LOCUS       sequence-169696-        8518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFP-tagged Lifeact with Blasticidin 
            selection in cells including neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Padilla-Rodriguez M, Parker SS, Adams DG, Westerling T, Puleo JI, 
            Watson AW, Hill SM, Noon M, Gaudin R, Aaron J, Tong D, Roe DJ, 
            Knudsen B, Mouneimne G
  TITLE     The actin cytoskeletal architecture of estrogen receptor positive 
            breast cancer cells suppresses invasion.
  JOURNAL   Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2.
  PUBMED    30061623
REFERENCE   2  (bases 1 to 8518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-30"; volume: "9(1):2980. doi"; pages: " 
            10.1038/s41467-018-05367-2"
COMMENT     Sequence Label: pLenti Lifeact-EGFP BlastR
FEATURES             Location/Qualifiers
     source          1..8518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..79
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             161..559
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             656..889
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    961..1082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(998..1017)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1052..1071
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1122..1257
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1277..1296)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1278..1296)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1306..1323)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1306..1322)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1315..1337)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1464..1919
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1551..1570)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1761..1782
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1945..2049
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2050..2118,2119..2910)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2268..2287)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3081..3669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3570..3589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3823..3840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3957..3978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3993..3998,3999..4016,4017..4023)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4031..4047
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4036..4058
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4055..4071
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4055..4071
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4090..4110
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4092..4110
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4138..4364
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4365..4545
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4592..4717
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5210..5443
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5628..5672
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5939..6055
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6112..6136
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6252..6455
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6406..6426
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6452..6476
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6532..6541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6538..6588
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      6604..6613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6610..6612,6613..6615,6616..7329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6610..6612,6613..6615,6616..7326)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6655..6676)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6916..6935)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7263..7284
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    7357..7377
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(7489..7513)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7544..8132
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7597..7617)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8015..8026)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(8146..8518,1..127)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8181..8204)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
       61 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      121 cctttcgacc tgcagcccaa gcttaccaca ctcctgcagg atggccaagc ctttgtctca
      181 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
      241 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
      301 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
      361 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
      421 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
      481 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
      541 ttatgtgtgg gagggctaag cacgcgtatg catctcgagg gcccggtacc tttaagacca
      601 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
      661 gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
      721 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      781 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      841 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
      901 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
      961 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     1021 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     1081 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     1141 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     1201 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     1261 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     1321 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     1381 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     1441 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     1501 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     1561 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     1621 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     1681 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     1741 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     1801 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     1861 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     1921 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1981 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2041 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     2101 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2161 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     2221 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     2281 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     2341 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2401 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2461 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2521 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2581 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2641 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2701 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2761 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2821 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2881 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2941 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3001 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3841 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3901 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3961 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     4021 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     4081 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     4141 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     4201 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     4261 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     4321 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     4381 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4441 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4501 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     4561 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     4621 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     4681 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     4741 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     4801 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     4861 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     4921 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     4981 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     5041 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     5101 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     5161 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     5221 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     5281 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     5341 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     5401 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     5461 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     5521 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     5581 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     5641 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     5701 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     5761 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     5821 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     5881 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     5941 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     6001 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcgt
     6061 ccagtgtggt ggaattgagt attccagtgt ggtggaattc tgcagatatc aacaagtttg
     6121 tacaaaaaag caggctggag acccaagctt ggtaccgagc tcggatccac tagtattatg
     6181 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     6241 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     6301 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     6361 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     6421 ggcgtgtacg gtgggaggtt tatataagca gagctcgttt agtgaaccgt cagatcgcct
     6481 ggagacgcca tccacgctgt tttgacctcc atagaagatt ctagagctag cgccaccatg
     6541 ggcgtggccg acttgatcaa gaagttcgag tccatctcca aggaggaggg ggatccaccg
     6601 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     6661 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     6721 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     6781 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     6841 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     6901 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     6961 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     7021 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     7081 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     7141 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     7201 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     7261 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     7321 tacaagtaaa gcggcctcga gcatgcatct agaggacaac tttgtataca aaagttggga
     7381 gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc
     7441 tgcagatatc catcacactg gcggccgctc gagcatgcat ctagaggtac ccagctttct
     7501 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gacaatcaac ctctggatta
     7561 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7621 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7681 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7741 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7801 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7861 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7921 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     7981 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     8041 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     8101 gagtcggatc tccctttggg ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc
     8161 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     8221 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     8281 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     8341 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     8401 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     8461 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccggggg
//
