 to 10528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Cancer."; date: "2016-07-4"; volume: "16:377. doi"; pages: " 
            10.1186/s12885-016-2453-4"
COMMENT     Sequence Label: pLENTI6.3/AR-GC-E2325
FEATURES             Location/Qualifiers
     source          1..10528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    321..345
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3142..3166)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3219..3260
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3284..3872
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3337..3357)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3755..3766)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3887..4203
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4054..4189
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4251..4298
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4317..4715
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4802..5035
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5107..5228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5144..5163)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5198..5217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5268..5403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5423..5442)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5424..5442)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5452..5469)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5452..5468)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5461..5483)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5610..6065
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5697..5716)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5907..5928
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6091..6195
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6196..6264,6265..7056)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6414..6433)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7227..7815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7716..7735
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7969..7986
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8103..8124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8139..8144,8145..8162,8163..8169)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8177..8193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8182..8204
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8201..8217
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8201..8217
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8236..8256
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8238..8256
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8284..8510
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8511..8691
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8738..8863
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9356..9589
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9774..9818
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10085..10202
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10225..10528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctaga ggatccacta gtccagtgtg
      301 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggcttaagg agtttaaaca
      361 ccatggaagt gcagttaggg ctgggaaggg tctaccctcg gccgccgtcc aagacctacc
      421 gaggagcttt ccagaatctg ttccagagcg tgcgcgaagt gatccagaac ccgggcccca
      481 ggcacccaga ggccgcgagc gcagcacctc ccggcgccag tttgctgctg ctgcagcagc
      541 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
      601 aagagactag ccccaggcag cagcagcagc agcagggtga ggatggttct ccccaagccc
      661 atcgtagagg ccccacaggc tacctggtcc tggatgagga acagcaacct tcacagccgc
      721 agtcggccct ggagtgccac cccgagagag gttgcgtccc agagcctgga gccgccgtgg
      781 ccgccagcaa ggggctgccg cagcagctgc cagcacctcc ggacgaggat gactcagctg
      841 ccccatccac gttgtccctg ctgggcccca ctttccccgg cttaagcagc tgctccgctg
      901 accttaaaga catcctgagc gaggccagca ccatgcaact ccttcagcaa cagcagcagg
      961 aagcagtatc cgaaggcagc agcagcggga gagcgaggga ggcctcgggg gctcccactt
     1021 cctccaagga caattactta gggggcactt cgaccatttc tgacaacgcc aaggagttgt
     1081 gtaaggcagt gtcggtgtcc atgggcctgg gtgtggaggc gttggagcat ctgagtccag
     1141 gggaacagct tcggggggat tgcatgtacg ccccactttt gggagttcca cccgctgtgc
     1201 gtcccactcc ttgtgcccca ttggccgaat gcaaaggttc tctgctagac gacagcgcag
     1261 gcaagagcac tgaagatact gctgagtatt cccctttcaa gggaggttac accaaagggc
     1321 tagaaggcga gagcctaggc tgctctggca gcgctgcagc agggagctcc gggacacttg
     1381 aactgccgtc taccctgtct ctctacaagt ccggagcact ggacgaggca gctgcgtacc
     1441 agagtcgcga ctactacaac tttccactgg ctctggccgg accgccgccc cctccgccgc
     1501 ctccccatcc ccacgctcgc atcaagctgg agaacccgct ggactacggc agcgcctggg
     1561 cggctgcggc ggcgcagtgc cgctatgggg acctggcgag cctgcatggc gcgggtgcag
     1621 cgggacccgg ttctgggtca ccctcagccg ccgcttcctc atcctggcac actctcttca
     1681 cagccgaaga aggccagttg tatggaccgt gtggtggtgg tgggggtggt ggcggcggcg
     1741 gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg cgaggcggga gctgtagccc
     1801 cctacggcta cactcggccc cctcaggggc tggcgggcca ggaaagcgac ttcaccgcac
     1861 ctgatgtgtg gtaccctggc ggcatggtga gcagagtgcc ctatcccagt cccacttgtg
     1921 tcaaaagcga aatgggcccc tggatggata gctactccgg accttacggg gacatgcgtt
     1981 tggagactgc cagggaccat gttttgccca ttgactatta ctttccaccc cagaagacct
     2041 gcctgatctg tggagatgaa gcttctgggt gtcactatgg agctctcaca tgtggaagct
     2101 gcaaggtctt cttcaaaaga gccgctgaag ggaaacagaa gtacctgtgc gccagcagaa
     2161 atgattgcac tattgataaa ttccgaagga aaaattgtcc atcttgtcgt cttcggaaat
     2221 gttatgaagc agggatgact ctgggagccc ggaagctgaa gaaacttggt aatctgaaac
     2281 tacaggagga aggagaggct tccagcacca ccagccccac tgaggagaca acccagaagc
     2341 tgacagtgtc acacattgaa ggctatgaat gtcagcccat ctttctgaat gtcctggaag
     2401 ccattgagcc aggtgtagtg tgtgctggac acgacaacaa ccagcccgac tcctttgcag
     2461 ccttgctctc tagcctcaat gaactgggag agagacagct tgtacacgtg gtcaagtggg
     2521 ccaaggcctt gcctggcttc cgcaacttac acgtggacga ccagatggct gtcattcagt
     2581 actcctggat ggggctcatg gtgtttgcca tgggctggcg atccttcacc aatgtcaact
     2641 ccaggatgct ctacttcgcc cctgatctgg ttttcaatga gtaccgcatg cacaagtccc
     2701 ggatgtacag ccagtgtgtc cgaatgaggc acctctctca agagtttgga tggctccaaa
     2761 tcacccccca ggaattcctg tgcatgaaag cactgctact cttcagcatt attccagtgg
     2821 atgggctgaa aaatcaaaaa ttctttgatg aacttcgaat gaactacatc aaggaactcg
     2881 atcgtatcat tgcatgcaaa agaaaaaatc ccacatcctg ctcaagacgc ttctaccagc
     2941 tcaccaagct cctggactcc gtgcagccta ttgcgagaga gctgcatcag ttcacttttg
     3001 acctgctaat caagtcacac atggtgagcg tggactttcc ggaaatgatg gcagagatca
     3061 tctctgtgca agtgcccaag atcctttctg ggaaagtcaa gcccatctat ttccacaccc
     3121 agtagctcga gtgcggccgc aacccagctt tcttgtacaa agtggttgat atccagcaca
     3181 gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     3241 tcctcggtct cgattctacg cgtaccggtt agtaatgatc gacaatcaac ctctggatta
