LOCUS       sequence-169549-        8248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Eggenschwiler R, Moslem M, Fraguas MS, Galla M, Papp O, Naujock M, 
            Fonfara I, Gensch I, Wahner A, Beh-Pajooh A, Mussolino C, Tauscher 
            M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, 
            Cathomen T, Cantz T
  TITLE     Improved bi-allelic modification of a transcriptionally silent locus
            in patient-derived iPSC by Cas9 nickase.
  JOURNAL   Sci Rep. 2016 Dec 2;6:38198. doi: 10.1038/srep38198.
  PUBMED    27910942
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-12-2"; volume: "6:38198. doi"; pages: " 
            10.1038/srep38198"
COMMENT     Sequence Label: AAT-PB-CG2APtk
FEATURES             Location/Qualifiers
     source          1..8248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(38..59)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(299..318)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             719..772
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             776..1372
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(776..795)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1272..1292
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1379..2374
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(2396..2413)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2402..2626
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(2712..2736)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     repeat_region   2918..2980
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     4003..4019
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4053..4069)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4054..4070)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4099..4119)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4099..4117)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4138..4154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4138..4154)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4151..4173)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4162..4178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4186..4192,4193..4210,4211..4216))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4231..4252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4369..4386)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4540..5128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4620..4639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5299..6090,6091..6159))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5922..5941
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6160..6264)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6290..6745)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6427..6448)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6639..6658
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6872..6894
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6886..6903
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6887..6903
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6913..6932
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6913..6931
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   7967..8001
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
       61 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      121 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      181 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      241 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      301 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      361 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      421 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      481 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      541 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      601 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      661 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gctccggaga
      721 gggccggggc tctctgctga cctgtggcga cgtggaggag aaccccggcc cctccatgac
      781 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
      841 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
      901 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
      961 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     1021 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     1081 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     1141 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     1201 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     1261 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     1321 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccggatccat
     1381 gcccacgcta ctgcgggttt atatagacgg tcctcacggg atggggaaaa ccaccaccac
     1441 gcaactgctg gtggccctgg gttcgcgcga cgatatcgtc tacgtacccg agccgatgac
     1501 ttactggcag gtgctggggg cttccgagac aatcgcgaac atctacacca cacaacaccg
     1561 cctcgaccag ggtgagatat cggccgggga cgcggcggtg gtaatgacaa gcgcccagat
     1621 aacaatgggc atgccttatg ccgtgaccga cgccgttctg gctcctcata tcggggggga
     1681 ggctgggagc tcacatgccc cgcccccggc cctcaccctc atcttcgacc gccatcccat
     1741 cgccgccctc ctgtgctacc cggccgcgcg ataccttatg ggcagcatga ccccccaggc
     1801 cgtgctggcg ttcgtggccc tcatcccgcc gaccttgccc ggcacaaaca tcgtgttggg
     1861 ggcccttccg gaggacagac acatcgaccg cctggccaaa cgccagcgcc ccggcgagcg
     1921 gcttgacctg gctatgctgg ccgcgattcg ccgcgtttac gggctgcttg ccaatacggt
     1981 gcggtatctg cagggcggcg ggtcgtggcg ggaggattgg ggacagcttt cggggacggc
     2041 cgtgccgccc cagggtgccg agccccagag caacgcgggc ccacgacccc atatcgggga
     2101 cacgttattt accctgtttc gggcccccga gttgctggcc cccaacggcg acctgtacaa
     2161 cgtgtttgcc tgggccttgg acgtcttggc caaacgcctc cgtcccatgc acgtctttat
     2221 cctggattac gaccaatcgc ccgccggctg ccgggacgcc ctgctgcaac ttacctccgg
     2281 gatggtccag acccacgtca ccacccccgg ctccataccg acgatctgcg acctggcgcg
     2341 cacgtttgcc cgggagatgg gggaggctaa ctgagctcta gagctcgctg atcagcctcg
     2401 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2461 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2521 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2581 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     2641 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgcactc
