LOCUS       sequence-169695-        8723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin 
            selection in cells including neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Padilla-Rodriguez M, Parker SS, Adams DG, Westerling T, Puleo JI, 
            Watson AW, Hill SM, Noon M, Gaudin R, Aaron J, Tong D, Roe DJ, 
            Knudsen B, Mouneimne G
  TITLE     The actin cytoskeletal architecture of estrogen receptor positive 
            breast cancer cells suppresses invasion.
  JOURNAL   Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2.
  PUBMED    30061623
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-30"; volume: "9(1):2980. doi"; pages: " 
            10.1038/s41467-018-05367-2"
COMMENT     Sequence Label: pLenti Lifeact-iRFP670 BlastR
FEATURES             Location/Qualifiers
     source          1..8723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..537
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(73..96)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     470..489
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             571..969
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1066..1299
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1371..1492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1408..1427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1462..1481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1532..1667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1687..1706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1688..1706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1716..1733)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1716..1732)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1725..1747)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1874..2329
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1961..1980)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2171..2192
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2355..2459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2460..2528,2529..3320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2678..2697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3491..4079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3980..3999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4233..4250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4367..4388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4403..4408,4409..4426,4427..4433)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4441..4457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4446..4468
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4465..4481
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4465..4481
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4500..4520
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4502..4520
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4548..4774
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4775..4955
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5002..5127
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5620..5853
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6038..6082
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6349..6465
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6522..6546
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6662..6865
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6816..6836
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6862..6886
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6942..6951
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6948..6998
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      7014..7023
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7020..7955
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     protein_bind    7972..7992
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(8104..8128)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(8159..8723,1..24)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8212..8232)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8630..8641)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tctccctttg ggccgcctcc ccgcctggaa ttctaccggg taggggaggc gcttttccca
       61 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
      121 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
      181 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
      241 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
      301 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
      361 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
      421 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
      481 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
      541 tgcagcccaa gcttaccaca ctcctgcagg atggccaagc ctttgtctca agaagaatcc
      601 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
      661 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
      721 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
      781 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
      841 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
      901 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
      961 gagggctaag cacgcgtatg catctcgagg gcccggtacc tttaagacca atgacttaca
     1021 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc
     1081 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     1141 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1201 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1261 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     1321 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     1381 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     1441 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     1501 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     1561 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     1621 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     1681 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     1741 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     1801 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     1861 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1921 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1981 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2041 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     2101 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2161 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     2221 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     2281 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     2341 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2401 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2461 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2521 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2581 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2641 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2701 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2761 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     2821 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2881 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     2941 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3001 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3061 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3121 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3181 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3241 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3301 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3361 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3421 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3481 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3541 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3601 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3661 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3721 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3781 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3841 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3901 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3961 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4021 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4081 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4141 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4201 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4261 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4321 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     4381 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     4441 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     4501 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     4561 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     4621 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     4681 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     4741 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     4801 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     4861 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     4921 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     4981 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     5041 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     5101 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     5161 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     5221 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     5281 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     5341 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     5401 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     5461 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     5521 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     5581 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     5641 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     5701 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     5761 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     5821 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     5881 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     5941 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     6001 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     6061 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     6121 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     6181 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     6241 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     6301 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
     6361 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     6421 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcgt ccagtgtggt
     6481 ggaattgagt attccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag
     6541 caggctggag acccaagctt ggtaccgagc tcggatccac tagtattatg cccagtacat
     6601 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     6661 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     6721 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     6781 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     6841 gtgggaggtt tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     6901 tccacgctgt tttgacctcc atagaagatt ctagagctag cgccaccatg ggcgtggccg
     6961 acttgatcaa gaagttcgag tccatctcca aggaggaggg ggatccaccg gtcgccacca
     7021 tggcgcgtaa ggtcgatctc acctcctgcg atcgcgagcc gatccacatc cccggcagca
     7081 ttcagccgtg cggctgcctg ctagcctgcg acgcgcaggc ggtgcggatc acgcgcatta
     7141 cggaaaatgc cggcgcgttc tttggacgcg aaactccgcg ggtcggtgag ctactcgccg
     7201 attacttcgg cgagaccgaa gcccatgcgc tgcgcaacgc actggcgcag tcctccgatc
     7261 caaagcgacc ggcgctgatc ttcggttggc gcgacggcct gaccggccgc accttcgaca
     7321 tctcactgca tcgccatgac ggtacatcga tcatcgagtt cgagcctgcg gcggccgaac
     7381 aggccgacaa tccgctgcgg ctgacgcggc agatcatcgc gcgcaccaaa gaactgaagt
     7441 cgctcgaaga gatggccgca cgggtgccgc gctatctgca ggcgatgctc ggctatcacc
     7501 gcgtgatgtt gtaccgcttc gcggacgacg gctccgggat ggtgatcggc gaggcgaagc
     7561 gcagcgacct cgagagcttt ctcggtcagc actttccggc gtcgctggtc ccgcagcagg
     7621 cgcggctact gtacttgaag aacgcgatcc gcgtggtctc ggattcgcgc ggcatcagca
     7681 gccggatcgt gcccgagcac gacgcctccg gcgccgcgct cgatctgtcg ttcgcgcacc
     7741 tgcgcagcat ctcgccctgc catctcgaat ttctgcggaa catgggcgtc agcgcctcga
     7801 tgtcgctgtc gatcatcatt gacggcacgc tatggggatt gatcatctgt catcattacg
     7861 agccgcgtgc cgtgccgatg gcgcagcgcg tcgcggccga aatgttcgcc gacttcttat
     7921 cgctgcactt caccgccgcc caccaccaac gctaagcatg catctagagg acaactttgt
     7981 atacaaaagt tgggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc
     8041 agtgtgctgg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
     8101 ggtacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgacaa
     8161 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     8221 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     8281 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     8341 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     8401 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     8461 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     8521 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     8581 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8641 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8701 ccttcgccct cagacgagtc gga
//
