LOCUS       sequence-169608-       14366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLS2-mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14366)
  AUTHORS   Mbengue M, Bourdais G, Gervasi F, Beck M, Zhou J, Spallek T, Bartels
            S, Boller T, Ueda T, Kuhn H, Robatzek S
  TITLE     Clathrin-dependent endocytosis is required for immunity mediated by 
            pattern recognition receptor kinases.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):11034-9. doi: 
            10.1073/pnas.1606004113. Epub 2016 Sep 20.
  PUBMED    27651493
REFERENCE   2  (bases 1 to 14366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1606004113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-09-27- 27"; volume: "113"; issue: "39"; pages: "11034-9"
COMMENT     Sequence Label: pCAMBIA-FLS2-myc-mCherry
FEATURES             Location/Qualifiers
     source          1..14366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3613..3642
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3649..3678
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3685..3714
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3730..4440
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4143..4162
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4676..4693)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4676..4692)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4685..4707)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4895..4919
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6220..6849
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7278..8351
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8417..8611
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8847..8869)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8955..9095
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9010..9029
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9110..9127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9281..9869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9361..9380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9956..10750)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11175..11199
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11277..11451
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11508..12305)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11619..11638)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     12229..12248
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(12368..13044)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(12411..12431)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    13235..13256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13271..13276,13277..13294,13295..13301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13309..13325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13314..13336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13333..13349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13333..13349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaagttac tctcaaagac ctttttgata ttaactctca ccttcttctt ctttggcatt
       61 gcactagcga aacagagctt tgaaccagag atcgaagctt tgaaatcctt caagaatggt
      121 atttccaacg accctttagg agtattatca gattggacca tcatcggttc gttacgacac
      181 tgtaattgga ccggaatcac ctgcgatagt accggacatg tagtctcggt ttccttgctg
      241 gagaagcaac ttgaaggtgt cctgtctcca gccatagcga atctcaccta tctccaggtt
      301 cttgatctca cttcaaatag ttttaccggc aaaataccgg ctgaaatagg aaagttaacc
      361 gagcttaacc agcttattct gtacctaaac tatttctctg gttcgattcc ttctggaatc
      421 tgggagctta agaatatttt ctatcttgat cttagaaata atttgttgtc cggtgatgtt
      481 cctgaggaaa tctgcaaaac cagttctttg gtattgattg ggtttgatta caacaactta
      541 accgggaaaa taccagaatg cttaggagat ttggttcatc tccaaatgtt tgtagcagct
      601 ggtaaccatt taactggttc gattccggta tcaattggta ctctggctaa tttaacggat
      661 ttagacctga gtggtaacca gttaaccgga aaaataccga gagattttgg aaatctcttg
      721 aacttacagt ctctcgtttt aactgaaaac ttgttggaag gagatatacc agctgagatc
      781 ggaaactgct cgagcttggt ccaacttgag ctttacgata accagttaac cgggaaaata
      841 ccagctgaat tagggaattt ggttcagctg caagcactcc ggatatacaa gaacaaactt
      901 acttcttcaa ttccatcttc attgttccgg ttaactcagt taacccattt ggggttatca
      961 gaaaaccatt tggttggacc gatatcagaa gaaatcggtt ttcttgagtc acttgaagtc
     1021 ctcacacttc attccaacaa cttcacagga gagtttccac agtccatcac aaacttgagg
     1081 aacttgacag tcctaacggt ggggttcaat aatatttccg gtgagctccc ggcggatcta
     1141 gggcttctta caaaccttcg gaacctttca gcgcacgaca atcttcttac cggaccaata
     1201 ccttccagca taagtaactg caccggtctt aaactcctgg acctgtctca caaccaaatg
     1261 actggcgaga tcccgcgggg tttcggaagg atgaatctta cgttcatttc tattgggagg
     1321 aatcatttca ccggtgaaat tccagatgat atcttcaact gttcaaactt ggaaactctt
     1381 agtgtggcag ataacaactt aacaggaact ctcaagccat taattgggaa gcttcaaaaa
     1441 ctcaggattt tgcaagtttc atataactct ctcactggac cgattcctcg agaaatcggg
     1501 aatctgaaag atttgaatat cttgtacctt cactctaatg gtttcacagg gagaatcccg
     1561 agagagatgt cgaatctcac tctcctccag gggctaagga tgtattcaaa tgatcttgaa
     1621 ggtccaattc ctgaagaaat gtttgatatg aagctactct cagttcttga tctttccaac
     1681 aacaaattct caggtcaaat tcctgccttg ttctccaagc ttgaatcgct tacctacttg
     1741 agtcttcaag gaaacaaatt caacgggtct atccctgcaa gccttaagtc gctttcgctt
     1801 ctcaacacat tcgatatctc cgacaatctt ctcactggaa ccatccctgg agagctgtta
     1861 gcttctttga aaaacatgca gctttacctc aacttctcaa acaacttgtt gactggaacc
     1921 atcccaaagg agcttggaaa gcttgaaatg gttcaagaaa tcgacctttc aaacaatctc
     1981 ttttctgggt ctattccaag atctttacag gcctgcaaaa atgtgttcac actggatttt
     2041 tcgcagaaca atctctcggg tcatatacca gatgaagtct tccaaggcat ggatatgatc
     2101 ataagcttga acctttcaag gaacagtttc tctggagaaa tccctcagag cttcgggaac
     2161 atgacgcatt tggtctcctt ggatctctct agtaacaatc tcactggtga aattccagag
     2221 agtctcgcca atctttcgac tctgaaacat ctcaaactag cttcaaacaa cctcaaaggc
     2281 catgttcctg aatccggggt gttcaaaaac atcaacgcct ctgatctaat gggaaacaca
     2341 gatctctgtg gtagcaagaa gcctctcaag ccatgtacga tcaagcagaa gtcgagccac
     2401 ttctcgaaga gaaccagagt catcctgatt attcttggat cagccgcggc tcttcttctt
