LOCUS       sequence-169551-       14375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLS2-GFP expresssion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14375)
  AUTHORS   Robatzek S, Chinchilla D, Boller T
  TITLE     Ligand-induced endocytosis of the pattern recognition receptor FLS2 
            in Arabidopsis.
  JOURNAL   Genes Dev. 2006 Mar 1;20(5):537-42.
  PUBMED    16510871
REFERENCE   2  (bases 1 to 14375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2006-03-1"; volume: "20(5)"; pages: "537-42"
COMMENT     Sequence Label: pCAMBIA-FLS2prom::FLS2-3xmyc-GFP
FEATURES             Location/Qualifiers
     source          1..14375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3613..3642
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3649..3678
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3685..3714
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(3730..3732,3733..3735,3736..4449)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3730..3732,3733..3735,3736..4446)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3775..3796)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4036..4055)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4383..4404
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4685..4702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4685..4701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4694..4716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4904..4928
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6229..6858
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7287..8360
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8426..8620
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8856..8878)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8964..9104
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9019..9038
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9119..9136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9290..9878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9370..9389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9965..10759)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11184..11208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11286..11460
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11517..12314)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11628..11647)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     12238..12257
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(12377..13053)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(12420..12440)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    13244..13265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13280..13285,13286..13303,13304..13310)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13318..13334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13323..13345
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13342..13358
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13342..13358
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaagttac tctcaaagac ctttttgata ttaactctca ccttcttctt ctttggcatt
       61 gcactagcga aacagagctt tgaaccagag atcgaagctt tgaaatcctt caagaatggt
      121 atttccaacg accctttagg agtattatca gattggacca tcatcggttc gttacgacac
      181 tgtaattgga ccggaatcac ctgcgatagt accggacatg tagtctcggt ttccttgctg
      241 gagaagcaac ttgaaggtgt cctgtctcca gccatagcga atctcaccta tctccaggtt
      301 cttgatctca cttcaaatag ttttaccggc aaaataccgg ctgaaatagg aaagttaacc
      361 gagcttaacc agcttattct gtacctaaac tatttctctg gttcgattcc ttctggaatc
      421 tgggagctta agaatatttt ctatcttgat cttagaaata atttgttgtc cggtgatgtt
      481 cctgaggaaa tctgcaaaac cagttctttg gtattgattg ggtttgatta caacaactta
      541 accgggaaaa taccagaatg cttaggagat ttggttcatc tccaaatgtt tgtagcagct
      601 ggtaaccatt taactggttc gattccggta tcaattggta ctctggctaa tttaacggat
      661 ttagacctga gtggtaacca gttaaccgga aaaataccga gagattttgg aaatctcttg
      721 aacttacagt ctctcgtttt aactgaaaac ttgttggaag gagatatacc agctgagatc
      781 ggaaactgct cgagcttggt ccaacttgag ctttacgata accagttaac cgggaaaata
      841 ccagctgaat tagggaattt ggttcagctg caagcactcc ggatatacaa gaacaaactt
      901 acttcttcaa ttccatcttc attgttccgg ttaactcagt taacccattt ggggttatca
      961 gaaaaccatt tggttggacc gatatcagaa gaaatcggtt ttcttgagtc acttgaagtc
     1021 ctcacacttc attccaacaa cttcacagga gagtttccac agtccatcac aaacttgagg
     1081 aacttgacag tcctaacggt ggggttcaat aatatttccg gtgagctccc ggcggatcta
     1141 gggcttctta caaaccttcg gaacctttca gcgcacgaca atcttcttac cggaccaata
     1201 ccttccagca taagtaactg caccggtctt aaactcctgg acctgtctca caaccaaatg
     1261 actggcgaga tcccgcgggg tttcggaagg atgaatctta cgttcatttc tattgggagg
     1321 aatcatttca ccggtgaaat tccagatgat atcttcaact gttcaaactt ggaaactctt
     1381 agtgtggcag ataacaactt aacaggaact ctcaagccat taattgggaa gcttcaaaaa
     1441 ctcaggattt tgcaagtttc atataactct ctcactggac cgattcctcg agaaatcggg
     1501 aatctgaaag atttgaatat cttgtacctt cactctaatg gtttcacagg gagaatcccg
     1561 agagagatgt cgaatctcac tctcctccag gggctaagga tgtattcaaa tgatcttgaa
     1621 ggtccaattc ctgaagaaat gtttgatatg aagctactct cagttcttga tctttccaac
     1681 aacaaattct caggtcaaat tcctgccttg ttctccaagc ttgaatcgct tacctacttg
     1741 agtcttcaag gaaacaaatt caacgggtct atccctgcaa gccttaagtc gctttcgctt
     1801 ctcaacacat tcgatatctc cgacaatctt ctcactggaa ccatccctgg agagctgtta
     1861 gcttctttga aaaacatgca gctttacctc aacttctcaa acaacttgtt gactggaacc
     1921 atcccaaagg agcttggaaa gcttgaaatg gttcaagaaa tcgacctttc aaacaatctc
     1981 ttttctgggt ctattccaag atctttacag gcctgcaaaa atgtgttcac actggatttt
     2041 tcgcagaaca atctctcggg tcatatacca gatgaagtct tccaaggcat ggatatgatc
     2101 ataagcttga acctttcaag gaacagtttc tctggagaaa tccctcagag cttcgggaac
     2161 atgacgcatt tggtctcctt ggatctctct agtaacaatc tcactggtga aattccagag
     2221 agtctcgcca atctttcgac tctgaaacat ctcaaactag cttcaaacaa cctcaaaggc
     2281 catgttcctg aatccggggt gttcaaaaac atcaacgcct ctgatctaat gggaaacaca
     2341 gatctctgtg gtagcaagaa gcctctcaag ccatgtacga tcaagcagaa gtcgagccac
     2401 ttctcgaaga gaaccagagt catcctgatt attcttggat cagccgcggc tcttcttctt
