LOCUS       sequence-169595-        7891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7891)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Alpha-5-Integrin-12
FEATURES             Location/Qualifiers
     source          1..7891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3467..3476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3473..3475,3476..3478,3479..4192)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3518..3539)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3779..3798)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4126..4147
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4315..4436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4352..4371)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4406..4425
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4443..4898)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4580..4601)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4792..4811
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4925..5029
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5031..5388
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5031..5222
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5054..5074)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5239..5374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5423..6217
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5423..6217
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5477..5496)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6087..6106
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6405..6424)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6449..6496
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6825..7413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7314..7333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7588..7891
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccacca tggggagccg
      301 gacgccagag tcccctctcc acgccgtgca gctgcgctgg ggcccccggc gccgaccccc
      361 gctgctgccg ctgctgttgc tgctgctgcc gccgccaccc agggtcgggg gcttcaactt
      421 agacgcggag gccccagcag tactctcggg gcccccgggc tccttcttcg gattctcagt
      481 ggagttttac cggccgggaa cagacggggt cagtgtgctg gtgggagcac ccaaggctaa
      541 taccagccag ccaggagtgc tgcagggtgg tgctgtctac ctctgtcctt ggggtgccag
      601 ccccacacag tgcaccccca ttgaatttga cagcaaaggc tctcggctcc tggagtcctc
      661 actgtccagc tcagagggag aggagcctgt ggagtacaag tccttgcagt ggttcggggc
      721 aacagttcga gcccatggct cctccatctt ggcatgcgct ccactgtaca gctggcgcac
      781 agagaaggag ccactgagcg accccgtggg cacctgctac ctctccacag ataacttcac
      841 ccgaattctg gagtatgcac cctgccgctc agatttcagc tgggcagcag gacagggtta
      901 ctgccaagga ggcttcagtg ccgagttcac caagactggc cgtgtggttt taggtggacc
      961 aggaagctat ttctggcaag gccagatcct gtctgccact caggagcaga ttgcagaatc
     1021 ttattacccc gagtacctga tcaacctggt tcaggggcag ctgcagactc gccaggccag
     1081 ttccatctat gatgacagct acctaggata ctctgtggct gttggtgaat tcagtggtga
     1141 tgacacagaa gactttgttg ctggtgtgcc caaagggaac ctcacttacg gctatgtcac
     1201 catccttaat ggctcagaca ttcgatccct ctacaacttc tcaggggaac agatggcctc
     1261 ctactttggc tatgcagtgg ccgccacaga cgtcaatggg gacgggctgg atgacttgct
     1321 ggtgggggca cccctgctca tggatcggac ccctgacggg cggcctcagg aggtgggcag
     1381 ggtctacgtc tacctgcagc acccagccgg catagagccc acgcccaccc ttaccctcac
     1441 tggccatgat gagtttggcc gatttggcag ctccttgacc cccctggggg acctggacca
     1501 ggatggctac aatgatgtgg ccatcggggc tccctttggt ggggagaccc agcagggagt
     1561 agtgtttgta tttcctgggg gcccaggagg gctgggctct aagccttccc aggttctgca
     1621 gcccctgtgg gcagccagcc acaccccaga cttctttggc tctgcccttc gaggaggccg
     1681 agacctggat ggcaatggat atcctgatct gattgtgggg tcctttggtg tggacaaggc
     1741 tgtggtatac aggggccgcc ccatcgtgtc cgctagtgcc tccctcacca tcttccccgc
     1801 catgttcaac ccagaggagc ggagctgcag cttagagggg aaccctgtgg cctgcatcaa
     1861 ccttagcttc tgcctcaatg cttctggaaa acacgttgct gactccattg gtttcacagt
     1921 ggaacttcag ctggactggc agaagcagaa gggaggggta cggcgggcac tgttcctggc
     1981 ctccaggcag gcaaccctga cccagaccct gctcatccag aatggggctc gagaggattg
     2041 cagagagatg aagatctacc tcaggaacga gtcagaattt cgagacaaac tctcgccgat
     2101 tcacatcgct ctcaacttct ccttggaccc ccaagcccca gtggacagcc acggcctcag
     2161 gccagcccta cattatcaga gcaagagccg gatagaggac aaggctcaga tcttgctgga
     2221 ctgtggagaa gacaacatct gtgtgcctga cctgcagctg gaagtgtttg gggagcagaa
     2281 ccatgtgtac ctgggtgaca agaatgccct gaacctcact ttccatgccc agaatgtggg
     2341 tgagggtggc gcctatgagg ctgagcttcg ggtcaccgcc cctccagagg ctgagtactc
     2401 aggactcgtc agacacccag ggaacttctc cagcctgagc tgtgactact ttgccgtgaa
     2461 ccagagccgc ctgctggtgt gtgacctggg caaccccatg aaggcaggag ccagtctgtg
     2521 gggtggcctt cggtttacag tccctcatct ccgggacact aagaaaacca tccagtttga
     2581 cttccagatc ctcagcaaga atctcaacaa ctcgcaaagc gacgtggttt cctttcggct
     2641 ctccgtggag gctcaggccc aggtcaccct gaacggtgtc tccaagcctg aggcagtgct
     2701 attcccagta agcgactggc atccccgaga ccagcctcag aaggaggagg acctgggacc
     2761 tgctgtccac catgtctatg agctcatcaa ccaaggcccc agctccatta gccagggtgt
     2821 gctggaactc agctgtcccc aggctctgga aggtcagcag ctcctatatg tgaccagagt
     2881 tacgggactc aactgcacca ccaatcaccc cattaaccca aagggcctgg agttggatcc
     2941 cgagggttcc ctgcaccacc agcaaaaacg ggaagctcca agccgcagct ctgcttcctc
     3001 gggacctcag atcctgaaat gcccggaggc tgagtgtttc aggctgcgct gtgagctcgg
     3061 gcccctgcac caacaagaga gccaaagtct gcagttgcat ttccgagtct gggccaagac
     3121 tttcttgcag cgggagcacc agccatttag cctgcagtgt gaggctgtgt acaaagccct
     3181 gaagatgccc taccgaatcc tgcctcggca gctgccccaa aaagagcgtc aggtggccac
     3241 agctgtgcaa tggaccaagg cagaaggcag ctatggcgtc ccactgtgga tcatcatcct
     3301 agccatcctg tttggcctcc tgctcctagg tctactcatc tacatcctct acaagcttgg
     3361 attcttcaaa cgctccctcc catatggcac cgccatggaa aaagctcagc tcaagcctcc
     3421 agccacctct gatgccacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
     3481 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3541 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3601 gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3661 caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     3721 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3781 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3841 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3901 gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     3961 ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta
     4021 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4081 ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4141 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     4201 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     4261 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     4321 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4381 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     4441 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     4501 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     4561 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     4621 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     4681 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4741 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     4801 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     4861 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     4921 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4981 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     5041 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5101 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5161 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5221 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5281 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5341 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     5401 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5461 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5521 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5581 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     5641 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5701 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5761 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5821 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5881 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5941 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6001 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6061 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6121 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6181 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6241 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6301 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6361 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     6421 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     6481 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     6541 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     6601 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     6661 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6721 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6781 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6841 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6901 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6961 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7021 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7081 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7141 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7201 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7261 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7321 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7381 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7441 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7501 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
     7561 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     7621 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     7681 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     7741 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     7801 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     7861 catctacgta ttagtcatcg ctattaccat g
//