     2461 gtcctgcttc ttgttctgat tctaacctgt tgcaagaaaa aagaaaaaaa gattgaaaat
     2521 tcatcagagt cctcattacc ggatttggat tcagctctga aactgaagag atttgaacca
     2581 aaagagttgg agcaagcaac agattcattc aacagtgcca acatcattgg ctcaagcagc
     2641 ttaagcacag tgtacaaagg tcagctagaa gatgggacag tgattgcagt aaaagtattg
     2701 aatctaaagg aattctctgc agaatcagac aagtggttct acacagaagc taaaacattg
     2761 agccaactaa aacatcgaaa cctggtgaag atcttagggt ttgcgtggga aagcggcaaa
     2821 acgaaagctt tagtgcttcc atttatggag aatggaaact tggaggacac cattcacggc
     2881 tctgcagcac cgattgggtc gcttttagaa aaaatcgatc tttgtgttca tatcgcaagc
     2941 ggaatcgatt atcttcattc tggatatggt tttcccatcg ttcattgtga tctgaagcca
     3001 gctaatatac tccttgacag tgaccgcgtt gctcacgtaa gcgattttgg aactgctcgg
     3061 attctaggtt tccgcgaaga tggaagcacc acagcttcaa catcagcctt cgagggtaca
     3121 attggatact tagctccagg taaattactc acaacactta attttcaaaa ccctaattat
     3181 gccatcgact tacttgaaat acactcttat ttttcatgaa cagagtttgc ttatatgagg
     3241 aaagtgacaa caaaagccga tgtattcagc ttcgggatca taatgatgga gctgatgacg
     3301 aaacagagac caacttcgtt gaatgatgaa gattcacaag acatgacttt gcgccaattg
     3361 gtggagaaat cgattggaaa tggaagaaaa gggatggtta gggttcttga tatggaactc
     3421 ggggactcta ttgtttctct gaaacaggaa gaggctattg aagactttct gaagctttgt
     3481 ttgttctgta caagctctag acctgaagat cgacctgata tgaacgagat tcttacacat
     3541 ctgatgaaac ttagaggcaa agcgaattca tttcgagaag atcgtaacga ggatcgagag
     3601 gtgcatatgg gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta
     3661 atctccgagg aagacttgaa cggtgaacaa aaattaatct cagaagaaga cttgaacgga
     3721 gcggtcgaca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     3781 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     3841 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     3901 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     3961 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     4021 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     4081 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     4141 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     4201 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     4261 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     4321 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     4381 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag
     4441 gtcgacctgc aggcatgccc gctgaaatca ccagtctctc tctacaaatc tatctctctc
     4501 tataataatg tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc
     4561 atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca
     4621 ataaaatttc taattcctaa aaccaaaatc caggggtacc ttaattaagc ttggcactgg
     4681 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4741 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4801 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
     4861 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     4921 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     4981 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     5041 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     5101 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     5161 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     5221 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     5281 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     5341 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     5401 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     5461 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     5521 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     5581 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     5641 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     5701 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     5761 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     5821 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     5881 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     5941 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     6001 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     6061 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     6121 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     6181 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     6241 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     6301 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     6361 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     6421 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     6481 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     6541 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     6601 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     6661 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     6721 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     6781 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     6841 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     6901 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     6961 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     7021 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     7081 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     7141 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     7201 ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc
     7261 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
     7321 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     7381 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     7441 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     7501 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     7561 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     7621 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     7681 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     7741 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     7801 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     7861 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     7921 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     7981 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     8041 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     8101 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     8161 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     8221 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     8281 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     8341 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     8401 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     8461 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     8521 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     8581 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     8641 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     8701 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     8761 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