     2461 gtcctgcttc ttgttctgat tctaacctgt tgcaagaaaa aagaaaaaaa gattgaaaat
     2521 tcatcagagt cctcattacc ggatttggat tcagctctga aactgaagag atttgaacca
     2581 aaagagttgg agcaagcaac agattcattc aacagtgcca acatcattgg ctcaagcagc
     2641 ttaagcacag tgtacaaagg tcagctagaa gatgggacag tgattgcagt aaaagtattg
     2701 aatctaaagg aattctctgc agaatcagac aagtggttct acacagaagc taaaacattg
     2761 agccaactaa aacatcgaaa cctggtgaag atcttagggt ttgcgtggga aagcggcaaa
     2821 acgaaagctt tagtgcttcc atttatggag aatggaaact tggaggacac cattcacggc
     2881 tctgcagcac cgattgggtc gcttttagaa aaaatcgatc tttgtgttca tatcgcaagc
     2941 ggaatcgatt atcttcattc tggatatggt tttcccatcg ttcattgtga tctgaagcca
     3001 gctaatatac tccttgacag tgaccgcgtt gctcacgtaa gcgattttgg aactgctcgg
     3061 attctaggtt tccgcgaaga tggaagcacc acagcttcaa catcagcctt cgagggtaca
     3121 attggatact tagctccagg taaattactc acaacactta attttcaaaa ccctaattat
     3181 gccatcgact tacttgaaat acactcttat ttttcatgaa cagagtttgc ttatatgagg
     3241 aaagtgacaa caaaagccga tgtattcagc ttcgggatca taatgatgga gctgatgacg
     3301 aaacagagac caacttcgtt gaatgatgaa gattcacaag acatgacttt gcgccaattg
     3361 gtggagaaat cgattggaaa tggaagaaaa gggatggtta gggttcttga tatggaactc
     3421 ggggactcta ttgtttctct gaaacaggaa gaggctattg aagactttct gaagctttgt
     3481 ttgttctgta caagctctag acctgaagat cgacctgata tgaacgagat tcttacacat
     3541 ctgatgaaac ttagaggcaa agcgaattca tttcgagaag atcgtaacga ggatcgagag
     3601 gtgcatatgg gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta
     3661 atctccgagg aagacttgaa cggtgaacaa aaattaatct cagaagaaga cttgaacgga
     3721 gcggtcgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     3781 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     3841 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     3901 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     3961 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4021 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4081 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4141 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     4201 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     4261 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4321 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4381 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4441 tacaagtaag tcgacctgca ggcatgcccg ctgaaatcac cagtctctct ctacaaatct
     4501 atctctctct ataataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
     4561 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     4621 cttctatcaa taaaatttct aattcctaaa accaaaatcc aggggtacct taattaagct
     4681 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4741 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4801 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
     4861 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
     4921 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     4981 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     5041 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     5101 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     5161 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     5221 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     5281 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     5341 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     5401 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     5461 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     5521 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     5581 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     5641 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     5701 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     5761 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     5821 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     5881 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     5941 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     6001 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
     6061 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     6121 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     6181 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     6241 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     6301 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     6361 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     6421 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     6481 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     6541 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     6601 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     6661 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     6721 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     6781 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     6841 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     6901 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     6961 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     7021 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     7081 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     7141 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     7201 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     7261 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     7321 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     7381 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     7441 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     7501 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     7561 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     7621 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     7681 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     7741 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     7801 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     7861 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     7921 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     7981 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     8041 