     3301 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3361 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3421 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3481 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3541 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3601 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3661 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     3721 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3781 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3841 gagtcggatc tccctttggg ccgcctcccc gcctggcgat ggtaccggtg tggaaagtcc
     3901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     3961 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4021 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4081 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4141 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4201 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     4261 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     4321 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     4381 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     4441 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     4501 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     4561 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     4621 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     4681 aattgctgcc ctctggttat gtgtgggagg gctaagcaca attcgagctc ggtaccttta
     4741 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4801 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     4861 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4921 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4981 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5041 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5101 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5161 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5221 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     5281 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5341 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5401 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     5461 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5521 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5581 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     5641 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5701 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5761 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5821 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5881 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5941 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6001 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6061 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6121 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6181 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6241 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6301 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6361 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6421 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6481 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6541 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6601 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6661 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6721 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6781 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6841 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6901 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6961 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7021 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7081 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7141 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7201 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7261 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7321 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7381 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7441 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7501 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7561 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7621 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7681 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7741 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7801 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7861 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7921 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7981 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8041 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     8101 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8161 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     8221 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     8281 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     8341 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     8401 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     8461 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     8521 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8581 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8641 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     8701 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     8761 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     8821 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     8881 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     8941 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     9001 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     9061 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     9121 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     9181 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     9241 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     9301 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     9361 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     9421 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     9481 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     9541 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     9601 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     9661 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     9721 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     9781 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     9841 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     9901 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9961 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
    10021 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
    10081 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    10141 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    10201 ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
    10261 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
    10321 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
    10381 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
    10441 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
    10501 ctacgtatta gtcatcgcta ttaccatg
//