     2701 gagatatcta gacccagctt tcttgtacaa agtggtacta gttaaaagtt ttgttacttt
     2761 atagaagaaa ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt
     2821 ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa
     2881 ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat
     2941 tatctttaac gtacgtcaca atatgattat ctttctaggg ttaaccatcg acgagaaagg
     3001 gactgaagct gctggggcca tgtttttaga ggccataccc atgtctatcc cccccgaggt
     3061 caagttcaac aaaccctttg tcttcttaat gattgaacaa aataccaagt ctcccctctt
     3121 catgggaaaa gtggtgaatc ccacccaaaa ataactgcct ctcgctcctc aacccctccc
     3181 ctccatccct ggccccctcc ctggatgaca ttaaagaagg gttgagctgg tccctgcctg
     3241 catgtgactg taaatccctc ccatgttttc tctgagtctc cctttgcctg ctgaggctgt
     3301 atgtgggctc caggtaacag tgctgtcttc gggccccctg aactgtgttc atggagcatc
     3361 tggctgggta ggcacatgct gggcttgaat ccagggggga ctgaatcctc agcttacgga
     3421 cctgggccca tctgtttctg gagggctcca gtcttccttg tcctgtcttt gagtccccaa
     3481 gaaggaatca caggggagga accagatacc agccatgacc ccaggctcca ccaagcatct
     3541 tcatgtcccc ctgctcatcc cccactcccc cccacccaga gttgctcatc ctgccagggc
     3601 tggctgtgcc caccccaagg ctgccctcct ggggacccca gaactgcctg atcgtgccgt
     3661 ggcccagttt tgtggcatct gcagcaacac aagagagagg acaatgtcct cctcttgacc
     3721 cgctgtcacc taaccagact cgggccctgc acctctcagg cacttctgga aaatgactga
     3781 ggcagattct tcctgaagcc cattctccat ggggcaacaa ggacacctat tctgtccttg
     3841 tccttccatc gctgccccag aaagcctcac atatctccgt ttagaatcag gtcccttctc
     3901 cccagatgaa gaggagggtc tctgctttgt tttctctatc tcctcctcag acttgaccag
     3961 gcccagcagg ccccagaaga ccattaatta aggccgggcc gctctagaac tagtggatcc
     4021 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
     4081 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     4141 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4201 gagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4261 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4321 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4381 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4441 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4501 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4561 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4621 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4681 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4741 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4801 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4861 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4921 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4981 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5041 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5101 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5161 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5221 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5281 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5341 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5401 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5461 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5521 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5581 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5641 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5701 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5761 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5821 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5881 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5941 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6001 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6061 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6121 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6181 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6241 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     6301 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     6361 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     6421 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     6481 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     6541 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     6601 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     6661 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     6721 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     6781 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     6841 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     6901 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg agctcgttta aacggcgcgc
     6961 ctctgcacga caggtctgcc agcttacatt tacccaaact gtccattact ggaacctatg
     7021 atctgaagag cgtcctgggt caactgggca tcactaaggt cttcagcaat ggggctgacc
     7081 tctccggggt cacagaggag gcacccctga agctctccaa ggtgagatca ccctgacgac
     7141 cttgttgcac cctggtatct gtagggaaga atgtgtgggg gctgcagctc tgtcctgagg
     7201 ctgaggaagg ggccgaggga aacaaatgaa gacccaggct gagctcctga agatgcccgt
     7261 gattcactga cacgggacgt ggtcaaacag caaagccagg caggggactg ctgtgcagct
     7321 ggcactttcg gggcctccct tgaggttgtg tcactgaccc tgaatttcaa ctttgcccaa
     7381 gaccttctag acattgggcc ttgatttatc catactgaca cagaaaggtt tgggctaagt
     7441 tgtttcaaag gaatttctga ctccttcgat ctgtgagatt tggtgtctga attaatgaat
     7501 gatttcagct aaagatgaca cttattttgg aaaactaaag gcgaccaatg aacaactgca
     7561 gttccatgaa tggctgcatt atcttggggt ctgggcactg tgaaggtcac tgccagggtc
     7621 cgtgtcctca aggagcttca agccgtgtac tagaaaggag agagccctgg aggcagacgt
     7681 ggagtgacga tgctcttccc tgttctgagt tgtgggtgca cctgagcagg gggagaggcg
     7741 cttgtcagga agatggacag aggggagcca gccccatcag ccaaagcctt gaggaggagc
     7801 aaggcctatg tgacagggag ggagaggatg tgcagggcca gggccgtcca gggggagtga
     7861 gcgcttcctg ggaggtgtcc acgtgagcct tgctcgaggc ctgggatcag ccttacaacg
     7921 tgtctctgct tctctcccct ccaggccgtg cataaggctg tgttaaccct agaaagatag
     7981 tctgcgtaaa attgacgcat gcattcttga aatattgctc tctctttcta aatagcgcga
     8041 atccgtcgct gtgcatttag gacatctcag tcgccgcttg gagctcccgt gaggcgtgct
     8101 tgtcaatgcg gtaagtgtca ctgattttga actataacga ccgcgtgagt caaaatgacg
     8161 catgattatc ttttacgtga cttttaagat ttaactcata cgataattat attgttattt
     8221 catgttctac ttacgtgata acttatta
//
