LOCUS       sequence-169684-         532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 in fission yeast using fluoride selection and targetting
            pil1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Fernandez R, Berro J
  TITLE     Use of a fluoride channel as a new selection marker for fission 
            yeast plasmids and application to fast genome editing with 
            CRISPR/Cas9.
  JOURNAL   Yeast. 2016 Oct;33(10):549-557. doi: 10.1002/yea.3178. Epub 2016 Sep
            7.
  PUBMED    27327046
REFERENCE   2  (bases 1 to 532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3178"; 
            journalName: "Yeast"; date: "2016-10"; volume: "33"; issue: "10"; 
            pages: "549-557"
COMMENT     Sequence Label: pJB172
FEATURES             Location/Qualifiers
     source          1..532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agcttagcta caaatcccac tggctatatg tatgcatttg tgttaaaaaa gtttgtatag
       61 attatttaat ctactcagca ttctttctct aaataggaat ttgttactta atggagaaaa
      121 aaatgtttcg atttacctag tgtatttgtt tgtatactca cgtttaattt caaacatcca
      181 ttctatcttg tgtaattttt ggcatggtga aaaagataat cagccttata atctttacaa
      241 aagtaagaaa ttctgtaaat aagccttaat gcccttgctt taaattaaaa tggttctttt
      301 tcatgataat gtttgcactt tgtgaatata ttttagatag ttctgtgagg tataattaag
      361 atgttttaga gacttataca attttgtctt tataaattct taattgattt taccatccca
      421 gtttaactat gcttcgtcgg catctctgca catgtcgtgt tttcttaccg tattgtccta
      481 ccaagaacct cttttttgct tggatcgaaa ttaaaggttt aaaagcaaag tt
//
