LOCUS       sequence-169618-       11419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA156/157  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11419)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM156/157
FEATURES             Location/Qualifiers
     source          1..11419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(1334..1993)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1908..1927
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2030..2035,2036..2052,2053..2058))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2331..3038
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3063..3080)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3063..3079)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3072..3094)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3282..3306
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4607..5236
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5665..6738
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6804..6998
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7234..7256)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7342..7482
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7397..7416
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7497..7514)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7668..8256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8343..9137)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9562..9586
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9672..9924)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(9934..10485)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10491..10871)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     10994..11013
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10994..11012
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggcttccatt gcccagctgt
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacgtctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttagtg
      361 ctcactccta tcttctgtca gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatgt gctctctatc tacttctgtc aagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaaa cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gatggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgcgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gcttgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag ttcttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgatctc tagaccatca cattggagct tcagctactc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgtcgac
     1321 aggcctgtac caattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1381 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1441 tcagcacctt gtcgccttgc gtatagtatt tgcccatggt gaaaacgggg gcgaagaagt
     1501 tgtccatatt ggctacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1561 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1621 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1681 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1741 atatcaccag ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc
     1801 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1861 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1921 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1981 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca ttaaaactta
     2041 tcgatgataa gctgtcaaac atgagaattt agcccgccta atgagcgggc tttttgttgt
     2101 gatccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2161 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgttacgta
     2221 gcgatcgcga tcgctacgta acgcctggta tctttatagt cctgtcgccc gggaatttaa
     2281 cccgcctaat gagcgggctt aattaagacc cgggactagt ccctagagtc ctgctttaat
     2341 gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg
     2401 taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga
     2461 atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt
     2521 gtaccctact acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt
     2581 ttatagcgac atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa
     2641 atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct
     2701 tattcaaatt tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta
     2761 ataacgctca ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga
     2821 agttgagtat tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc
     2881 acggcggccg ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg
     2941 tgggccccaa atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc
     3001 cagggcgaat tttgcgacaa catgtcgagg ctcagcagga cctgcaggca tgcaagcttg
     3061 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3121 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3181 cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat
     3241 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
     3301 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     3361 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     3421 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     3481 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     3541 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     3601 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     3661 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     3721 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     3781 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     3841 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     3901 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     3961 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     4021 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     4081 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     4141 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     4201 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     4261 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     4321 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     4381 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     4441 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     4501 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     4561 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     4621 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     4681 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     4741 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     4801 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     4861 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     4921 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     4981 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     5041 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     5101 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     5161 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     5221 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     5281 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     5341 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     5401 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     5461 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     5521 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     5581 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
     5641 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     5701 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     5761 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     5821 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     5881 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     5941 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     6001 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     6061 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     6121 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     6181 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     6241 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     6301 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     6361 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     6421 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     6481 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     6541 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     6601 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     6661 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     6721 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     6781 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     6841 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     6901 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     6961 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     7021 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     7081 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     7141 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     7201 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     7261 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     7321 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     7381 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7441 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     7501 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7561 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7621 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7681 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7741 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7801 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7861 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7921 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7981 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8041 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8101 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8161 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8221 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8281 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
     8341 tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc
     8401 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac
     8461 cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata
     8521 aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa
     8581 aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct
     8641 ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt
     8701 aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg
     8761 tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt
     8821 tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg
     8881 ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc
     8941 catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc
     9001 gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac
     9061 attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat
     9121 attctcattt tagccattta ttatttcctt cctcttttct acagtattta aagatacccc
     9181 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta
     9241 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
     9301 cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc
     9361 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
     9421 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
     9481 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
     9541 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
     9601 acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg
     9661 agctcggatc tgataattta tttgaaaatt cataagaaaa gcaaacgtta catgaattga
     9721 tgaaacaata caaagacaga taaagccacg cacatttagg atattggccg agattactga
     9781 atattgagta agatcacgga atttctgaca ggagcatgtc ttcaattcag cccaaatggc
     9841 agttgaaata ctcaaaccgc cccatatgca ggagcggatc attcattgtt tgtttggttg
     9901 cctttgccaa catgggagtc caagattctg cagtcaaatc tcggtgacgg gcaggaccgg
     9961 acggggcggt accggcaggc tgaagtccag ctgccagaaa cccacgtcat gccagttccc
    10021 gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg cctcgtgcat
    10081 gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga agccctgtgc
    10141 ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc gctggtggcg
    10201 gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg
    10261 gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc agggatagcg
    10321 ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa
    10381 gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc
    10441 ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatcgatt cgatttggtg
    10501 tatcgagatt ggttatgaaa ttcagatgct agtgtaatgt attggtaatt tgggaagata
    10561 taataggaag caaggctatt tatccatttc tgaaaaggcg aaatggcgtc accgcgagcg
    10621 tcacgcgcat tccgttcttg ctgtaaagcg ttgtttggta cacttttgac tagcgaggct
    10681 tggcgtgtca gcgtatctat tcaaaagtcg ttaatggctg cggatcaaga aaaagttgga
    10741 atagaaacag aatacccgcg aaattcaggc ccggttgcca tgtcctacac gccgaaataa
    10801 acgaccaaat tagtagaaaa ataaaaactg actcggatac ttacgtcacg tcttgcgcac
    10861 tgatttgaaa aatctcagaa ttcatggatc ctaggtgagt ctagagagtt aattaagccc
    10921 gctcattagg cgggttaaat tcccgggtac tgcagccgat tcattaatgc aggttaacct
    10981 ggcttatcga aattaatacg actcactata gggagaccgg cagatctgat ccctactcca
    11041 aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
    11101 taatttcggg aaacctcctc ggatcccgtt gcccagctat ctgtcacttc atcgaaagga
    11161 cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca
    11221 ttcaagatgc ctctgccgac ggtggtccca aagatggacc cccacccacg aggagcatcg
    11281 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca
    11341 ctgacgtaag ggatgacgca caatcccacc cctactccaa aaatgtcaaa gatacagtct
    11401 cagaagacca aagggctat
//