     8821 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     8881 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     8941 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     9001 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     9061 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     9121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9721 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa
     9961 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
    10021 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
    10081 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
    10141 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
    10201 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
    10261 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
    10321 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
    10381 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
    10441 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
    10501 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
    10561 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
    10621 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
    10681 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
    10741 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
    10801 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
    10861 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
    10921 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
    10981 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
    11041 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
    11101 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
    11161 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
    11221 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc
    11281 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
    11341 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
    11401 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagctt
    11461 gtcgatcgac tctagctaga ggatcgatcc gaaccccaga gtcccgctca gaagaactcg
    11521 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
    11581 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
    11641 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
    11701 ccattttcca ccatgatatt cggcaagcag gcatcgccat gtgtcacgac gagatcctcg
    11761 ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
    11821 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
    11881 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
    11941 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
    12001 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
    12061 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc
    12121 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
    12181 gctgacagcc gaaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
    12241 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
    12301 cccatggtcg atcgacagat ctgcgaaagc tcgagagaga tagatttgta gagagagact
    12361 ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
    12421 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca
    12481 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
    12541 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
    12601 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
    12661 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
    12721 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
    12781 gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
    12841 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
    12901 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
    12961 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
    13021 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
    13081 ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac
    13141 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    13201 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    13261 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    13321 aacaatttca cacaggaaac agctatgaca tgattacgaa ttcgaagttg tgaattgtga
    13381 tcataggagt aatatttcta tctgaacaaa aattgaattt tgttttgctt tttgttgaaa
    13441 gataaataaa atgtttggca gattgtttgt acagaacaca acttgttgat aaaacaaatt
    13501 ccatcaagac agctaatgag cttttgatag tcagagcttt gaagacgtgt taatgaatta
    13561 aactgattag acctaaaacc aaatatttag aaagaaaaaa atatgataat tgttatgtta
    13621 tttaatcaat agtcaaatac tatgttgagt accaatcatt tcagtttcaa cgattgctac
    13681 tcttttgacc aataacaaaa aggtttttat ttttcatttc tcaaactcca tttttgtttg
    13741 cttttagcat aaaaacagct agaatgtgag tttcatatgt attatatact ataagaaatc
    13801 atataaatta acgactagaa catattgttt tcattctagt ggtatatctg agtttgacta
    13861 gaacgtttgg aggcagatta aagtgatttc gccttgtcaa aactactttg tatatgttat
    13921 caatcaatcg ctcaaaacta aatcggaaaa aaactacata aataagatat ggaaaatgat
    13981 actaacacaa actagtaagt aagtagtcac tacatattct ggtcccatga atatggctta
    14041 catagtacat acattgtgag attaaatgaa tatgatatat aatacaagca tataagcatg
    14101 tgcatgacta tgaagtggga atacatttag tgcctttata tgcaacttga cagaattacc
    14161 aattatgtga atagtgaaac ttatattgcg tcttggcctt ttcacatccg tgacatgatc
    14221 cctcaaatct atatcttctt gcggcatatg cttatatata aacatatgac ttcgtctata
    14281 aatttatatc cagataatta attctctcat aatatcttct ccacaatttc aactcttcta
    14341 aagtctaaac cggatccgtc taaacc
//
