LOCUS       sequence-169709-       11031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of demethylase-inactive KDM3A H1120G/D1122N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Wade MA, Jones D, Wilson L, Stockley J, Coffey K, Robson CN, Gaughan
            L
  TITLE     The histone demethylase enzyme KDM3A is a key estrogen receptor 
            regulator in breast cancer.
  JOURNAL   Nucleic Acids Res. 2015 Jan;43(1):196-207. doi: 10.1093/nar/gku1298.
            Epub 2014 Dec 8.
  PUBMED    25488809
REFERENCE   2  (bases 1 to 11031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1298"; journalName: "Nucleic Acids Res"; date: 
            "2015-01"; volume: "43"; issue: "1"; pages: "196-207"
COMMENT     Sequence Label: pLenti-V5-KDM3A-mut
FEATURES             Location/Qualifiers
     source          1..11031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(221..245)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             298..339
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(376..396)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        381..710
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      561..696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        758..805
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             824..1222
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1309..1542
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1614..1735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1651..1670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1705..1724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1775..1910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1930..1949)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1931..1949)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1959..1976)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1959..1975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1968..1990)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2117..2572
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2204..2223)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2414..2435
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2598..2702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2703..2771,2772..3563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2921..2940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3734..4322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4223..4242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4476..4493
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4610..4631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4646..4651,4652..4669,4670..4676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4684..4700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4689..4711
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4708..4724
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4708..4724
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4743..4763
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4745..4763
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4791..5017
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5018..5198
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5245..5370
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5863..6096
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6281..6325
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        6604..6907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6908..7111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7062..7082
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7108..7132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7228..7252
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttatatagct gcatcaaagt ggctgaagat tttgtttctc cagagcatgt taaacactgc
       61 ttctggctta ctcaggaatt ccgatatctg tcacagactc ataccaatca cgaagataaa
      121 ttacaggtga agaatgttat ctaccatgca gtgaaagatg cagttgctat gctgaaagcc
      181 agtgaatcca gttttggcaa acctaagggt gggcgcgccg acccagcttt cttgtacaaa
      241 gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
      301 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt
      361 tggaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
      421 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
      481 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      541 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      601 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
      661 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
      721 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
      781 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc
      841 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
      901 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
      961 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     1021 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     1081 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     1141 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     1201 tggttatgtg tgggagggct aagcacaatt cgagctcggt acctttaaga ccaatgactt
     1261 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     1321 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     1381 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     1441 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     1501 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     1561 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     1621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1741 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1801 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1861 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     1921 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     1981 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2041 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2101 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2161 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2221 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2281 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     2341 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2401 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     2461 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     2521 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     2581 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2641 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2701 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2761 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2821 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2881 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2941 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3001 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3061 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3121 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3181 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3241 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3301 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3361 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3421 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3481 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3541 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3601 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3661 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3721 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3781 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3841 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3901 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3961 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4081 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4261 