LOCUS       sequence-169572-        5782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iDamID plasmid. To express transiently the c-Myc-tagged and 
            optimized E. coli Dam adenine methyltransferase fused to the 
            nuclear-localized mmGFP via flexylinker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Gutierrez-Triana JA, Mateo JL, Ibberson D, Ryu S, Wittbrodt J
  TITLE     iDamIDseq and iDEAR: An improved method and computational pipeline 
            to profile chromatin-binding proteins.
  JOURNAL   Development. 2016 Oct 5. pii: dev.139261.
  PUBMED    27707796
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2016 Oct 5. pii: dev.139261."
COMMENT     Sequence Label: pCS2+ cMyc-oDam_f_GFPoNLS
FEATURES             Location/Qualifiers
     source          1..5782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..1009
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1044..1062
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1044..1061
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1107..1136
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2070..2783
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2100..2128)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2723..2743
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    2843..2977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2867..2886)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2921..2940
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3090..3110)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3090..3108)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3129..3145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3129..3145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3142..3164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3153..3169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3177..3183,3184..3201,3202..3207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3222..3243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3360..3377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3531..4119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3611..3630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4290..5081,5082..5150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4913..4932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5151..5255)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5281..5736)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5418..5439)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5630..5649
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgggcctct tcgctattac gccagtcgac cgccaattca atatggcgta tatggactca
       61 tgccaattca atatggtgga tctggacctg tgccaattca atatggcgta tatggactcg
      121 tgccaattca atatggtgga tctggacccc agccaattca atatggcgga cttggcacca
      181 tgccaattca atatggcgga cctggcactg tgccaactgg ggaggggtct acttggcacg
      241 gtgccaagtt tgaggagggg tcttggccct gtgccaagtc cgccatattg aattggcatg
      301 gtgccaataa tggcggccat attggctata tgccaggatc aatatatagg caatatccaa
      361 tatggcccta tgccaatatg gctattggcc aggttcaata ctatgtattg gccctatgcc
      421 atatagtatt ccatatatgg gttttcctat tgacgtagat agcccctccc aatgggcggt
      481 cccatatacc atatatgggg cttcctaata ccgcccatag ccactccccc attgacgtca
      541 atggtctcta tatatggtct ttcctattga cgtcatatgg gcggtcctat tgacgtatat
      601 ggcgcctccc ccattgacgt caattacggt aaatggcccg cctggctcaa tgcccattga
      661 cgtcaatagg accacccacc attgacgtca atgggatggc tcattgccca ttcatatccg
      721 ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc cacttggcag tacatcaata
      781 tctattaata gtaacttggc aagtacatta ctattggaag tacgccaggg tacattggca
      841 gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat ggctgccaag tacatcccca
      901 ttgacgtcaa tggggagggg caatgacgca aatgggcgtt ccattgacgt aaatgggcgg
      961 taggcgtgcc taatgggagg tctatataag caatgctcgt ttagggaacc gccattctgc
     1021 ctggggacgt cggagcaagc ttgatttagg tgacactata gaatacaagc tacttgttct
     1081 ttttgcagga tccaccggtc aacatggagc aaaagctgat ctcggaggag gacctgggcg
     1141 gctcaggcaa gaagaacagg gccttcctga agtgggctgg cggaaagtac ccactgctgg
     1201 acgacatcaa gaggcacctg cccaagggcg agtgcctggt cgagcctttc gtgggagccg
     1261 gaagcgtgtt cctgaacacc gacttcagcc gctacatcct ggccgacatc aacagcgacc
     1321 tgatcagcct gtacaacatc gtgaagatga ggaccgacga gtacgtgcag gccgctcgcg
     1381 agctgttcgt gcccgagaca aactgcgccg aggtgtacta ccagttcaga gaagagttca
     1441 acaagagcca ggaccccttc cgcagggccg tgctgttcct gtacctgaac agatacggat
     1501 acaacggcgc ctgcagatac aacctgaggg gcgagttcaa cgtgcccttc ggaagataca
     1561 agaagcccta cttccccgag gccgagctgt accacttcgc cgagaaggcc cagaacgcat
     1621 tcttctactg cgagagctac gccgacagca tggccagggc cgacgacgcc tctgtggtgt
     1681 actgcgaccc tccctacgcc cctctgagcg ccaccgccaa cttcaccgcc taccacacca
     1741 acagcttcac cctggagcag caggcccacc tggccgagat cgctgaggga ctggtcgaga
     1801 ggcacatccc cgtgctgatc tccaaccacg acaccatgct gaccagagag tggtatcaga
     1861 gggccaagct gcacgtggtc aaagtgcgca gatccatcag cagcaacggc ggaaccagaa
     1921 agaaggtgga cgagctgctg gccctgtaca agcccggcgt ggtgtcccct gccaagaagg
     1981 ctagcctgag cggtggaggc ggttcaggcg gaggtggctc tggcggtggc ggatcgggag
     2041 gcggtggaag tgcagccgcg actagtacca tgagtaaagg agaagaactt ttcactggag
     2101 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gtacaaattt tctgtcagtg
     2161 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     2221 gaaaactacc tgttccatgg ccaacacttg tcactactct cacttatggt gttcaatgct
     2281 tttcaagata tccagatcat atgaagcggc acgacttctt caagagcgcc atgcctgagg
     2341 gatacgtgca ggagaggacc atcttcttca aggacgacgg gaactacaag acacgtgctg
     2401 aagtcaagtt tgagggagac accctcgtca acaggatcga gcttaaggga atcgatttca
     2461 aggaggacgg aaacatcctc ggccacaagt tggaatacaa ctacaactcc cacaacgtat
     2521 acatcatggc cgacaagcaa aagaacggca tcaaagccaa cttcaagacc cgccacaaca
     2581 tcgaagacgg cggcgtgcaa ctcgctgatc attatcaaca aaatactcca attggcgatg
     2641 accctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     2701 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacggctgct gggattacac
     2761 atggcatgga tgaactatac aaaccacctc ccaagaggcc caggctggac taatctagaa
     2821 ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca
     2881 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2941 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     3001 tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttcgcggccg cggcgccaat
     3061 gcattgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     3121 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3181 atacgagccg ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     3241 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     3301 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3961 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4201 tcaaaaagta tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4261 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4321 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4381 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4441 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4501 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4561 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4621 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4681 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4741 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4801 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4861 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4921 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4981 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5041 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5101 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5161 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5221 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5281 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     5341 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5401 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     5461 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     5521 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     5581 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     5641 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     5701 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     5761 actgttggga agggcgatcg gt
//
