LOCUS       sequence-169694-        8511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin 
            selection in cells including neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Parker SS, Moutal A, Cai S, Chandrasekaran S, Roman MR, Koshy AA, 
            Khanna R, Zinsmaier KE, Mouneimne G
  TITLE     High Fidelity Cryopreservation and Recovery of Primary Rodent 
            Cortical Neurons.
  JOURNAL   eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-0135-18. doi: 
            10.1523/ENEURO.0135-18.2018. eCollection 2018 Sep-Oct.
  PUBMED    30263951
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eNeuro."; 
            date: "2018-09-27"; volume: "5(5). pii: eN-MNT-0135-18. doi"; pages:
            " 10.1523/ENEURO.0135-18.2018. eCollection 2018 Sep-Oct"
COMMENT     Sequence Label: pLenti Lifeact-mRuby2 BlastR
FEATURES             Location/Qualifiers
     source          1..8511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    226..247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(262..267,268..285,286..292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    300..316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     305..327
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     324..340
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     324..340
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     359..379
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        361..379
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        407..633
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             634..814
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    861..986
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1479..1712
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1897..1941
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2208..2324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2381..2405
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2521..2724
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2675..2695
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2801..2810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2807..2857
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      2873..2882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2879..3589
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     protein_bind    3619..3639
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(3751..3775)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3806..4394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3859..3879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4277..4288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4408..4907
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4443..4466)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4840..4859
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4941..5339
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5436..5669
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5741..5862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5778..5797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5832..5851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5902..6037
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6057..6076)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6058..6076)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6086..6103)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6086..6102)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6095..6117)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6244..6699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6331..6350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6541..6562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6725..6829
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6830..6898,6899..7690)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7048..7067)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7861..8449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8350..8369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      241 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      301 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      361 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
      421 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      481 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      541 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      601 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      661 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      721 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      781 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      841 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      901 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      961 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     1021 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1081 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1141 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1201 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1261 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1321 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1381 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1441 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1501 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1561 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1621 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1681 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1741 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1801 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1861 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1921 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1981 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2041 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2101 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2161 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     2221 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2281 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc cagtgtggtg
     2341 gaattgagta ttccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc
     2401 aggctggaga cccaagcttg gtaccgagct cggatccact agtattatgc ccagtacatg
     2461 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2521 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2581 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2641 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2701 tgggaggttt atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagattc tagagctagc gccaccatgg gcgtggccga
     2821 cttgatcaag aagttcgagt ccatctccaa ggaggagggg gatccaccgg tcgccaccat
     2881 ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg
     2941 ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg
     3001 aactcaaacc atgaggatca aagtcatcga gggaggaccc ctgccatttg cctttgacat
     3061 tcttgccacg tcgttcatgt atggcagccg tacttttatc aagtacccga aaggcattcc
     3121 tgatttcttt aaacagtcct ttcctgaggg ttttacttgg gaaagagtta cgagatacga
     3181 agatggtgga gtcgtcaccg tcatgcagga caccagcctt gaggatggct gtctcgttta
     3241 ccacgtccaa gtcagagggg taaactttcc ctccaatggt cccgtgatgc agaagaagac
     3301 caagggttgg gagcctaata cagagatgat gtatccagca gatggtggtc tgaggggata
     3361 cactcatatg gcactgaaag ttgatggtgg tggccatctg tcttgctctt tcgtaacaac
     3421 ttacaggtca aaaaagaccg tcgggaacat caagatgccc ggtatccatg ccgttgatca
     3481 ccgcctggaa aggttagagg aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc
     3541 agttgccaag ttcgccgggc ttggtggtgg gatggacgag ctgtacaagt aagcggcctc
     3601 gagcatgcat ctagaggaca actttgtata caaaagttgg gagacccaag cttggtaccg
     3661 agctcggatc cactagtaac ggccgccagt gtgctggaat tctgcagata tccatcacac
     3721 tggcggccgc tcgagcatgc atctagaggt acccagcttt cttgtacaaa gtggttgata
     3781 tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt
     3841 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3901 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3961 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4021 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4081 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4141 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4201 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4261 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4321 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4381 ggccgcctcc ccgcctggaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     4441 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     4501 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     4561 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     4621 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     4681 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     4741 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     4801 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     4861 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     4921 gcttaccaca ctcctgcagg atggccaagc ctttgtctca agaagaatcc accctcattg
     4981 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     5041 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     5101 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     5161 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     5221 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     5281 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     5341 cacgcgtatg catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt
     5401 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg
     5461 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5521 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5581 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5641 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     5701 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     5761 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5821 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     5881 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5941 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6001 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     6061 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6121 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6181 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6241 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6421 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6541 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6601 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6661 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     6721 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6781 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6841 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6901 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6961 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7021 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7081 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7141 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7201 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7261 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7321 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7381 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7441 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7501 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7561 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7621 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7681 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7741 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7801 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7861 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7921 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7981 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8041 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8101 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8161 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8221 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8281 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8341 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8401 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8461 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