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     8101 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     8161 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     8221 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     8281 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     8341 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     8401 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     8461 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     8521 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     8581 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     8641 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     8701 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     8761 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     8821 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8881 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     8941 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     9001 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9061 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     9121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9721 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     9961 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
    10021 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    10081 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    10141 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    10201 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    10261 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    10321 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    10381 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    10441 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    10501 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    10561 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    10621 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    10681 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    10741 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    10801 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    10861 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    10921 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
    10981 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
    11041 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
    11101 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    11161 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
    11221 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
    11281 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
    11341 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
    11401 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
    11461 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag
    11521 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
    11581 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
    11641 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
    11701 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg
    11761 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
    11821 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
    11881 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
    11941 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
    12001 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
    12061 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
    12121 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
    12181 cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt ctgttgtgcc
    12241 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
    12301 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag
    12361 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
    12421 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
    12481 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
    12541 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
    12601 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
    12661 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
    12721 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
    12781 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg
    12841 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
    12901 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
    12961 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
    13021 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
    13081 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
    13141 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    13201 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    13261 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    13321 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgaagttgt
    13381 gaattgtgat cataggagta atatttctat ctgaacaaaa attgaatttt gttttgcttt
    13441 ttgttgaaag ataaataaaa tgtttggcag attgtttgta cagaacacaa cttgttgata
    13501 aaacaaattc catcaagaca gctaatgagc ttttgatagt cagagctttg aagacgtgtt
    13561 aatgaattaa actgattaga cctaaaacca aatatttaga aagaaaaaaa tatgataatt
    13621 gttatgttat ttaatcaata gtcaaatact atgttgagta ccaatcattt cagtttcaac
    13681 gattgctact cttttgacca ataacaaaaa ggtttttatt tttcatttct caaactccat
    13741 ttttgtttgc ttttagcata aaaacagcta gaatgtgagt ttcatatgta ttatatacta
    13801 taagaaatca tataaattaa cgactagaac atattgtttt cattctagtg gtatatctga
    13861 gtttgactag aacgtttgga ggcagattaa agtgatttcg ccttgtcaaa actactttgt
    13921 atatgttatc aatcaatcgc tcaaaactaa atcggaaaaa aactacataa ataagatatg
    13981 gaaaatgata ctaacacaaa ctagtaagta agtagtcact acatattctg gtcccatgaa
    14041 tatggcttac atagtacata cattgtgaga ttaaatgaat atgatatata atacaagcat
    14101 ataagcatgt gcatgactat gaagtgggaa tacatttagt gcctttatat gcaacttgac
    14161 agaattacca attatgtgaa tagtgaaact tatattgcgt cttggccttt tcacatccgt
    14221 gacatgatcc ctcaaatcta tatcttcttg cggcatatgc ttatatataa acatatgact
    14281 tcgtctataa atttatatcc agataattaa ttctctcata atatcttctc cacaatttca
    14341 actcttctaa agtctaaacc ggatccgtct aaacc
//