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4321 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4381 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4441 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4501 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4561 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     4621 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     4681 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     4741 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     4801 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     4861 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     4921 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     4981 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     5041 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5101 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5161 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     5221 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     5281 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     5341 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     5401 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     5461 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     5521 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     5581 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     5641 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     5701 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     5761 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     5821 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     5881 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     5941 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     6001 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     6061 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     6121 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     6181 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     6241 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     6301 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     6361 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     6421 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     6481 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     6541 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt
     6601 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     6661 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     6721 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     6781 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     6841 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     6901 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     6961 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     7021 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     7081 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     7141 acgccatcca cgctgttttg acctccatag aagacaccga ctctagagga tccactagtc
     7201 cagtgtggtg gaattctgca gatatcaaca agtttgtaca aaaaagcagg ctccgcggcc
     7261 gcccccttca ccatggtgct cacgctcgga gaaagttggc cggtattggt ggggaggagg
     7321 tttctcagtc tgtccgcagc cgacggcagc gatggcagcc acgacagctg ggacgtggag
     7381 cgcgtcgccg agtggccctg gctctccggg accattcgag ctgtttccca caccgacgtt
     7441 accaagaagg atctgaaggt gtgtgtggaa tttgatgggg aatcttggag gaaaagaaga
     7501 tggatagaag tctacagcct tctaaggaga gcatttttag tagaacataa tttggtttta
     7561 gctgaacgaa agtcacctga aatttctgaa cgaattgtac agtggcctgc aataacgtac
     7621 aaacctctgt tggacaaagc tggtttggga tccataactt ctgttcgctt tctgggagat
     7681 caacaaagag tatttctttc taaagacctt ttgaagccta tacaggatgt aaacagtctt
     7741 cgactttctc ttacggataa tcagattgtc agtaaagaat ttcaagcttt gattgtgaag
     7801 catttagatg aaagccatct tttaaaaggt gacaaaaact tagttggttc agaagtaaaa
     7861 atttatagct tggacccatc tactcagtgg ttttcagcaa ccgttataaa tggaaaccca
     7921 gcatcaaaaa ctcttcaagt caactgtgag gagattccag cactgaaaat tgttgatccg
     7981 tcactgattc atgctgaagt tgtacacgat aaccttgtga catgtggtaa ttctgcaaga
     8041 attggagctg taaaacgcaa gtcttctgag aataatggaa ccctggtttc caaacaagca
     8101 aaatcttgct ctgaggcctc tcccagtatg tgtcctgtgc agtctgtacc tacaacagtt
     8161 tttaaggaga tactgcttgg ctgtactgca gcaactccac ctagtaagga cccaagacag
     8221 caaagtactc cccaggctgc caactctcca cctaaccttg gagcaaaaat tcctcaagga
     8281 tgtcataaac aaagtttacc agaggaaatt tcttcctgtc taaatacaaa gtctgaagct
     8341 ctgagaacaa aaccagatgt ctgcaaagca gggttgctct caaagtcctc tcagattgga
     8401 actggagact tgaaaattct gactgagcca aaaggcagct gtactcagcc taagacaaac
     8461 actgatcagg aaaacagatt ggagtctgtt ccacaagcat tgactggcct tcctaaggag
     8521 tgcttaccta caaaggcttc ttctaaggca gaattggaaa ttgccaatcc tcctgaactg
     8581 cagaagcacc tagaacatgc accttcccca tcggatgttt caaatgcacc agaagtgaaa
     8641 gcaggtgtca atagtgatag ccctaataac tgttcaggaa aaaaggtaga accttcagct
     8701 ttagcttgcc gatcacagaa tttaaaggaa tcttcagtaa aagtagataa tgaaagctgt
     8761 tgttcaagaa gcaacaataa aatccagaat gccccatcca ggaagtcggt tttgacagac
     8821 ccagctaaac tcaaaaagct gcaacagagt ggcgaggcct tcgtacagga tgattcttgt
     8881 gtgaacatcg tggcacagtt gcctaaatgc cgagagtgtc gcttggacag tctccgcaag
     8941 gataaggagc aacagaagga ctcacctgtg ttttgccgct tctttcactt caggaggtta
     9001 caattcaaca aacatggtgt gttgcgggta gaaggcttct taacaccaaa caagtatgac
     9061 aatgaagcaa ttggcttgtg gttaccttta accaaaaacg ttgtggggat tgatttggac
     9121 acagcaaagt acatcttggc caacattgga gaccacttct gtcaaatggt gatttctgaa
     9181 aaggaagcta tgtcaactat tgagccacac agacaggttg cttggaagcg agctgtcaaa
     9241 ggtgttcgag aaatgtgtga tgtgtgcgac accaccatct tcaacctgca ctgggtgtgt
     9301 cctcggtgtg ggtttggagt atgtgtggac tgctaccgga tgaagagaaa gaattgccaa
     9361 cagggtgctg cttacaagac tttctcttgg ctaaaatgtg tgaagagtca gatacatgaa
     9421 ccagagaact taatgcccac acagatcatt cctggaaaag cactctatga tgttggagac
     9481 attgttcatt ctgtaagagc gaaatgggga ataaaggcaa actgcccctg ctcaaacagg
     9541 caattcaaac tcttttcaaa gccagcctca aaggaagacc taaaacagac ttctttagct
     9601 ggagaaaaac cgactcttgg tgcagtgctc cagcagaatc cctcagtgtt ggagccagca
     9661 gctgtgggtg gggaagcagc ctccaagcca gccggcagca tgaagcctgc ctgtccagcc
     9721 agcacatctc ctctaaactg gctggccgac ctaaccagcg ggaatgtcaa caaggaaaac
     9781 aaggaaaaac aaccaacaat gccaatttta aagaatgaaa tcaaatgcct tccacccctc
     9841 ccacctttaa gcaaatccag cacagtcctc catacgttta acagcacaat tttgacaccc
     9901 gtaagcaaca acaattctgg tttcctccgg aatctcttga attcttctac aggaaagaca
     9961 gaaaatggac tcaagaatac accaaaaatc cttgatgaca tctttgcctc tttggtgcaa
    10021 aataagacga cttctgattt atctaagagg cctcaaggac taaccatcaa gcccagcatt
    10081 ctgggctttg acactcctca ctattggctt tgtgataatc gcttgctgtg cttgcaagac
    10141 cccaacaata agagcaactg gaatgtgttt agggagtgct ggaaacaagg gcagccagtg
    10201 atggtgtctg gagtgcatca taaattgaac tctgaacttt ggaaacctga atccttcagg
    10261 aaagagtttg gtgagcagga agtagaccta gttaattgta ggaccaatga aatcatcaca
    10321 ggagccacag taggagactt ctgggatgga tttgaagatg ttccaaatcg tttgaaaaat
    10381 gaaaaagaac caatggtgtt gaaacttaag gactggccac caggagaaga ttttagagat
    10441 atgatgcctt ccaggtttga tgatctgatg gccaacattc cactgcccga gtacacaagg
    10501 cgagatggca aactgaattt ggcctctagg ctgccaaact actttgttcg gccagatctg
    10561 ggccccaaga tgtataatgc ttatggatta atcactcctg aagatcggaa atatggaaca
    10621 acaaatcttg gtttaaatgt atctgatgca gctaatgtca tggtctatgt gggaattccc
    10681 aaaggacagt gtgagcaaga agaagaagtc cttaagacca tccaagatgg agattctgac
    10741 gaactcacaa taaagcgatt tattgaagga aaagagaagc caggagcact gtggcacata
    10801 tatgctgcaa aggacacgga gaagataagg gaatttctta aaaaggtatc agaagagcaa
    10861 ggtcaagaaa acccagtaga ccacgatcct attcatgatc aaagctggta tttagaccga
    10921 tcattaagaa aacgtcttca tcaagagtat ggagttcaag gctgggctat tgtacagttt
    10981 cttggggatg tggtgtttat cccggcagga gctccacatc aggttcataa c
//
