LOCUS       sequence-169683-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 in fission yeast using fluoride selection and targetting
            pil1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Fernandez R, Berro J
  TITLE     Use of a fluoride channel as a new selection marker for fission 
            yeast plasmids and application to fast genome editing with 
            CRISPR/Cas9.
  JOURNAL   Yeast. 2016 Oct;33(10):549-557. doi: 10.1002/yea.3178. Epub 2016 Sep
            7.
  PUBMED    27327046
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3178"; 
            journalName: "Yeast"; date: "2016-10"; volume: "33"; issue: "10"; 
            pages: "549-557"
COMMENT     Sequence Label: pJB172
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      26..807
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
ORIGIN
        1 gacagaatgg gatacaaggg catcgtctat aattatagct aaaaattgta ttttaatttg
       61 tattttttgt aattttattt ctcattgttt tacttaaaat gaatgcgaat tagagaaaac
      121 ttcaacgaaa tgtcaaaata agctcagcaa aatatacaat tttagggaaa gcgatcagca
      181 acttattctc cgttgtagat tctttttctt cataaattcc aacaatgtaa ctgccaattg
      241 atttttctct agatgctaat aaattgaaac taatgaatat aataacacag gttttgataa
      301 attgtaaact cttactcaaa aaaagaatgt taaaactaat ttaacctttt tataaaggct
      361 catggcgtta aagaggatag taaaaattct acaatttttt aatttaatgt cacaattgtc
      421 ttgcgaatat aattctatta attttcgcta actgctgttt tagtactatt actttcaata
      481 tgccttatat gcaactttaa ttcatgaaga atgcaatatt tttgcaatct ttcttgtact
      541 gagcgctttt tatcatttag tactcattat ttaatttttc agtaaagggc aataaataaa
      601 tttgtagaag atgcaatgta atctcctcta tccttttgct catatgttta tgagtatacc
      661 tagtctagaa aggcttgtat ttaaaatatt attcaataaa aattcacaat ttttacgaca
      721 tgtgctacca ttcacttaac ttcctgatta taaaattggt tcgtttatac taattactta
      781 agtaccttta actaaacaaa atgcctatat atatattaat ttacaatgag tgtcagataa
      841 gtcactatgt ccgagtggtt aaggag
//
